本文選題：型糖尿病　切入點：冠狀動脈粥樣硬化性心臟病　出處：《山東醫(yī)藥》2017年12期 　論文類型：期刊論文

【摘要】：目的篩選2型糖尿病(T2DM)合并冠狀動脈粥樣硬化性心臟病(簡稱冠心病)患者與單純T2DM患者血漿差異表達的微小RNA(miRNA),分析可能用于判斷T2DM合并冠心病的潛在生物標記物。方法選取T2DM患者60例,根據(jù)是否合并冠心病分為T2DM合并冠心病組和單純T2DM組,每組30例。收集兩組臨床資料,采用微陣列miRNA芯片篩查兩組差異表達的miRNA,采用實時熒光定量RT-PCR法檢測兩組差異表達miRNA的相對表達量(驗證微陣列芯片結果),分析差異表達miRNA與T2DM患者臨床參數(shù)的關系。結果微陣列miRNA芯片數(shù)據(jù)篩選出兩組存在7個差異表達的候選miRNA,即miR-765、miR-320c、miR-33b-3p、miR-21-5P、miR-22-3p、miR-20b-5p及miR-92a-3p。與單純T2DM組比較,T2DM合并冠心病組miR-765、miR-320c相對表達量降低(P均0.01)。T2DM患者血漿miR-765、miR-320c相對表達量與Hb A1C均呈負相關(β分別為-0.379、-0.192,P均0.01),與LDL-C均呈負相關(β分別為-0.224、-0.232,P均0.05)。結論 miR-765和miR-320c在T2DM合并冠心病患者與單純T2DM患者血漿中存在差異表達,二者表達量降低提示T2DM患者有合并冠心病的可能。
[Abstract]:Objective to screen small RNAs miRNAs differentially expressed in plasma between patients with coronary atherosclerotic heart disease (CHD) and simple T2DM patients with type 2 diabetes mellitus (T2DM), and to analyze the potential biomarkers of T2DM with coronary heart disease (CHD). Methods 60 patients with T2DM were selected, According to whether coronary heart disease is complicated or not, it is divided into two groups: T2DM with coronary heart disease group and simple T2DM group with 30 cases in each group. The clinical data of the two groups are collected. Two groups of differentially expressed miRNAs were screened by microarray miRNA chip, and the relative expression of differentially expressed miRNA was detected by real-time fluorescence quantitative RT-PCR method. The clinical parameters of differentially expressed miRNA and T2DM patients were analyzed. Results the miRNAs with seven differentially expressed miRNAs were screened by microarray miRNA chip data, namely, miR-765miR-320canmiR-33b-33b-3pnmiR-21-5PnmiR-22-3pnmiR-20b-5p and miR-92a-3p.Compared with T2DM group, the relative expression of miR-765miR-320c was decreased in T2DM patients with coronary heart disease (P < 0.01). The relative expression of miR-765miR-320c and miR-765miR-320c in T2DM patients with coronary heart disease were significantly lower than those in T2DM group (P < 0.05). HbA1C was negatively correlated (尾 = -0.379- 0.192P, P = 0.01g, P = -0.224- 0.232P, P = 0.050.Conclusion there is a difference in the expression of miR-765 and miR-320c in plasma between T2DM patients with coronary heart disease and those with T2DM alone. The decrease of the expression of both suggests that type 2 DM patients may be associated with coronary heart disease.
【作者單位】： 首都醫(yī)科大學公共衛(wèi)生學院;首都醫(yī)科大學附屬北京潞河醫(yī)院;
【基金】：國家自然科學基金資助項目(31672375/30971178)
【分類號】：R587.1;R541.4

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