發(fā)布時(shí)間：2018-03-12 15:34

  本文選題：SIRT1　切入點(diǎn)：高血壓性腦出血　出處：《福建中醫(yī)藥大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:探討SIRT1 rs2273773、rs7069102、rs7895833基因多態(tài)性與高血壓性腦出血的相關(guān)性,為該病的預(yù)防和康復(fù)預(yù)后提供臨床基礎(chǔ)。方法:本研究采用病例-對(duì)照研究方法,收集201例高血壓性腦出血患者及203例種族、年齡、性別匹配的對(duì)照組為研究對(duì)象,用聚合酶鏈反應(yīng)-限制性片段長度多態(tài)性方法分析SIRT1基因rs2273773、rs7069102、rs7895833多態(tài)性,我們測(cè)定了入院時(shí)血壓值以及外周血FBG、TG、TC、HDLC、LDLC等,對(duì)高血壓性腦出血患者進(jìn)行NIHSS量表評(píng)定,使用SPSS20.0軟件進(jìn)行統(tǒng)計(jì)分析,比較SIRT1基因rs2273773、rs7069102、rs7895833多態(tài)性在病例組和對(duì)照組之間的分布情況,同時(shí)比較不同基因型間血壓、空腹血糖及生化指標(biāo)水平的差異,在高血壓性腦出血組中比較不同基因型間NIHSS評(píng)分水平的差異。結(jié)果:1、高血壓性腦出血組(Hypertensive Cerebral Hemorrhage,HICH)與對(duì)照組間年齡、性別無顯著性差異(P0.05),但吸煙史、飲酒史、糖尿病史、FBG在高血壓性腦出血組明顯高于對(duì)照組(P0.01);TC、HDLC在高血壓性腦出血組明顯低于對(duì)照組(P0.05)。2、SIRT1基因rs2273773、rs7069102多態(tài)性基因型頻率及等位基因頻率在高血壓性腦出血組與對(duì)照組間無顯著差異(P0.05),而在高血壓性腦出血患者組中SIRT1基因rs7895833多態(tài)性AA基因型及A等位基因頻率均顯著高于對(duì)照組(P0.05)。非條件logistic回歸分析顯示,以GG基因型為對(duì)照,高血壓性腦出血組的AA基因型發(fā)病風(fēng)險(xiǎn)升高有關(guān)(P=0.008,OR=3.360,95%CI:1.370-8.239),另外以G等位基因?yàn)閷?duì)照,高血壓性腦出血組攜帶A等位基因的發(fā)病風(fēng)險(xiǎn)升高(P=0.048,OR=1.369,95%CI:1.003-1.869)。3、SIRT1 基因 rs2273773、rs7069102 及 rs7895833 位點(diǎn)各基因型與血壓、FBG、生化指標(biāo)進(jìn)行分析,發(fā)現(xiàn)rs7895833位點(diǎn)在SBP水平呈AAAGGG趨勢(shì),rs2273773位點(diǎn)在HDLC水平呈CCCTTT趨勢(shì),差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。在高血壓性腦出血組中rs2273773、rs7069102及rs7895833位點(diǎn)各基因型與NIHSS得分進(jìn)行對(duì)比無統(tǒng)計(jì)學(xué)差異(P0.05)。4、多因素logistic回歸分析高血壓性腦出血的相關(guān)危險(xiǎn)因素表明,甘油三脂、低密度脂蛋白、SBP、rs7895833多態(tài)性是高血壓性腦出血的獨(dú)立危險(xiǎn)因素(p0.05),攜帶rs7895833AA型和A等位基因的患者罹患高血壓性腦出血的發(fā)病率高,而高密度脂蛋白為高血壓腦出血組的獨(dú)立保護(hù)因素(P0.05)。結(jié)論:1、SIRT1基因可能是福建漢族人群高血壓性腦出血的的遺傳易感基因,其rs7895833位點(diǎn)AA基因型和等位基因A攜帶者增加高血壓性腦出血的發(fā)病風(fēng)險(xiǎn),但尚需擴(kuò)大樣本量進(jìn)一步證實(shí)。2、SIRT1基因rs7895833多態(tài)性與收縮壓水平相關(guān),rs2273773多態(tài)性與HDLC水平有關(guān),而SIRT1基因rs2273773、rs7069102及rs7895833多態(tài)性與高血壓性腦出血組功能障礙程度(NIHSS評(píng)分)可能無關(guān)。
[Abstract]:Objective: To investigate the SIRT1 rs2273773, rs7069102, rs7895833 gene polymorphism in patients with hypertensive cerebral hemorrhage, and provide clinical basis for the prevention and rehabilitation and prognosis of the disease. Methods: This study used a case-control study, collected 201 cases of patients with hypertensive cerebral hemorrhage and 203 cases of race, age, sex matched as control group the object of study, using polymerase chain reaction restriction fragment length polymorphism analysis of SIRT1 gene rs2273773, rs7069102, rs7895833 polymorphism, we measured blood pressure and peripheral blood FBG, TG on admission, TC, HDLC, LDLC, NIHSS were assessed in patients with hypertensive cerebral hemorrhage, was used for statistical analysis the software of SPSS20.0, SIRT1 genes rs2273773, rs7069102, the distribution of rs7895833 polymorphism between the patient group and control group, and the comparison between different genotypes and biochemical indexes of blood