本文關(guān)鍵詞： 原發(fā)性高血壓 利鈉肽清除受體基因 單核苷酸多態(tài)性 體重指數(shù)　出處：《寧波大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：目的探討利鈉肽清除受體(natriuretic peptide clearance receptor,NPRC)基因(NPR3)多態(tài)性與原發(fā)性高血壓(essential hypertension,EH)的關(guān)系。方法本研按照病例-對(duì)照方法設(shè)計(jì),收集原發(fā)性高血壓患者組452例和年齡、性別相仿的血壓正常者為對(duì)照組434例作為研究對(duì)象。對(duì)吸煙史、飲酒史、2型糖尿病病史、高血壓病病史和家族史等進(jìn)行記錄。檢測(cè)血生化項(xiàng)目:包括總膽固醇、甘油三酯、低密度脂蛋白膽固醇、高密度脂蛋白膽固醇、空腹血糖、尿酸、肌酐、尿素氮、鈉離子和鉀離子等指標(biāo)。使用標(biāo)簽SNP(Tag SNP)策略選擇9個(gè)NPR3基因SNPs位點(diǎn)(rs3792758、rs1173773、rs13436831、rs11750438、rs1173747、rs2292026、rs976576、rs696831、rs3811953)。采用多聚酶鏈反應(yīng)(polymerase chain reaction,PCR)技術(shù)擴(kuò)增NPR3基因片段DNA,經(jīng)多重SNa Pshot平臺(tái)技術(shù)SNPs基因高效擴(kuò)增,利用ABI 3130基因分析儀分析基因型,運(yùn)用Peak Scanner 1.1軟件分析結(jié)果。NPR3基因SNPs位點(diǎn)基因型在病例和對(duì)照組中的分布頻率使用Hardy-Weinberg平衡檢驗(yàn)。統(tǒng)計(jì)軟件為SPSS18.0軟件,t檢驗(yàn)、卡方檢驗(yàn)和二元Logistic回歸分析用于數(shù)據(jù)統(tǒng)計(jì)分析,雙側(cè)檢驗(yàn),P0.05為具有統(tǒng)計(jì)學(xué)意義。應(yīng)用Haploview4.2程序作連鎖不平衡分析。結(jié)果Hardy-Weinberg平衡檢驗(yàn)采用卡方檢驗(yàn),所選9個(gè)SNPs在病例組和對(duì)照組人群中P值均0.05,符合Hardy-Weinberg平衡定律。病例組與對(duì)照組NPR3基因SNPs基因型經(jīng)二元Logistic回歸分析,rs1173773GG基因型攜帶者患高血壓病的風(fēng)險(xiǎn)增加,OR值95%CI 3.164(1.011-9.903),P值0.037,具有統(tǒng)計(jì)學(xué)意義,經(jīng)調(diào)整年齡、性別、體重指數(shù)、腰圍、吸煙史、飲酒史和高血壓病家族史后,OR值95%CI 3.012(1.002-9.728),P值0.041,仍具有統(tǒng)計(jì)學(xué)意義。其他8個(gè)SNPs在兩組之間比較未發(fā)現(xiàn)患高血壓病風(fēng)險(xiǎn),P值均0.05。兩組等位基因型比較經(jīng)卡方檢驗(yàn),P值均0.05,差異無統(tǒng)計(jì)學(xué)意義。進(jìn)一步在高血壓病組內(nèi)比較,BMI"g25Kg/m2與BMI25Kg/m2相比,GG基因型攜帶者超重或者肥胖的風(fēng)險(xiǎn)更高,OR值95%CI3.509(0.935-13.176),P值0.049,具有統(tǒng)計(jì)學(xué)意義;G/A等位基因型攜帶比較,P0.05,無統(tǒng)計(jì)學(xué)意義。另外,在高血壓病組內(nèi)是否合并2型糖尿病各113名比較,G/A等位基因型攜帶分布差異無統(tǒng)計(jì)學(xué)意義。結(jié)論本研究發(fā)現(xiàn)在中國(guó)漢族人群中,NPR3基因rs1173773 GG基因攜帶者患原發(fā)性高血壓風(fēng)險(xiǎn)增加;且原發(fā)性高血壓合并超重或肥胖的風(fēng)險(xiǎn)亦增加。
[Abstract]:Objective to investigate natriuretic peptide clearance receptor. NPRC3) polymorphism was associated with essential hypertensionin essential hypertension (EH). Methods the study was designed with a case-control method. A total of 452 patients with essential hypertension and patients with normal blood pressure of similar age and sex were collected as control group 434 cases as control group. The history of smoking and drinking were compared with the history of type 2 diabetes mellitus. The history of hypertension and family history were recorded. The blood biochemical items included total cholesterol, triglyceride, low density lipoprotein cholesterol, high density lipoprotein cholesterol, fasting blood glucose, uric acid, creatinine. Urea nitrogen, sodium ion and potassium ion were used to select 9 SNPs loci of NPR3 gene, rs3792758 and rs1173773. Rs13436831,rs11750438,rs1173747,rs2292026,rs976576,rs696831. NPR3 gene fragment DNA was amplified by polymerase chain reaction (PCR) and polymerase chain reaction- PCR (PCR) technique. The SNPs gene was amplified efficiently by multiplex SNa Pshot platform, and genotypes were analyzed by ABI 3130 gene analyzer. Using Peak Scanner. 1.1 results of software analysis. The distribution frequency of SNPs locus genotype of NPR3 gene in case and control group was tested by Hardy-Weinberg equilibrium test. The statistical software was SPSS18. .0 software. T test, chi-square test and binary Logistic regression analysis were used for statistical analysis and bilateral test. P0.05 was statistically significant. Haploview4.2 program was used for linkage disequilibrium analysis. Results the Hardy-Weinberg balance test was chi-square test. The 9 SNPs were all P 0. 05 in the case group and the control group. The SNPs genotype of NPR3 gene in case group and control group was analyzed by binary Logistic regression analysis. The risk of hypertension in rs1173773GG genotype carriers was increased by 95 CI 3.164U 1.011-9.903 P value 0.037. After adjusting for age, sex, body mass index, waist circumference, smoking history, drinking history and family history of hypertension, OR was 95 CI 3.0121.002-9.728). P value was 0. 041, which was still statistically significant. The other 8 SNPs were not found in the two groups. The allele genotypes of the two groups were compared with each other by chi-square test. There was no significant difference in P value (P = 0.05). Further comparison was made between BMI "g _ 25kg / m ~ 2 and BMI25Kg/m2 in hypertension group." The risk of overweight or obesity in GG genotype carriers was higher than that in GG genotype carriers. The OR value of GG genotype carriers was 95CI3.509 ~ 0.935-13.176 (P = 0.049), which was statistically significant. There was no significant difference in the G / A allele carrying rate (P 0.05). In addition, 113 patients with type 2 diabetes were compared in the hypertension group whether or not they were associated with type 2 diabetes. There was no significant difference in the distribution of G / A allele genotypes. Conclusion this study found that G- A alleles were found in Chinese Han population. The risk of essential hypertension was increased in NPR3 rs1173773 GG carriers. The risk of essential hypertension associated with overweight or obesity also increased.
【學(xué)位授予單位】：寧波大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R544.11

