本文選題：酒石酸布托啡諾 + 心肌再灌注損傷��； 參考：《東南大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年01期

【摘要】：目的:探討酒石酸布托啡諾預(yù)處理對(duì)小鼠心肌缺血再灌注損傷的保護(hù)作用及其機(jī)制。方法:取雄性C57BL/6小鼠30只,隨機(jī)分為3組,每組10只。缺血再灌注組(I/R組)結(jié)扎左冠狀動(dòng)脈前降支(LAD)30 min,再灌注6 h;布托啡諾預(yù)處理組(B+I/R組)在缺血前30 min經(jīng)后肢肌肉注射酒石酸布托啡諾40μg·kg-1,余處理同I/R組;假手術(shù)組(Sham組)開胸暴露心臟,僅LAD穿線但不結(jié)扎。Sham組和I/R組小鼠在缺血前30 min肌肉注射等體積生理鹽水。再灌注6 h后,采集腹主動(dòng)脈血樣,酶聯(lián)免疫吸附法測(cè)定血清腫瘤壞死因子α(TNF-α)、肌酸激酶同工酶(CK-MB)和肌鈣蛋白Ⅰ(CTnⅠ)的濃度;Western blot法測(cè)定心肌組織Cleaved caspase 8、Cleaved caspase 3、p-JNK和JNK的表達(dá);凝膠電泳遷移率(EMSA)法測(cè)定心肌組織核因子-κB(NF-κB)結(jié)合活性。結(jié)果:與Sham組比較,I/R組血清CK-MB、CTn I和TNF-α水平顯著升高(均P0.05),心肌組織Cleaved caspase 8和Cleaved caspase 3蛋白表達(dá)水平、JNK磷酸化水平以及NF-κB結(jié)合活性顯著升高(均P0.05);與I/R組比較,B+I/R組血清CK-MB、CTn I和TNF-α水平顯著下降(均P0.05),心肌組織Cleaved caspase 8和Cleaved caspase 3蛋白表達(dá)、JNK磷酸化水平和NF-κB結(jié)合活性顯著下降(均P0.05)。結(jié)論:酒石酸布托啡諾預(yù)處理對(duì)小鼠心肌缺血再灌注損傷具有保護(hù)作用,可顯著抑制I/R所致的炎癥反應(yīng)和心肌細(xì)胞凋亡,其機(jī)制與阻滯JNK/NF-κB信號(hào)通路相關(guān)。
[Abstract]:Aim: to investigate the protective effect and mechanism of butorphanol tartrate pretreatment on myocardial ischemia reperfusion injury in mice. Methods: thirty male C57BL/6 mice were randomly divided into 3 groups with 10 mice in each group. The left anterior descending branch of the coronary artery was ligated for 30 minutes and reperfusion for 6 hours, and butorphanol was injected intramuscularly through the hind limb 30 min before ischemia into the butorphanol 40 渭 g kg-1. The remaining treatment was the same as that in the I / R group. The heart was exposed to open chest in sham-operated group. Only mice in the LAD puncture group but not ligated. Sham group and I / R group were intramuscularly injected with normal saline 30 min before ischemia. After reperfusion for 6 h, the blood samples of abdominal aorta were collected. The concentrations of serum TNF- 偽, creatine kinase isoenzyme (CK-MBB) and troponin 鈪，

本文編號(hào)：1871777