本文選題：Klotho蛋白 + 血管平滑肌細(xì)胞　； 參考：《南京醫(yī)科大學(xué)》2015年碩士論文

【摘要】：目的:探討Klotho蛋白對(duì)高磷誘導(dǎo)的大鼠血管平滑肌細(xì)胞成骨樣轉(zhuǎn)化、鈣化作用的影響及可能的機(jī)制。方法:體外培養(yǎng)大鼠的血管平滑肌細(xì)胞,用10mmol/L的β甘油磷酸誘導(dǎo)其鈣化,在培養(yǎng)基中加入不同濃度的重組小鼠Klotho蛋白(rm Klotho),并加或不加Wnt/β-catenin通路激動(dòng)劑Li Cl。干預(yù)12天后,茜素紅染色觀察鈣鹽沉積,分光光度計(jì)測(cè)定細(xì)胞外基質(zhì)鈣含量。Western印記法分別檢測(cè)骨形態(tài)形成蛋白2(BMP2)、Runt相關(guān)轉(zhuǎn)錄因子2(Runx2)、β連環(huán)蛋白(β-catenin)的表達(dá),免疫熒光法檢測(cè)α-SMA的表達(dá)。結(jié)果:β甘油磷酸誘導(dǎo)大鼠血管平滑肌細(xì)胞12天后,茜素紅染色鈣鹽沉積明顯,細(xì)胞外基質(zhì)鈣含量增加,細(xì)胞BMP2,Runx2,β-catenin表達(dá)較正常對(duì)照組上調(diào),α-SMA表達(dá)較正常對(duì)照下調(diào)。而在加rm Klotho蛋白干預(yù)后,細(xì)胞鈣化減輕,BMP2,Runx2,β-catenin表達(dá)均下調(diào),α-SMA表達(dá)上調(diào)。但是,Klotho干預(yù)的同時(shí)加Li Cl,則BMP2、Runx2及β-catenin表達(dá)較Klotho干預(yù)組上調(diào)。結(jié)論:Klotho蛋白能減輕高磷誘導(dǎo)的血管平滑肌細(xì)胞的成骨細(xì)胞轉(zhuǎn)化、鈣化作用,且這一保護(hù)作用可能與Wnt/β-catenin通路的抑制有關(guān)。目的:探討容量超負(fù)荷(FO)水平在評(píng)價(jià)危重病患者預(yù)后中的意義。方法:對(duì)2013年3月至2013年10月在南京醫(yī)科大學(xué)第一附屬醫(yī)院綜合ICU住院的100例危重患者,評(píng)估其病程中的FO水平,分析FO與APACHEⅡ評(píng)分及危重癥患者的住院病死率、ICU治療時(shí)間、機(jī)械通氣時(shí)間等主要預(yù)后指標(biāo)的關(guān)系。結(jié)果:100例患者存活80例,死亡20例,病死率為20.0%。其中死亡組APACHEⅡ評(píng)分值和FO水平均高于存活組(P0.01)。APACHEⅡ評(píng)分、ICU治療時(shí)間、機(jī)械通氣時(shí)間及病死率隨著FO水平升高而升高。結(jié)論:FO和APACHEⅡ評(píng)分的升高對(duì)危重癥患者的存活、機(jī)械通氣時(shí)間、ICU治療時(shí)間等主要預(yù)后指標(biāo)的預(yù)測(cè)有重要意義。
[Abstract]:Aim: to investigate the effect of Klotho protein on osteoblast-like transformation and calcification of rat vascular smooth muscle cells induced by high phosphorus and its possible mechanism. Methods: rat vascular smooth muscle cells were cultured in vitro. Calcification was induced by 尾 -glycerophosphoric acid (尾 -glycerophosphoric acid) of 10mmol/L. Different concentrations of recombinant mouse Klotho protein rm Klothotrol were added to the medium and Wnt/ 尾 -catenin pathway agonist LiCl was added or not. After 12 days of intervention, calcium deposition was observed by alizarin red staining. The expression of bone morphogenetic protein 2BMP2U Runt, 尾 -catenin and 偽 -SMA were detected by spectrophotometer and immunofluorescence respectively. Results: after 12 days of 尾 -glycerophosphoric acid induced vascular smooth muscle cells, alizarin red staining calcium salt deposition was obvious, extracellular matrix calcium content increased, BMP2 + Runx2, 尾 -catenin expression was higher than normal control group, 偽 -SMA expression was down-regulated. However, after the addition of RM Klotho protein, calcification decreased the expression of BMP2nRunx2 and 尾 -catenin, and up-regulated the expression of 偽 -SMA. However, the expression of BMP2 + Runx2 and 尾 -catenin in Klotho treated group was higher than that in Klotho group. Conclusion Klotho protein can attenuate the osteoblast transformation and calcification of vascular smooth muscle cells induced by high phosphorus, and this protective effect may be related to the inhibition of Wnt/ 尾 -catenin pathway. Objective: to evaluate the prognostic value of volume overload FOO level in critically ill patients. Methods: from March 2013 to October 2013, 100 critically ill patients with ICU in the first affiliated Hospital of Nanjing Medical University were enrolled. The FO levels in the course of the disease were evaluated, and the scores of FO and APACHE 鈪，

本文編號(hào)：1834069