pressure, fasting blood sugar water Flat differences, differences in the level of NIHSS score in group comparison among different genotypes of hypertensive cerebral hemorrhage. Results: 1 hypertensive cerebral hemorrhage group (Hypertensive Cerebral, Hemorrhage, HICH) and the control group had no significant difference between age, gender (P0.05), but the smoking history, drinking history, history of diabetes, FBG in hypertensive cerebral hemorrhage group was significantly higher than the control group (P0.01); TC, HDLC in hypertensive cerebral hemorrhage group was significantly lower than the control group (P0.05.2), SIRT1 gene rs2273773, rs7069102 polymorphism genotypes and allele frequencies in hypertensive cerebral hemorrhage had no significant difference between the group and the control group (P0.05). In SIRT1 group and rs7895833 gene polymorphism of AA genotype and A allele frequency of hypertensive cerebral hemorrhage were significantly higher than control group (P0.05). Non conditional logistic regression analysis showed that GG genotype on hypertensive cerebral hemorrhage group, AA gene The increased risk of type (P=0.008, OR=3.360,95%CI:1.370-8.239), in addition to the G allele were increased the risk of hypertensive cerebral hemorrhage group carrying the A allele (P=0.048, OR=1.369,95%CI:1.003-1.869).3, SIRT1 rs2273773 and rs7069102 gene, rs7895833 locus genotypes and FBG analysis, blood pressure, biochemical index, rs7895833 sites were AAAGGG trend in the level of SBP, rs2273773 loci showed CCCTTT trend in the HDLC level, the differences were statistically significant (P0.05). In hypertensive cerebral hemorrhage group in rs2273773, rs7069102 and rs7895833 loci genotypes and NIHSS score were compared no significant difference (P0.05.4), logistic regression analysis of related risk of cerebral hemorrhage the factors of hypertension showed that glycerin three fat, low density lipoprotein, SBP, rs7895833 polymorphism is an independent risk factor of hypertension cerebral hemorrhage (P0.05), Carrying the rs7895833AA genotype and A allele in patients with hypertension cerebral hemorrhage rate is high, and high density lipoprotein is an independent protective factor for hypertension cerebral hemorrhage group (P0.05). Conclusion: 1. The SIRT1 gene may be a susceptible gene in genetic hypertensive cerebral hemorrhage in Fujian Han population, the rs7895833 locus AA genotype and allele A carriers increased risk of hypertensive cerebral hemorrhage, but there is still a need to expand the sample size further confirmed that.2 and SIRT1 gene rs7895833 polymorphism and systolic blood pressure levels, rs2273773 polymorphism is related to the level of HDLC, SIRT1 and rs7895833 gene rs2273773, rs7069102 polymorphism and hypertensive cerebral hemorrhage group dysfunction (NIHSS score) may have nothing to do.

【學(xué)位授予單位】：福建中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R544.1;R743.34

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