本文選題：超聲波 + 輻照　； 參考：《遵義醫(yī)學院》2017年碩士論文

【摘要】：目的:觀察不同劑量超聲波輻照對蛙腿骨骼肌力學狀態(tài)的影響;量化評價超聲波輻照誘導蛙腿骨骼肌力學變化的特征;應(yīng)用組織多普勒超聲成像觀察超聲波輻照對蛙腿骨骼肌電機械興奮性及收縮性的影響。方法:(1)制備60個蛙離體坐骨神經(jīng)-腓腸肌標本,分為A、A’、B、B’四組(15只/組),其中A、B為實驗組,A’、B’分別為A、B的空白對照組。實驗組接受不同超聲波輻照劑量(A組輻照劑量為3.0W/cm2、90s,B組輻照劑量為1.0W/cm2、60s)。將Powerlab多道生理信號采集處理系統(tǒng)與標本連接,發(fā)放電刺激坐骨神經(jīng)誘導腓腸肌收縮,在基礎(chǔ)狀態(tài)張力數(shù)據(jù)采集后進行超聲波輻照,在輻照后第0、第5、第10及第20分鐘重復進行張力數(shù)據(jù)采集。評價以上各組張力測值的時間變化規(guī)律以及組間差異。(2)另行制備36個蛙離體坐骨神經(jīng)-腓腸肌標本,分組(A組和B組各10只/組,A’組和B’組各8只/組)、干預措施及觀察時間點同上。所有標本連接Powerlab多道生理信號采集處理系統(tǒng),同時通過導聯(lián)線與彩超儀心電通道連接以輸入同步電刺激信號。Powerlab發(fā)放電脈沖刺激坐骨神經(jīng)誘導腓腸肌收縮。應(yīng)用M型彩色組織多普勒及頻譜多普勒組織顯像同步觀察標本腓腸肌在干預前后各個觀察時間點的電機械延遲時間(t1)、機械收縮持續(xù)時間(t2)及收縮速度(v)。分析評價以上各組三個參數(shù)的時間變化規(guī)律及組間差異。結(jié)果:(1)A組和A’組張力測值差異無統(tǒng)計學意義(P0.05);但交互效應(yīng)顯著(P0.05),對照組A’張力隨著時間延長而減低,實驗組A張力隨著時間延長呈增加趨勢。組內(nèi)比較,A組第5分鐘張力較基礎(chǔ)狀態(tài)增高,差異有統(tǒng)計學意義(P0.05)。B組和B’組各觀察時間點張力測值差異無統(tǒng)計學意義(P0.05);B組與B’組張力均隨時間延長而減低。(2)○1 t1:A組和A’組時間效應(yīng)、交互效應(yīng)及分組效應(yīng)差異均無統(tǒng)計學意義(P0.05);B組和B’組時間效應(yīng)差異有統(tǒng)計學意義(P0.05),交互效應(yīng)及分組效應(yīng)差異無統(tǒng)計學意義(P0.05)�！�2 t2:A組和A’組間交互效應(yīng)及時間效應(yīng)差異有統(tǒng)計學意義,A’組較A組隨時間延長t2呈明顯增加趨勢(P0.05),分組效應(yīng)差異無統(tǒng)計學意義(P0.05);B組和B’組時間效應(yīng)、交互效應(yīng)及分組效應(yīng)差異均無統(tǒng)計學意義(P0.05)�！�3 v:A組和A’組間時間效應(yīng)差異有統(tǒng)計學意義,交互效應(yīng)及分組效應(yīng)差異無統(tǒng)計學意義;B組和B’組時間效應(yīng)、交互效應(yīng)及分組效應(yīng)均差異無統(tǒng)計學意義。所有組別t1、t2測值均隨時間延長呈增加趨勢,v測值隨時間延長呈降低趨勢。結(jié)論:當超聲波輻照強度為3.0W/cm2、輻照時間90s時,對蛙腿骨骼肌張力有一定的正性肌力作用,輻照后第5分鐘肌力明顯增高。本研究采用的超聲波輻照條件對電機械延遲時間、機械收縮持續(xù)時間和收縮速度無顯著影響,空白對照組(A’組)較實驗組(A組,輻照強度為3.0W/cm2、輻照時間90s)t2增加趨勢更明顯,間接表明超聲波輻照對標本可產(chǎn)生一定正性作用。
[Abstract]:Objective: to observe the effects of different doses of ultrasonic irradiation on the mechanical state of frog leg skeletal muscle, and to evaluate quantitatively the characteristics of the mechanical changes of frog leg skeletal muscle induced by ultrasonic irradiation. The effects of ultrasonic irradiation on the electromechanical excitability and contraction of frog leg skeletal muscle were observed by tissue Doppler ultrasound imaging. Methods 60 isolated Frog sciatic nerve and gastrocnemius muscle specimens were prepared. The experimental group received different doses of ultrasound irradiation. The radiation dose of group A was 3.0 W / cm ~ (2) ~ (90) s ~ (-1). The dose of group B was 1.0 W / cm ~ (2 +) ~ (60) s ~ (-1). The Powerlab multi-channel physiological signal acquisition and processing system was connected with the specimen, and the sciatic nerve was stimulated to induce the contraction of gastrocnemius muscle. Tension data acquisition was repeated at 0, 5, 10 and 20 minutes after irradiation. To evaluate the time variation of tension values and the differences between groups, 36 isolated frog sciatic nerve and gastrocnemius muscle specimens were prepared. Group A and group B were divided into two groups: group A (n = 10) and group B (n = 8). All the specimens were connected to the Powerlab multi-channel physiological signal acquisition and processing system. At the same time, the synchronous electrical stimulation signal .Powerlab was used to stimulate the sciatic nerve to induce gastrocnemius muscle contraction by using the lead wire and the ECG channel of the color ultrasound instrument. M-color tissue Doppler and spectral Doppler tissue imaging were used to observe the electromechanical delay time (t _ 1), mechanical contraction duration (t _ 2) and contraction velocity of gastrocnemius muscle before and after intervention. To analyze and evaluate the time variation of the three parameters and the differences between the three groups. Results there was no significant difference in tension between group A and group A (P 0.05), but the interaction effect was significant (P 0.05). The tension of group A decreased with time, and tension of group A increased with time. The tension in group A at 5 minutes was higher than that in basic state. There was no significant difference in the observed point tension values between group B and group B (P 0.05). The tension of group B and group B 'decreased with time, and the time effect of group A and group A' decreased with the prolongation of time. There was no significant difference in the interaction effect and the group effect. There was no significant difference in the time effect between the B group and the B 'group. There was no significant difference in the interaction effect and the group effect. There was no significant difference in the interaction effect between group A and group A and the interaction effect between group A and group A' was timely. There was significant difference in the effect between group A and group A (P 0.05), but there was no significant difference between group A and group B (P 0.05), and there was no significant difference in group B and group B'in time effect between group A 'and group A (P < 0.05), but there was no significant difference between group A and group A (P < 0.05). There was no significant difference in the time effect between group A and group A, but there was no significant difference in time effect between group B and group A, but there was no significant difference in time effect between group A and group A, but there was no significant difference in time effect between group B and group B', but there was no significant difference in time effect between group A and group A. There was no significant difference in interaction effect and group effect. The values of T1 and T2 in all groups showed an increasing trend with time prolonging and the values of v showed a decreasing trend with time prolonging. Conclusion: when the intensity of ultrasound irradiation is 3.0 W / cm ~ 2 and irradiation time is 90 s, it has a positive effect on the skeletal muscle tension of frog leg, and the muscle strength of frog leg increases obviously at 5 minutes after irradiation. The ultrasonic irradiation conditions adopted in this study had no significant effect on the electromechanical delay time, mechanical contraction duration and contraction velocity. The intensity of irradiation was 3.0W / cm ~ 2, and the increasing trend of irradiation time 90s)t2 was more obvious than that of group A (blank control group). The results showed that ultrasonic irradiation had positive effect on the specimen.
【學位授予單位】：遵義醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R541.6;R445.1

