本文選題：可溶性環(huán)氧化物水解酶抑制劑　切入點(diǎn)：內(nèi)皮-間質(zhì)轉(zhuǎn)化　出處：《鄭州大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年02期 　論文類型：期刊論文

【摘要】：目的:觀察可溶性環(huán)氧化物水解酶抑制劑t-AUCB對(duì)TGF-β1誘導(dǎo)的人臍靜脈內(nèi)皮細(xì)胞(HUVEC)內(nèi)皮-間質(zhì)轉(zhuǎn)化(End MT)的影響。方法:采用t-AUCB(50μmol/L)預(yù)處理HUVEC 40 min后,用TGF-β1(10μg/L)誘導(dǎo)HUVEC 24 h,采用Western blot法檢測(cè)Smad2/3的磷酸化水平;誘導(dǎo)72 h后,光鏡觀察細(xì)胞形態(tài)的變化,采用CCK-8試劑盒檢測(cè)細(xì)胞活性,采用實(shí)時(shí)熒光定量PCR檢測(cè)內(nèi)皮細(xì)胞標(biāo)記物(CD31)、間質(zhì)細(xì)胞標(biāo)記物(collagenⅠ、collagenⅢ、vimentin)及End MT中重要的下游轉(zhuǎn)錄因子(snail1、twist1、twist2、ZEB1)mRNA的表達(dá)。同時(shí)設(shè)TGF-β1和tAUCB單獨(dú)作用組。結(jié)果:光鏡下,TGF-β1組細(xì)胞轉(zhuǎn)變?yōu)楠M長形,細(xì)胞間隙疏松,TGF-β1+t-AUCB組、t-AUCB組細(xì)胞形態(tài)正常。TGF-β1組細(xì)胞CD31 mRNA表達(dá)下調(diào),collagenⅠ、collagenⅢ、vimentin mRNA表達(dá)上調(diào),snail1、twist1、twist2、ZEB1 mRNA表達(dá)上調(diào),Smad2及Smad3蛋白磷酸化水平升高(P0.05)。與TGF-β1組比較,TGF-β1+t-AUCB組、t-AUCB組上述指標(biāo)的表達(dá)均有一定程度的逆轉(zhuǎn)(P0.05)。結(jié)論:t-AUCB可通過抑制內(nèi)皮細(xì)胞的End MT而發(fā)揮抗纖維化作用。
[Abstract]:Aim: to observe the effect of t-AUCB, a soluble epoxide hydrolase inhibitor, on endothelium-mesenchymal transformation of human umbilical vein endothelial cells (HUVECs) induced by TGF- 尾 1. Methods: HUVEC was pretreated with t-AUCB50 渭 mol / L for 40 min. HUVEC was induced by TGF- 尾 1 10 渭 g / L for 24 h, and the phosphorylation level of Smad2/3 was detected by Western blot assay. After 72 h of induction, the changes of cell morphology were observed under light microscope, and the cell activity was detected by CCK-8 kit. Real time fluorescence quantitative PCR was used to detect the mRNA expression of endothelial cell marker CD31, interstitial cell marker collagen 鈪，

本文編號(hào)：1603172