發(fā)布時間：2018-05-10 21:56

  本文選題：大鼠 + 竇房結(jié)細(xì)胞　； 參考：《西安交通大學(xué)學(xué)報(醫(yī)學(xué)版)》2017年06期

【摘要】：目的建立急性分離成年大鼠竇房結(jié)細(xì)胞的方法,了解其形態(tài)結(jié)構(gòu)特點,系統(tǒng)觀察鈉通道各亞型在竇房結(jié)細(xì)胞的表達(dá)情況,為進(jìn)一步研究鈉通道在竇房結(jié)功能中的作用及分子機(jī)制提供依據(jù)。方法取成年大鼠竇房結(jié)組織,Ⅱ型膠原蛋白酶聯(lián)合蛋白酶酶解分離活性竇房結(jié)細(xì)胞,行鈉通道亞型Nav1.1、Nav1.2、Nav1.3、Nav1.5、Nav1.6、Nav1.7、Nav1.8、Nav1.9的免疫熒光染色,激光共聚焦顯微鏡觀察各亞型在竇房結(jié)細(xì)胞的表達(dá)。結(jié)果在急性分離的竇房結(jié)細(xì)胞中觀察到梭形、弧形、細(xì)長彎曲形等細(xì)胞形態(tài),以梭形為主,細(xì)胞橫紋清楚、活性好,可存活6~8h;鈉通道Nav1.1、Nav1.5、Nav1.6、Nav1.7、Nav1.8、Nav1.9在大鼠竇房結(jié)細(xì)胞呈陽性表達(dá),Nav1.2、Nav1.3表達(dá)呈陰性。結(jié)論Ⅱ型膠原蛋白酶聯(lián)合蛋白酶酶解分離方法是可靠的成年大鼠活性竇房結(jié)細(xì)胞分離方法,鈉通道各亞型在成年大鼠竇房結(jié)細(xì)胞呈差異性表達(dá)。
[Abstract]:Objective to establish a method for the acute isolation of sinoatrial node cells from adult rats, to understand their morphological and structural characteristics, and to observe systematically the expression of sodium channel subtypes in sinus node cells. To further study the role of sodium channel in sinoatrial node function and the molecular mechanism. Methods the active sinoatrial node cells were isolated from the sinoatrial node tissue of adult rats by enzymatic hydrolysis of type 鈪，

本文編號：1871053