炎癥性腸病患者外周血IL-12家族細胞因子的表達及臨床意義
發(fā)布時間：2019-03-18 10:00
【摘要】：炎癥性腸病(IBD)是一組主要以腸道慢性炎癥反應為表現(xiàn)的疾病,免疫異常被認為在疾病發(fā)病過程中具有非常重要的作用,其中細胞因子參與了腸道免疫反應與炎癥進程。本研究通過檢測對比98例IBD患者及24例腸息肉患者血清IL-12家族細胞因子含量,探討其在炎癥性腸病發(fā)病過程中的作用,為指導臨床IBD的治療提供新思路。目的探討白介素12(IL-12)家族細胞因子包括IL-12、IL-23、IL-27及IL-35在炎癥性腸病(inflammatory bowel disease)患者血清中的表達情況及其臨床意義。方法經(jīng)過數(shù)據(jù)質(zhì)量處理,98例確診為炎癥性腸病(inflammatory bowel diseases,IBD)患者的外周血作為疾病組(其中克羅恩病61例,潰瘍性結(jié)腸炎37例),另采集24例腸息肉患者的外周血作為對照組,利用酶聯(lián)免疫吸附法(ELISA)檢測疾病組與對照組血清中IL-12、IL-23、IL-27及IL-35的含量,比較各組細胞因子表達水平差異并分析其與疾病特征之間的關(guān)系。結(jié)果潰瘍性結(jié)腸炎患者血清中IL-12、IL-23、IL-27的表達水平顯著高于對照組(p值分別為0.001、0.001、0.023),IL-35的表達水平低于對照組(p值為0.001);克羅恩病患者血清中IL-12、IL-23、IL-27的表達水平均顯著高于對照組,(p值分別為0.001、0.001、0.003),IL-35的表達水平低于對照組(p值為0.001);相關(guān)性分析顯示IL-12、IL-23、IL-35血清表達水平與炎癥性腸病(IBD)發(fā)病相關(guān),(OR=1.2501,P=0.0407;OR=1.9554,P=0.0101;OR=0.92043,P=0.0139)。IL-12、IL-23及IL-35血清表達水平與潰瘍性結(jié)腸炎的發(fā)病相關(guān)(OR=1.2490,P=0.0411;OR=1.9413,P=0.0124,OR=0.9159,P=0.0147),IL-12、IL-27及IL-35血清表達水平與克羅恩病的發(fā)病相關(guān)(OR=1.3964,P=0.0079;OR=1.0949,P=0.0469,OR=0.9408,P=0.0107);IL-12血清表達水平與克羅恩病以及潰瘍性結(jié)腸炎病情的嚴重程度呈正相關(guān)(P0.001;P=0.0398),而其他細胞因子與克羅恩病、潰瘍性結(jié)腸炎疾病嚴重程度間均無關(guān)聯(lián)。結(jié)論IL-12家族細胞的血清表達均與炎癥性腸病的發(fā)病關(guān)系密切,除IL-12外,其它細胞因子與炎癥性腸病嚴重程度無顯著關(guān)聯(lián)。
[Abstract]:Inflammatory bowel disease (IBD) is a group of diseases characterized by chronic intestinal inflammation. Immune abnormalities are considered to play a very important role in the pathogenesis of inflammatory bowel diseases, in which cytokines play an important role in intestinal immune response and inflammation. In this study, the levels of serum IL-12 family cytokines in 98 patients with IBD and 24 patients with intestinal polyps were measured and compared to explore the role of cytokines in the pathogenesis of inflammatory bowel disease, and provide a new way to guide the treatment of clinical IBD. Objective to investigate the expression of interleukin-12 (IL-12) family cytokines including IL-12,IL-23,IL-27 and IL-35 in the serum of patients with inflammatory bowel disease (inflammatory bowel disease) and its clinical significance. Methods after data quality treatment, 98 patients with inflammatory bowel disease (inflammatory bowel diseases,IBD) were treated as the disease group (including 61 Crohn's disease and 37 ulcerative colitis), and all the patients were divided into two groups: Crohn's disease (61 cases) and ulcerative colitis (37 cases). The peripheral blood of 24 patients with intestinal polyps was collected as the control group. The levels of IL-12,IL-23,IL-27 and IL-35 in serum were detected by enzyme-linked immunosorbent assay (ELISA) in the patients with intestinal polyps and those in the control group. The expression level of cytokines in each group was compared and the relationship between cytokine expression and disease characteristics was analyzed. Results the expression of IL-12,IL-23,IL-27 in the serum of patients with ulcerative colitis was significantly higher than that in the control group (p = 0.001, 0.001, 0.023, respectively), and the expression level of IL-35 was lower than that in the control group (p = 0.001). The expression of IL-12,IL-23,IL-27 in serum of patients with Crohn's disease was significantly higher than that in control group (p = 0.001, 0.001, 0.003, respectively), and the expression level of IL-35 was lower than that of control group (p = 0.001). Correlation analysis showed that serum expression of IL-12,IL-23,IL-35 was associated with the pathogenesis of (IBD) in inflammatory bowel disease (OR=1.2501,P=0.0407;OR=1.9554,P=0.0101;). The expression of IL-12,IL-23 and IL-35 in serum was associated with the pathogenesis of ulcerative colitis (OR=1.2490,P=0.0411;). OR=0.92043,P=0.0139). The serum levels of OR=1.9413,P=0.0124,OR=0.9159,P=0.0147, IL-12,IL-27 and IL-35 were correlated with the pathogenesis of Crohn's disease (OR=1.3964,P=0.0079;OR=1.0949,P=0.0469,OR=0.9408,P=0.0107). The serum expression of IL-12 was positively correlated with the severity of Crohn's disease and ulcerative colitis (P0.001), but no correlation was found between other cytokines and the severity of Crohn's disease and ulcerative colitis. Conclusion the serum expression of IL-12 family cells is closely related to the pathogenesis of inflammatory bowel disease, except for IL-12, there is no significant correlation between other cytokines and the severity of inflammatory bowel disease.