【相似文獻】

相關(guān)期刊論文 前10條

1 胡俊;張琳琳;夏晶;陳新山;;固定不同時間對兔骨骼肌肌動蛋白免疫組化染色影響的研究[J];中國組織化學與細胞化學雜志;2007年06期

2 袁建琴;劉承宜;徐曉陽;;骨骼肌肌酸轉(zhuǎn)運及其分子機制[J];中國運動醫(yī)學雜志;2009年03期

3 井上勛;;骨骼肌興奮——收縮耦聯(lián)的進化[J];瀘州醫(yī)學院學報;1993年04期

4 張曉峰;石松;魏永旺;;骨骼肌的運動適應(yīng)機制[J];中國組織工程研究與臨床康復;2011年46期

5 郭宏波;轉(zhuǎn)基因的骨骼肌細胞及其在I型糖尿病治療中的應(yīng)用[J];臨床和實驗醫(yī)學雜志;2002年04期

6 都愛蓮,任惠民,呂傳真,盧家紅,趙重波,朱雯華;25kD蛋白在人骨骼肌中的定位及在人其他組織中的表達[J];神經(jīng)解剖學雜志;2004年02期

7 蒙碧輝,劉紅,梁瑩,黃松,冼蘇;1型糖尿病樹，

本文編號：1780603