本文關(guān)鍵詞： 心肌梗塞 臍帶 間質(zhì)干細(xì)胞移植 粒細(xì)胞巨噬細(xì)胞集落刺激因子 轉(zhuǎn)化生長因子β 組織工程 干細(xì)胞 移植 人臍帶間充質(zhì)干細(xì)胞 心肌梗死 粒細(xì)胞-巨噬細(xì)胞集落刺激因子 心功能　出處：《中國組織工程研究》2017年25期 　論文類型：期刊論文

【摘要】：背景:人臍帶間充質(zhì)干細(xì)胞具有分化潛力大,增殖能力強,免疫原性較低等特點,在創(chuàng)傷修復(fù)、局部缺血組織的血管再生等方面發(fā)揮不可估量的作用。目的:探討心室內(nèi)注射人臍帶間充質(zhì)干細(xì)胞對心肌梗死大鼠心肌組織粒細(xì)胞-巨噬細(xì)胞集落刺激因子、轉(zhuǎn)化生長因子β1表達(dá)的影響。方法:選取成年雄性健康SD大鼠120只,隨機選取20只為健康對照組,余100只于腹腔皮下多點注射異丙腎上腺素建立心肌梗死大鼠模型。造模成功后隨機分為模型組、人臍帶間充質(zhì)干細(xì)胞上清液組、人臍帶間充質(zhì)干細(xì)胞低劑量組、中劑量組及高劑量組,每組20只。造模后24 h,分別將生理鹽水注射液、人臍帶間充質(zhì)干細(xì)胞上清液、含0.25×10~6個,1.0×10~6個,4.0×10~6個人臍帶間充質(zhì)干細(xì)胞懸液2 mL緩慢注入心室。治療2周后應(yīng)用心臟超聲測定左室射血分?jǐn)?shù)、收縮末期左室容積及舒張末期左室容積。ELISA法測定血清乳酸脫氨酶、肌酸激酶及腦鈉肽前體水平。取心臟標(biāo)本,硝基四氮唑藍(lán)染色法測定心肌梗死面積,Western blot法測定心肌組織粒細(xì)胞-巨噬細(xì)胞集落刺激因子、轉(zhuǎn)化生長因子β1表達(dá)。結(jié)果與結(jié)論:(1)人臍帶間充質(zhì)干細(xì)胞低、中、高劑量組左室射血分?jǐn)?shù)高于模型組及人臍帶間充質(zhì)干細(xì)胞上清液組,而收縮末期左室容積、舒張末期左室容積低于模型組及人臍帶間充質(zhì)干細(xì)胞上清液組(P0.05),人臍帶間充質(zhì)干細(xì)胞中、高劑量組上述指標(biāo)優(yōu)于低劑量組(P0.05);(2)人臍帶間充質(zhì)干細(xì)胞低、中、高劑量組血清乳酸脫氨酶、肌酸激酶及腦鈉肽前體水平及心肌組織粒細(xì)胞-巨噬細(xì)胞集落刺激因子、轉(zhuǎn)化生長因子β1表達(dá)均低于模型組及人臍帶間充質(zhì)干細(xì)胞上清液組(P0.05),而人臍帶間充質(zhì)干細(xì)胞中、高劑量組上述指標(biāo)低于低劑量組(P0.05);(3)人臍帶間充質(zhì)干細(xì)胞低、中、高劑量組心肌梗死面積小于模型組及人臍帶間充質(zhì)干細(xì)胞上清液組(P0.05),而人臍帶間充質(zhì)干細(xì)胞中、高劑量組心肌梗死面積小于低劑量組(P0.05);(4)結(jié)果表明,人臍帶間充質(zhì)干細(xì)胞能明顯降低心肌梗死大鼠心肌組織粒細(xì)胞-巨噬細(xì)胞集落刺激因子、轉(zhuǎn)化生長因子β1表達(dá),進(jìn)而有效保護(hù)心肌組織,改善心功能。
[Abstract]:Background: human umbilical cord mesenchymal stem cells have the characteristics of great differentiation potential, strong proliferative ability and low immunogenicity. Objective: to investigate the effects of intraventricular injection of human umbilical cord mesenchymal stem cells on myocardial granulocyte-macrophage colony stimulating factor in myocardial infarction rats. Methods: 120 adult male healthy SD rats were selected and 20 healthy rats were randomly selected as healthy control group. The other 100 rats were injected with isoproterenol into the abdominal cavity to establish myocardial infarction model. After the establishment of the model, the rats were randomly divided into model group and human umbilical cord mesenchymal stem cell supernatant group. Human umbilical cord mesenchymal stem cells in low dose group, medium dose group and high dose group, 20 rats in each group. 24 hours after modeling, saline injection, human umbilical cord mesenchymal stem cells supernatant. It contains 0.25 脳 10 ~ 6 ~ 1. 0 脳 10 ~ 6. 4. 0 脳 10 6 human umbilical cord mesenchymal stem cell suspension 2 mL was injected into the ventricle slowly. After 2 weeks of treatment, left ventricular ejection fraction (LVEF) was measured by echocardiography. The serum levels of lactate deaminase, creatine kinase and brain natriuretic peptide precursor were measured by Elisa and left ventricular volume at the end of systolic and diastolic period respectively. Myocardial infarction area was determined by nitrotetrazolium blue staining and the granulocyte-macrophage colony stimulating factor was determined by blot. Results and conclusion Human umbilical cord mesenchymal stem cells were low, and the left ventricular ejection fraction in high dose group was higher than that in model group and human umbilical cord mesenchymal stem cell supernatant group. The left ventricular volume at the end of systolic and diastolic period was lower than that in the model group and the human umbilical cord mesenchymal stem cell supernatant group (P0.05), and in the human umbilical cord mesenchymal stem cells. The above indexes in the high dose group were better than those in the low dose group (P 0.05). The levels of serum lactate deaminase, creatine kinase and brain natriuretic peptide precursor and myocardial granulocyte-macrophage colony stimulating factor were low in human umbilical cord mesenchymal stem cells. The expression of transforming growth factor 尾 1 was lower than that of model group and human umbilical cord mesenchymal stem cell supernatant group (P0.05), but in human umbilical cord mesenchymal stem cells. The above indexes in the high dose group were lower than those in the low dose group (P 0.05). 3) Human umbilical cord mesenchymal stem cells were low, and the myocardial infarction size in high dose group was smaller than that in model group and human umbilical cord mesenchymal stem cell supernatant group (P0.05), while in human umbilical cord mesenchymal stem cell group. The area of myocardial infarction in high dose group was smaller than that in low dose group (P 0.05). The results showed that human umbilical cord mesenchymal stem cells could significantly reduce the expression of granulocyte-macrophage colony stimulating factor and transforming growth factor 尾 1 in myocardial tissue of rats with myocardial infarction. Improve cardiac function.
【作者單位】： 南陽醫(yī)學(xué)高等�？茖W(xué)校第一附屬醫(yī)院心血管外科;
【分類號】：R542.22
【正文快照】： 0引言Introduction復(fù)受損心肌細(xì)胞的理想細(xì)胞來源[10-11]。為此,實驗探討人心肌梗死導(dǎo)致心肌細(xì)胞凋亡而引起心臟局部浸潤性臍帶間充質(zhì)干細(xì)胞對心肌梗死大鼠心肌功能的改善作用及炎癥反應(yīng),并介導(dǎo)免疫細(xì)胞趨化至炎癥浸潤部位,在清除其作用機制。壞死心肌細(xì)胞同時會大量釋放炎癥

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