本文選題：貝前列腺素鈉　切入點：低氧性肺動脈高壓　出處：《西安交通大學(xué)學(xué)報(醫(yī)學(xué)版)》2017年04期

【摘要】：目的探討貝前列素鈉(BPS)對低氧性肺動脈高壓(HPH)大鼠的療效,及其對肺動脈平滑肌氧敏感性KV通道表達(dá)的影響。方法采用在低壓低氧艙內(nèi)每日飼養(yǎng)8h的方法建立HPH大鼠模型;BPS干預(yù)組每日給予BPS灌胃[300μg/(kg·d)],對照組和模型組給予生理鹽水灌胃;4周后測定平均肺動脈壓力(mPAP),計算右心肥厚指數(shù)(RVHI),肺組織切片HE染色評價肺動脈壁增厚程度,Real-time PCR和Western blot檢測肺小動脈平滑肌KV1.2、KV1.5、KV2.1mRNA和蛋白表達(dá)水平。結(jié)果連續(xù)4周低壓低氧成功誘導(dǎo)HPH大鼠模型;與模型組相比,BPS干預(yù)后mPAP和RVHI降低[mPAP:(13.48±2.18)mmHg vs.(23.87±2.23)mmHg vs.(17.09±1.20)mmHg;RVHI:0.28±0.02 vs.0.46±0.03 vs.0.36±0.04;P0.05];肺小動脈重構(gòu)改善(中膜面積百分比:35.72±6.58 vs.68.52±5.64 vs.46.58±8.43,P0.05),肺動脈平滑肌KV1.2、KV1.5、KV2.1mRNA和蛋白表達(dá)水平升高(蛋白相對表達(dá)量:KV1.2,0.78±0.10 vs.0.15±0.03 vs.0.57±0.13;KV1.5,0.61±0.10 vs.0.31±0.05 vs.0.59±0.13;KV2.1,0.29±0.05 vs.0.10±0.02 vs.0.28±0.07;P0.05)。結(jié)論 BPS可改善HPH大鼠的肺動脈高壓,并上調(diào)肺動脈平滑肌KV1.2、KV1.5、KV2.1的表達(dá)。
[Abstract]:Objective to investigate the effect of berprostatin sodium (BPS) on hypoxic pulmonary hypertension (HPH) rats. Methods HPH rat model was established by feeding in hypobaric hypoxia chamber for 8 hours daily. HPH rats were treated with BPS daily [300 渭 g/(kg d], and the control group and model group were treated with BPS. The mean pulmonary artery pressure (MPP) was measured after 4 weeks of gastric perfusion with saline. The right cardiac hypertrophy index (RVHI) was calculated. The pulmonary artery wall thickening degree was evaluated by HE staining. The mRNA and protein expression of pulmonary arteriolar smooth muscle was detected by real-time PCR and Western blot. HPH rat model was successfully induced by hypobaric hypoxia for 4 weeks. Compared with model group, mPAP and RVHI decreased [mPAP:(13.48 鹵2.18)mmHg vs.(23.87 鹵2.23)mmHg vs.(17.09 鹵1.20mm vs.(17.09 鹵1.20mm vs.(17.09 鹵0.13KV 鹵0.03 vs.0.36 鹵0.04P 0.05], pulmonary arterioles remodeling improved (percentage of middle membrane area 35.72 鹵6.58 vs.68.52 鹵8.43P0.05), and expression of KV1.2mV1.5kV1.5KV2.1mRNA and protein increased (relative expression of KV1.278 鹵0.10 vs.0.15 鹵0.03#en10# 鹵0.13KV 1.5o.61 鹵0.10#en11# 鹵0.05 vs.0.59 鹵0.13kV2.1a 鹵0.29 鹵0.05 vs.0.10 鹵0.02 vs.0.28 鹵0.07P0.05P0.05a). Conclusion BPS can improve pulmonary hypertension in HPH rats. The expression of KV 1. 2 and KV 1. 5 in pulmonary artery smooth muscle was upregulated.
【作者單位】： 西安交通大學(xué)第一附屬醫(yī)院心血管內(nèi)科;陜西省人民醫(yī)院心血管內(nèi)科;
【基金】：國家自然科學(xué)基金資助項目(No.81170294)~~
【分類號】：R544.1

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