【共引文獻(xiàn)】

相關(guān)期刊論文 前6條

1 史偉文;吳永貴;沈裕欣;;101例腹膜透析患者心功能評(píng)價(jià)及意義[J];安徽醫(yī)學(xué);2013年11期

2 白文樓;姜志安;王濤;殷洪山;;早期應(yīng)用重組人腦利鈉肽對(duì)急性ST段抬高型廣泛前壁心肌梗死患者PCI術(shù)后心臟保護(hù)作用[J];河北醫(yī)科大學(xué)學(xué)報(bào);2015年04期

3 王輝波;楊俊;彭娟;;LCZ696在慢性心力衰竭中的研究進(jìn)展[J];臨床心血管病雜志;2015年05期

4 孫麗秀;支繼新;高宇囡;劉俊艷;李學(xué)奇;;rhBNP對(duì)心力衰竭合并腎功能不全的臨床療效觀察[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2013年25期

5 朱旖;徐慧蓮;周衛(wèi)華;;淺談?dòng)枚蜇惿程怪委熇夏晷牧λソ叩呐R床效果[J];當(dāng)代醫(yī)藥論叢;2014年14期

6 陳維明;姚亞軍;梁麗;施益忠;侯杰;;凝血功能變化與冠心病近期預(yù)后的關(guān)系[J];中華全科醫(yī)學(xué);2015年09期

相關(guān)博士學(xué)位論文 前3條

1 劉惠良;心臟收縮力調(diào)節(jié)對(duì)慢性心力衰竭兔心功能影響及其機(jī)制研究[D];河北醫(yī)科大學(xué);2014年

2 茍文鈺;雞胚胎低氧適應(yīng)表型及心臟組織差異表達(dá)基因鑒定[D];中國(guó)農(nóng)業(yè)大學(xué);2015年

3 王鵬博;重組人心鈉肽治療心力衰竭的Ⅲ期臨床試驗(yàn)[D];北京協(xié)和醫(yī)學(xué)院;2015年

相關(guān)碩士學(xué)位論文 前4條

1 洪冰;慢性心衰合并房顫患者血漿腦鈉肽的變化及意義[D];天津醫(yī)科大學(xué);2013年

2 白文樓;早期應(yīng)用重組人腦利鈉肽對(duì)急性ST段抬高型廣泛前壁心�；颊逷CI術(shù)后心臟保護(hù)作用[D];河北醫(yī)科大學(xué);2014年

3 馬海娥;血漿BNP結(jié)合心率變異性對(duì)高血壓患者危險(xiǎn)分層的臨床研究[D];延安大學(xué);2014年

4 陳麗華;原發(fā)性高血壓患者血漿D型利鈉肽（DNP）的水平及意義[D];福建醫(yī)科大學(xué);2014年

，

本文編號(hào)：1462825