【摘要】：目的觀察煙堿和迷走神經(jīng)切斷對(duì)大鼠重癥急性胰腺炎(SAP)肺損傷的影響。方法 SPF級(jí)雌性Wistar大鼠48只,按隨機(jī)數(shù)字表法分為6組,8只/組,分別予SAP造模假手術(shù)+生理鹽水治療(Sham+saline組),SAP+生理鹽水治療(SAP+saline組),SAP+煙堿治療(SAP+nicotine組);正常對(duì)照大鼠+迷走神經(jīng)切斷假手術(shù)(Con+sham surgery組),SAP+迷走神經(jīng)切斷假手術(shù)(SAP+sham surgery組),SAP+迷走神經(jīng)切斷(SAP+vagotomy組)。采用膽胰管逆行注射5%�；悄懰徕c建立大鼠SAP模型,術(shù)前30 min腹腔注射煙堿,或術(shù)前3 d行左側(cè)迷走神經(jīng)切斷。術(shù)后12 h剖殺大鼠,觀察胰腺、肺組織病理學(xué)變化,檢測(cè)血清淀粉酶(AMY)、脂肪酶(LIPA),酶聯(lián)免疫吸附法(ELISA)檢測(cè)血清白細(xì)胞介素-6(IL-6),試劑盒測(cè)定肺組織髓過氧化物酶(MPO),免疫印跡法(Western blot)檢測(cè)肺組織高遷移率族蛋白B1(HMGB1)。結(jié)果 SAP+saline組胰腺、肺組織病理學(xué)評(píng)分,AMY、LIPA、IL-6、MPO和HMGB1均較Sham+saline組升高(P0.05),SAP+nicotine組上述指標(biāo)均較SAP+saline組下降(P0.05),SAP+sham surgery組上述指標(biāo)均較Con+sham surgery組升高(P0.05),SAP+vagotomy組上述指標(biāo)均較SAP+sham surgery組升高(P0.05)。結(jié)論煙堿可通過下調(diào)IL-6、MPO和HMGB1減輕SAP肺損傷,單側(cè)迷走神經(jīng)切斷可加劇SAP肺損傷。
[Abstract]:Objective to observe the effects of nicotine and vagus nerve transection on (SAP) lung injury in rats with severe acute pancreatitis (SAP). Methods 48 SPF female Wistar rats were randomly divided into 6 groups, 8 rats / group, treated with), SAP saline in (Sham saline group and), SAP nicotine group in (SAP saline group, respectively, and normal control rats were treated with), SAP vagus nerve transection in (Con sham surgery group and), SAP vagus nerve transection (SAP vagotomy group in (SAP sham surgery group. The rat model of SAP was established by retrograde injection of 5% sodium taurocholate into the cholancreatic duct. Nicotine was injected intraperitoneally 30 min before operation or left vagus nerve was cut off 3 days before operation. Rats were killed 12 hours after operation. Pathological changes of pancreas and lung tissue were observed, serum amylase (AMY), lipase (LIPA), enzyme linked immunosorbent assay (Elisa) (ELISA) was used to detect serum IL-6 (IL-6), and lung tissue myeloperoxidase (MPO), immunoblotting (Western blot) was used to detect high mobility group protein B1 (HMGB1) in lung tissue. Results the pathological scores of pancreas and lung tissue, AMY,LIPA,IL-6,MPO and HMGB1 in SAP saline group were higher than those in Sham saline group (P 0.05), and the above indexes in), SAP nicotine group were lower than those in SAP saline group (P 0.05). The above indexes in), SAP sham surgery group were higher than those in Con sham surgery group (P 0.05), and the above indexes in), SAP vagotomy group were higher than those in SAP sham surgery group (P 0.05). Conclusion nicotine can reduce lung injury in SAP by down-regulating IL-6,MPO and HMGB1, and unilateral vagotomy can aggravate lung injury in SAP.
【作者單位】： 南方醫(yī)科大學(xué)附屬南海醫(yī)院消化內(nèi)科;
【基金】：廣東省佛山市醫(yī)學(xué)類科技攻關(guān)項(xiàng)目(編號(hào):2014AB000132)
【分類號(hào)】：R576;R-332

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