【摘要】：目的觀察二氫楊梅素(dihydromyricetin,DHM)治療潰瘍性結(jié)腸炎(ulcerative colitis,UC)的效果并探討其機(jī)制。方法 60只BALB/c小鼠采用隨機(jī)數(shù)字表法分為正常組,模型組,DHM低劑量[50 mg/(kg·d)]、中劑量[100 mg/(kg·d)]、高劑量[200 mg/(kg·d)]組,5-氨基水楊酸[5-amino saliciylic acid,5-ASA,50 mg/(kg·d)]組。分組當(dāng)日給予正常組小鼠蒸餾水自行飲用,其余組小鼠給予4%葡聚糖硫酸鈉(DSS)水溶液7 d誘導(dǎo)急性UC模型。于飲用DSS當(dāng)日開始,用DHM各劑量組及5-氨基水楊酸組按各組擬定劑量分別進(jìn)行灌胃處理,連續(xù)給藥14 d。對比各組疾病活動指數(shù)(DAI)、結(jié)腸長度、脾臟指數(shù)的變化、結(jié)腸組織學(xué)評分,腸組織TLR2、TLR4和NF-κB的活性以及炎性細(xì)胞因子IL-1β、TNF-α、IL-6表達(dá)及MPO、NO、SOD的活性變化。結(jié)果與模型組比較,DHM可顯著降低結(jié)腸組織中TLR2、TLR4、NF-κB的表達(dá),減少炎性因子如IL-1β、TNF-α、IL-6水平,同時(shí)還能夠減少腸組織中MPO、NO活性及增加SOD的活性。其中DHM高劑量組優(yōu)于低、中劑量組,差異有統(tǒng)計(jì)學(xué)意義(P0.05),治療效果最明顯,其與5-氨基水楊酸組相比差異無統(tǒng)計(jì)學(xué)意義(P0.05),治療效果相當(dāng)。結(jié)論 DHM可顯著緩解DSS誘導(dǎo)的小鼠結(jié)腸炎癥,其作用機(jī)制可能與抗炎、抗氧化有關(guān)。
[Abstract]:Objective To observe the effect of dihydromyricetin (DHM) on ulcerative colitis (UC) and its mechanism. Methods 60 BALB/ c mice were divided into normal group, model group, DHM low dose[50 mg/ (kg 路 d)], middle dose[100 mg/ (kg 路 d)], high dose[200 mg/ (kg 路 d)] and 5-aminosalicylic acid[5-amino salicylic acid,5-ASA,50 mg/ (kg 路 d)]. The normal group of mice were given the normal group of distilled water for self-drinking on the same day, and the rest of the mice were given 4% sodium dextran sulfate (DSS) aqueous solution for 7 days to induce the acute UC model. At the beginning of the day of drinking, the dosage groups of DHM and 5-aminosalicylic acid were administered intragastric respectively for 14 days. The changes of the activity index (DAI), the length of the colon, the index of the spleen, the histological grade of the colon, and the intestinal tissue TLR2 were compared. The activity of TLR4 and NF-EMAB and the activity of IL-1, TNF-1, IL-6 and MPO, NO, and SOD of inflammatory cytokines. Results Compared with the model group, DHM can significantly lower the expression of TLR2, TLR4, NF-B in the colon tissue, reduce the level of the inflammatory factors such as IL-1, TNF-1, and IL-6, and also reduce the activity of MPO and NO in the intestinal tissue and increase the activity of SOD. The results showed that the high dose group of DHM was superior to that of the low and middle dose group (P0.05). The treatment effect was the most obvious. The difference of the treatment effect was not significant (P0.05), and the treatment effect was comparable. Conclusion DHM can alleviate the colonic inflammation of mice induced by DSS, and its mechanism of action may be related to anti-inflammatory and anti-oxidation.
【作者單位】： 蘭州大學(xué)第二醫(yī)院消化科;甘肅省人民醫(yī)院西院消化科;甘肅省消化系腫瘤重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金面上項(xiàng)目(81272661) 甘肅省自然科學(xué)基金(1506RJZA204)~~
【分類號】：R574.62

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