【學位授予單位】：上海交通大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R574.62
本文編號：2442751
[Abstract]:Inflammatory bowel disease (IBD) is a group of diseases characterized by chronic intestinal inflammation. Immune abnormalities are considered to play a very important role in the pathogenesis of inflammatory bowel diseases, in which cytokines play an important role in intestinal immune response and inflammation. In this study, the levels of serum IL-12 family cytokines in 98 patients with IBD and 24 patients with intestinal polyps were measured and compared to explore the role of cytokines in the pathogenesis of inflammatory bowel disease, and provide a new way to guide the treatment of clinical IBD. Objective to investigate the expression of interleukin-12 (IL-12) family cytokines including IL-12,IL-23,IL-27 and IL-35 in the serum of patients with inflammatory bowel disease (inflammatory bowel disease) and its clinical significance. Methods after data quality treatment, 98 patients with inflammatory bowel disease (inflammatory bowel diseases,IBD) were treated as the disease group (including 61 Crohn's disease and 37 ulcerative colitis), and all the patients were divided into two groups: Crohn's disease (61 cases) and ulcerative colitis (37 cases). The peripheral blood of 24 patients with intestinal polyps was collected as the control group. The levels of IL-12,IL-23,IL-27 and IL-35 in serum were detected by enzyme-linked immunosorbent assay (ELISA) in the patients with intestinal polyps and those in the control group. The expression level of cytokines in each group was compared and the relationship between cytokine expression and disease characteristics was analyzed. Results the expression of IL-12,IL-23,IL-27 in the serum of patients with ulcerative colitis was significantly higher than that in the control group (p = 0.001, 0.001, 0.023, respectively), and the expression level of IL-35 was lower than that in the control group (p = 0.001). The expression of IL-12,IL-23,IL-27 in serum of patients with Crohn's disease was significantly higher than that in control group (p = 0.001, 0.001, 0.003, respectively), and the expression level of IL-35 was lower than that of control group (p = 0.001). Correlation analysis showed that serum expression of IL-12,IL-23,IL-35 was associated with the pathogenesis of (IBD) in inflammatory bowel disease (OR=1.2501,P=0.0407;OR=1.9554,P=0.0101;). The expression of IL-12,IL-23 and IL-35 in serum was associated with the pathogenesis of ulcerative colitis (OR=1.2490,P=0.0411;). OR=0.92043,P=0.0139). The serum levels of OR=1.9413,P=0.0124,OR=0.9159,P=0.0147, IL-12,IL-27 and IL-35 were correlated with the pathogenesis of Crohn's disease (OR=1.3964,P=0.0079;OR=1.0949,P=0.0469,OR=0.9408,P=0.0107). The serum expression of IL-12 was positively correlated with the severity of Crohn's disease and ulcerative colitis (P0.001), but no correlation was found between other cytokines and the severity of Crohn's disease and ulcerative colitis. Conclusion the serum expression of IL-12 family cells is closely related to the pathogenesis of inflammatory bowel disease, except for IL-12, there is no significant correlation between other cytokines and the severity of inflammatory bowel disease.
【學位授予單位】：上海交通大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R574.62
【參考文獻】
相關(guān)期刊論文 前1條
1 白愛平;;炎癥性腸病發(fā)病機制的微生物因素[J];世界華人消化雜志;2006年07期
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