目的:腹瀉型腸易激綜合征（diarrhea irritable bowel syndrome,IBS-D）是一種臨床常見的功能性腸病,這種疾病的高發(fā)病率嚴(yán)重影響患者的生活質(zhì)量,對衛(wèi)生醫(yī)療資源造成很大的壓力。IBS-D的病理生理學(xué)機制較為復(fù)雜。目前,其發(fā)病機制尚不明確。IBS-D患者常存在明顯的胃腸動力異常,表現(xiàn)出輕度或者嚴(yán)重的腹瀉并伴隨出現(xiàn)腹痛等癥狀。研究發(fā)現(xiàn)腸道屏障功能的破壞以及腸道微生態(tài)的改變是其發(fā)病的重要原因。本實驗旨在探討IBS-D大鼠腸道微生態(tài)的組成改變以及與腸道屏障相關(guān)的緊密連接蛋白的變化,為IBS-D發(fā)病機制提供實驗數(shù)據(jù)和理論基礎(chǔ)。實驗方法:將Wistar雄性大鼠隨機分為對照組和模型組,對大鼠進行胃腸電極埋置手術(shù),分別于胃竇、十二指腸、結(jié)腸部位埋置電極,檢測兩組大鼠胃腸肌電活動的改變。除此之外,對胃腸動力檢測還應(yīng)用了胃排空腸推進和結(jié)腸排珠實驗;模型評價還包括腹壁撤退反射評分（abdominal withdrawal reflex,AWR）實驗、HE染色、大鼠糞便含水量的檢測;采用ELISA技術(shù)檢測血漿中D-乳酸的含量;通過曠場實驗以及糖水偏嗜實驗評價IBS-D大鼠的焦慮抑... 

【文章來源】：蘭州大學(xué)甘肅省 211工程院校 985工程院校 教育部直屬院校

【文章頁數(shù)】：57 頁

【學(xué)位級別】：碩士

【部分圖文】：

腹瀉型腸易激綜合征大鼠胃腸動力的改變(a)IntestinalpropulsionandgastricemptyinginIBS-Drats.(b)ColonicbeadinginIBS-Drats.

蘭州大學(xué)碩士學(xué)位論文腹瀉型腸易激綜合征大鼠腸道菌群和腸道屏障功能的變化及相關(guān)分子機制的研究24和細(xì)胞質(zhì)染色，模型組結(jié)腸AQP3蛋白表達水平明顯低于對照組。Figure6.ChangesofaquaporinsincolonofIBS-Drats.圖6.腹瀉型腸易激綜合征大鼠結(jié)腸部位水通道蛋白的改變(a)ChangesofAQP3expressionincolon.(b)ChangesofAQP8expressionincolon.(c)ImmunohistochemistryforAQP3proteininthecolon(magnified100×scarbars:100μm;200×scarbars:50μm;and400×scarbars:50μm).Comparedtocontrol:*P<0.05,**P<0.01,***P<0.001.

蘭州大學(xué)碩士學(xué)位論文腹瀉型腸易激綜合征大鼠腸道菌群和腸道屏障功能的變化及相關(guān)分子機制的研究253.7IBS-D緊密連接蛋白的改變qPCR和WesternBlot實驗結(jié)果顯示，與對照組相比IBS-D大鼠結(jié)腸組織中occludinmRNA以及蛋白質(zhì)表達明顯降低（P<0.01）,claudin-4mRNA以及蛋白質(zhì)表達明顯升高（P<0.05），模型組ZO-1mRNA的表達也顯著降低（P<0.05）（Figure7.a,b,c,d,e）。免疫熒光染色顯示claudin-4分布于腸上皮（包括細(xì)胞膜頂部）緊密結(jié)合的細(xì)胞膜中，模型組結(jié)腸中claudin-4熒光強度更高（Figure7.f）。Figure7.ChangesofTJproteinsinIBS-Drats.圖7.腹瀉型腸易激綜合征大鼠緊密連接蛋白的改變(a)ThelevelofoccludinmRNAinhomogenatesofthecolon.(b)Thelevelofoccludinproteininhomogenatesofthecolon.(c)Thelevelofclaudin-4mRNAinthecolon.(d)Thelevelofclaudin-4proteininthecolon.(e)ThelevelofZO-1mRNAinhomogenatesofthecolon.(f)Immunofluorescencestainingdemonstratingthelocalizationofclaudin-4(red)(100×scarbars:100μm,200×scarbars:50μm,400×scarbars:50μm).Statisticalsignificancewasdeterminedcomparedtothecontrolgroup,*P<0.05,**P<0.01.

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