谷胱甘肽巰基轉(zhuǎn)移酶α1/α4(GSTA1/A4)是體內(nèi)重要的解毒酶,可降低多種內(nèi)、外源性毒性化合物的毒性.然而,膽汁淤積病人肝細(xì)胞內(nèi)G STA1/A4的表達(dá)是下調(diào)的,下調(diào)機(jī)制尚不清楚.本研究通過(guò)腫瘤壞死因子α(TNFα)處理人肝癌細(xì)胞Hep G2細(xì)胞,利用實(shí)時(shí)熒光定量聚合酶鏈?zhǔn)椒磻?yīng)(q PCR)和蛋白質(zhì)印跡(Western blot)檢測(cè)GSTA1/A4、核因子κB(NF-κB)和核因子E2相關(guān)因子2(Nrf2)的表達(dá).發(fā)現(xiàn)TNFα在m RNA水平和蛋白質(zhì)水平均抑制GSTA1/A4表達(dá),且呈劑量與時(shí)間依賴關(guān)系.干擾NF-κB信號(hào)通路,可減弱T NFα對(duì)G STA1/A4表達(dá)的抑制作用.以上結(jié)果表明,在Hep G2細(xì)胞中,TNFα可通過(guò)激活N F-κB信號(hào)通路抑制G STA1/A4表達(dá).

【文章頁(yè)數(shù)】：9 頁(yè)

【文章目錄】：
1 Materials and methods
    1.1 Animal studies
    1.2 Hep G2 cells culture and treatment
    1.3 Cell viability analysis
    1.4RNA extraction and quantitative real-time polymerase chain reaction(q PCR)
    1.5Protein extraction and Western blotting analysis
    1.6 Statistical analysis
2 Results
    2.1 Reduction of Gst levels in the liver of BDL rats
    2.2 The bile acid content did not correlate with GSTA1 and GSTA4 expression in Hep G2 cells
    2.3 GSTA1 and GSTA4 expression in Hep G2cells was repressed by TNFα
    2.4 TNFαactivated NF-κB signaling and did not repress Nrf2 protein expression
    2.5 Inhibition of NF-κB activation attenuated TNFα-mediated decrease in GSTA1 and GSTA4expression
3 Discussion



本文編號(hào)：3998391