本文選題：高效液相色譜技術(shù) + 異常血紅蛋白病　； 參考：《中國實驗血液學(xué)雜志》2017年06期

【摘要】：目的:應(yīng)用高效液相色譜技術(shù)(HPLC)檢測廣州市育齡期夫婦異常血紅蛋白病的發(fā)生情況。方法:對2008年10月至2016年2月參加廣州市出生缺陷干預(yù)工程和免費孕前健康體檢的育齡期夫婦篩查,應(yīng)用HPLC檢測各種異常血紅蛋白,應(yīng)用Gap-PCR檢測法和反向斑點雜交法(RDB)檢測常見α、β地中海貧血基因型。結(jié)果:HPLC檢測樣本為11712份,發(fā)現(xiàn)134例共8種異常血紅蛋白,分別為Hb E 102例(17例合并α地中海貧血),Hb Q-Thailand 20例(18例合并-α~(4.2)/αα,1例合并-α~(4.2)/αα和CD17/N,1例合并-α~(4.2)/αα和βE/N),Hb D-Iran 4例(2例合并-α~(3.7)/αα,1例合并CD41-42/N),Hb G-Honolulu 3例(1例合并-α~(3.7)/αα),Hb J-Bangkok 2例(均合并--~(SEA)/αα),Hb Koln 1例(合并--~(SEA)/αα),Hb Osu-Christiansborg 1例(合并--~(SEA)/αα),Hb Hasharon 1例(合并-α~(3.7)/αα)。異常血紅蛋白病的檢出率為1.14%(134/11712)。結(jié)論:廣州地區(qū)血紅蛋白病的發(fā)生率較高,共檢出8種異常血紅蛋白。應(yīng)用HPLC技術(shù)對育齡期夫婦進行異常血紅蛋白病的篩查,可做到早發(fā)現(xiàn),早預(yù)防,對優(yōu)生優(yōu)育和提高人口素質(zhì)具有重要意義。
[Abstract]:Objective: to detect abnormal hemoglobin in couples of childbearing age in Guangzhou by high performance liquid chromatography (HPLC). Methods: from October 2008 to February 2016, couples of childbearing age who participated in the intervention project of birth defects and free pre-pregnancy health examination in Guangzhou were screened, and the abnormal hemoglobin was detected by HPLC. Common 偽, 尾 thalassemia genotypes were detected by Gap-PCR and reverse dot blot hybridization. Results 11712 samples were detected by WHP-HPLC, and 8 kinds of abnormal hemoglobin were found in 134 cases. 鍒嗗埆涓篐b E 102渚，

本文編號：1793172