本文選題：大鼠　切入點：炎癥　出處：《廣西醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:探討高脂聯(lián)合維生素D3飲食對大鼠動脈組織AU堿基富集區(qū)結(jié)合因子1(AUF1)及白細(xì)胞介素35(IL-35)表達(dá)的影響。方法:SD大鼠20只隨機分入實驗組和正常組,每組10只。實驗組進行高脂飼料聯(lián)合維生素D3喂養(yǎng)12周,取其動脈組織,以正常飼養(yǎng)大鼠動脈組織為對照,常規(guī)HE染色觀察各組動脈組織病變情況,用實時定量PCR法分別檢測動脈組織AUF1及IL-35的mRNA表達(dá);蛋白質(zhì)免疫印跡法分別檢測動脈組織AUF1及IL-35蛋白表達(dá)。結(jié)果:實驗組動脈組織出現(xiàn)輕微病變,血脂異常,AUF1存在mRNA表達(dá)上調(diào)(P0.01);炎癥抑制因子IL-35 mRNA存在表達(dá)上調(diào)。(P0.01)。各組AUF1及IL-35蛋白未見高濃度表達(dá)。結(jié)論:高脂高鈣飲食能誘導(dǎo)大鼠動脈炎癥,但不足以短期內(nèi)使大鼠形成典型動脈粥樣硬化斑塊。AUF1和IL-35可作為未來治療炎癥的治療靶點。
[Abstract]:Objective: to investigate the effects of hyperlipidemia combined with vitamin D3 diet on the expression of AU-AUF1) and IL-35 in rat arterial tissue. Methods: twenty SD rats were randomly divided into experimental group and normal group. 10 rats in each group were fed with high fat diet combined with vitamin D 3 for 12 weeks. The arterial tissue was taken from the experimental group, and the arterial tissue of the normal feeding rats was used as the control. The pathological changes of the arteries in each group were observed by routine HE staining. The mRNA expression of AUF1 and IL-35 in arterial tissues was detected by real-time quantitative PCR, and the expression of AUF1 and IL-35 in arterial tissues was detected by Western blot. The expression of mRNA was up-regulated in AUF1, and the expression of IL-35 mRNA was up-regulated. There was no high expression of AUF1 and IL-35 in all groups. Conclusion: high fat and high calcium diet can induce arterial inflammation in rats. But it is not enough to form typical atherosclerotic plaques. AUF1 and IL-35 in the short term can be used as therapeutic targets for the treatment of inflammation in the future.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R54

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