發(fā)布時間：2018-03-23 04:29

  本文選題：FTY　切入點：糖尿病　出處：《中國動脈硬化雜志》2017年05期 　論文類型：期刊論文

【摘要】：目的研究1-磷酸鞘氨醇受體激動劑芬戈莫德(FTY720)對糖尿病大鼠早期心肌微血管病變的作用。方法將Spragure-Dawley大鼠分為正常對照組、糖尿病模型組與FTY720[0.001 g/(kg·d)]治療組。大劑量鏈脲佐菌素(65 mg/kg)腹腔注射制備大鼠糖尿病模型,HE、Masson染色觀察心肌結(jié)構(gòu)大體形態(tài)。CD34免疫組織化學(xué)觀察心肌微血管密度,TUNEL免疫熒光檢測分析心臟微血管內(nèi)皮細胞凋亡情況。組織轉(zhuǎn)化生長因子β(TGF-β)、免疫組織化學(xué)觀察心肌纖維化程度。Western blot測定血管細胞黏附分子1(VCAM-1)、腫瘤壞死因子α(TNF-α)和白細胞介素6(IL-6)表達水平。結(jié)果糖尿病模型組以多次隨機血糖≥16.7 mmol/L作為糖尿病模型成功的標志,本研究中大鼠糖尿病模型構(gòu)建成功率在85%以上。與正常對照組相比,糖尿病模型組大鼠心肌組織微血管壁增厚,VCAM-1、TNF-α和IL-6的表達升高(P0.05);FTY720治療組心肌VCAM-1、TNF-α與IL-6的表達明顯低于糖尿病模型組(P0.05)。糖尿病模型組心臟組織微血管密度減少,凋亡指數(shù)顯著增高;而FTY720可以減少凋亡,促進內(nèi)皮細胞增殖。結(jié)論 FTY720可能在糖尿病大鼠心臟微血管損傷中具有一定保護作用。
[Abstract]:Objective to study the effect of 1-sphingosine phosphate receptor agonist, Fengomod FTY720, on early myocardial microangiopathy in diabetic rats. Methods Spragure-Dawley rats were divided into normal control group. Diabetic model group and FTY720 [0.001 g/(kg d] treatment group. High dose streptozotocin 65 mg / kg intraperitoneal injection was used to make diabetic model in rats. Observation of myocardial structure and morphology. CD34 immunohistochemical method was used to observe myocardial microvessel density and Tunel immunofluorescence. Detection and analysis of apoptosis in cardiac microvascular endothelial cells. Tissue transforming growth factor 尾 -TGF- 尾, immunohistochemical observation of myocardial fibrosis degree. Western blot detection of vascular cell adhesion molecule 1 VCAM-1, tumor necrosis factor 偽 TNF- 偽) and interleukin 6 IL-6) tables. Results multiple random blood glucose 鈮，

本文編號：1651918