【摘要】：微環(huán)境在胃癌發(fā)病過(guò)程中發(fā)揮重要作用。了解胃粘膜早期癌變的分子機(jī)制,對(duì)防治胃癌具有十分重要的意義。為了解胃粘膜非典型增生過(guò)程中,微環(huán)境中蛋白質(zhì)的相互作用及調(diào)節(jié)機(jī)制,采用激光捕獲顯微切割(laser capture microdissection,LCM)技術(shù),純化正常胃粘膜組織(normal gastric mucosa tissue,NGM)和胃粘膜非典型增生(gastric mucosal atypical hyperplasia,GMAH)間質(zhì),通過(guò)同位素標(biāo)記定量蛋白質(zhì)組學(xué)技術(shù)分析,鑒定NGM和GMAH間質(zhì)的差異表達(dá)蛋白質(zhì)。利用生物信息學(xué)軟件,分析NGM和GMAH間質(zhì)差異表達(dá)蛋白質(zhì)的相互作用及其聯(lián)系。共鑒定出165個(gè)GMAH間質(zhì)差異表達(dá)蛋白質(zhì),其中GMAH組織中表達(dá)上調(diào)者99個(gè),下調(diào)者66個(gè)。它們涉及一些與腫瘤相關(guān)的信號(hào)通路,如p53信號(hào)通路、MAPK信號(hào)通路、細(xì)胞周期與凋亡等信號(hào)通路,且與細(xì)胞生長(zhǎng)、增殖、凋亡和體液免疫應(yīng)答等生物學(xué)過(guò)程有關(guān)。這些差異表達(dá)蛋白質(zhì),在STRING網(wǎng)絡(luò)中呈現(xiàn)相互作用,兩兩間相互聯(lián)系。本文的研究提示,胃粘膜非典型增生微環(huán)境中存在S100A6和SOD3等蛋白質(zhì)間的相互作用,它們通過(guò)影響p53信號(hào)通路、MAPK信號(hào)通路、細(xì)胞周期與凋亡等信號(hào)通路,在胃癌發(fā)病過(guò)程中發(fā)揮作用。
[Abstract]:Microenvironment plays an important role in the pathogenesis of gastric cancer. It is very important to understand the molecular mechanism of early canceration of gastric mucosa in prevention and treatment of gastric cancer. In order to understand the interaction and regulation mechanism of protein in microenvironment during atypical hyperplasia of gastric mucosa, the (normal gastric mucosa tissue, of normal gastric mucosa was purified by laser capture microdissection (laser capture microdissection,LCM) technique. NGM) and (gastric mucosal atypical hyperplasia,GMAH) were used to identify the differentially expressed proteins between NGM and GMAH. The interaction and relationship of differentially expressed proteins between NGM and GMAH were analyzed by bioinformatics software. A total of 165 differentially expressed GMAH proteins were identified, of which 99 were up-regulated in GMAH and 66 were down-regulated. They involve some signal pathways related to tumor, such as p53 signaling pathway, MAPK signaling pathway, cell cycle and apoptosis signaling pathway, and also related to cell growth, proliferation, apoptosis and humoral immune response. These differentially expressed proteins, which interact in STRING networks, are related to each other. The present study suggests that there are interactions between S100A6 and SOD3 in gastric mucosal dysplasia microenvironment, which affect p53 signaling pathway, MAPK signal pathway, cell cycle and apoptosis signaling pathway. Play a role in the pathogenesis of gastric cancer.
【作者單位】： 南華大學(xué)醫(yī)學(xué)院腫瘤研究所腫瘤細(xì)胞與分子病理學(xué)湖南省重點(diǎn)實(shí)驗(yàn)室;南華大學(xué)附屬第一醫(yī)院;邵陽(yáng)醫(yī)學(xué)高等�？茖W(xué)校附屬醫(yī)院兒科;南華大學(xué)醫(yī)學(xué)院;
【基金】：湖南省高校創(chuàng)新平臺(tái)基金(No.12K094,No.13K083) 湖南省科技廳項(xiàng)目(No.2015SK20203) 湖南省教育廳課題(No.11C1112) 湖南省衛(wèi)生廳課題(No.B2013-048,No.2014-163) 湖南省研究生科研創(chuàng)新項(xiàng)目(No.CX2016B478)~~
【分類號(hào)】：R573
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本文編號(hào)：2401860