本文選題：細(xì)胞周期蛋白A + 骨髓增生異常綜合征�。� 參考：《中國實驗血液學(xué)雜志》2017年06期

【摘要】：目的:通過小干擾RNA(siRNA)下調(diào)Cyclin A1的表達(dá),觀察其對骨髓增生異常綜合征(MDS)細(xì)胞系SKM-1細(xì)胞增殖的影響并探討其作用機制。方法:采用陽離子脂質(zhì)體轉(zhuǎn)染方法,在SKM-1細(xì)胞中轉(zhuǎn)染Cyclin A1的siRNA;轉(zhuǎn)染48 h后收集細(xì)胞,應(yīng)用Western blot及RT-PCR測定轉(zhuǎn)染效率;應(yīng)用CCK-8方法檢測細(xì)胞增殖,Western blot及RT-PCR檢測Cyclin A1基因沉默前后CDK2、RUNX1、SRSF2基因表達(dá)的水平。結(jié)果:轉(zhuǎn)染Cyclin A1特異性siRNA 48 h后,Cyclin A1蛋白及mRNA表達(dá)水平顯著下降(P0.01)。下調(diào)Cyclin A1后,SKM-1細(xì)胞的增殖受抑,CDK2、RUNX1及SRSF2基因的蛋白及mRNA表達(dá)量均下調(diào)(P0.05)。結(jié)論:Cyclin A1表達(dá)下調(diào)后,SKM-1細(xì)胞的增殖受到抑制,表明Cyclin A1可能是MDS治療的潛在靶點之一。
[Abstract]:Aim: to investigate the effect of small interfering RNA-siRNAs (siRNAs) on the proliferation of Cyclin A1 cell line SKM-1 cells and its mechanism. Methods: the siRNAs of Cyclin A1 were transfected into SKM-1 cells by cationic liposome transfection, and the transfection efficiency was measured by Western blot and RT-PCR after 48 h transfection. CCK-8 and RT-PCR were used to detect the expression of CDK2 / RUNX1 / SRSF2 gene before and after Cyclin A1 gene silencing. Results: the expression of Cyclin A1 protein and mRNA decreased significantly 48 h after transfection of Cyclin A1 specific siRNA. After down-regulation of Cyclin A1, the proliferation of SKM-1 cells was inhibited and the protein and mRNA expression of RUNX1 and SRSF2 genes were down-regulated. Conclusion the proliferation of SKM-1 cells is inhibited after the down-regulation of the expression of Cyclin A1, which suggests that Cyclin A1 may be one of the potential targets of MDS therapy.
【作者單位】： 北京大學(xué)人民醫(yī)院北京大學(xué)血液病研究所;北京大學(xué)國際醫(yī)院血液科;
【基金】：北京市自然科學(xué)基金(7122190) 教育部留學(xué)回國人員科研啟動基金(教司留-賈晉松)
【分類號】：R551.3

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