GnRH、SS及其受體的表達(dá)在顱腦火器傷下急性末端回腸炎中的變化
發(fā)布時(shí)間：2018-11-11 15:19
【摘要】：目的：研究實(shí)驗(yàn)犬在各種應(yīng)激中促性腺激素釋放激素（GnRH）、生長(zhǎng)抑素(SS)濃度的變化及其受體在實(shí)驗(yàn)性急性末端回腸炎中的表達(dá)，初步探討應(yīng)激與激素及其受體的內(nèi)在聯(lián)系，揭示應(yīng)激與腸道疾病的相關(guān)性。 方法：選用健康成年雄性雜種犬40只，隨機(jī)分成8組，每組5只：常溫常濕正常血糖組（A組）、常溫常濕高血糖組（G組）、高溫高濕高血糖組（B組）、高溫高濕正常血糖組（H組）、常溫常濕正常血糖顱腦火器傷組（C組）、常溫常濕高血糖顱腦火器傷組（D組）、高溫高濕正常血糖顱腦火器傷組（E組）、高溫高濕高血糖顱腦火器傷組（F組）。C、D、E、F組分別給予手槍彈顱腦火器傷造模。 造模后立即將犬仰臥位固定于手術(shù)臺(tái)上，并行氣管插管、心電監(jiān)護(hù)、心肺復(fù)蘇等搶救措施，監(jiān)測(cè)傷后心率、血壓等生命體征；除心臟擠壓及人工呼吸氣囊擠壓搶救外，，不做其他處理。在無(wú)菌條件下立即行腹部手術(shù)，剖腹尋找回盲部，切取1-3cm末端回腸組織立即離心保存，然后將殘端吻合。根據(jù)時(shí)間梯度：0小時(shí)、0.5小時(shí)、1小時(shí)、1.5小時(shí)、2小時(shí)，各組實(shí)驗(yàn)犬每只采集外周血5個(gè)樣本，立即離心保存。在此過(guò)程中盡量延緩實(shí)驗(yàn)犬生命，予以靜脈補(bǔ)充液體。采用酶聯(lián)免疫吸附試驗(yàn)(Enzyme-LinkedImmunosorbent Assay,ELISA)定量檢測(cè)外周血中GnRH和SS的濃度變化；采用Westernblot檢測(cè)末端回腸組織中GnRH受體、SS受體在A、B、F組中各時(shí)間點(diǎn)的表達(dá)。 結(jié)果:1．術(shù)后各組實(shí)驗(yàn)犬血漿中GnRH濃度的變化：0h時(shí)8個(gè)實(shí)驗(yàn)組中各實(shí)驗(yàn)犬血漿中GnRH濃度差異不明顯；在0.5h時(shí)各組實(shí)驗(yàn)犬血漿中GnRH濃度明顯下降，考慮與其自身調(diào)節(jié)有關(guān)；隨應(yīng)激后時(shí)間延長(zhǎng)GnRH濃度呈現(xiàn)遞增趨勢(shì)，且明顯超過(guò)起始濃度（0h）； 2．術(shù)后各組實(shí)驗(yàn)犬血漿中SS濃度的變化：0h時(shí)8個(gè)實(shí)驗(yàn)組中各實(shí)驗(yàn)犬血漿中SS濃度差異不明顯；在0.5h時(shí)各組實(shí)驗(yàn)犬血漿中SS濃度明顯下降，考慮與其自身調(diào)節(jié)有關(guān)；隨應(yīng)激后時(shí)間延長(zhǎng)SS濃度呈現(xiàn)遞增趨勢(shì)，且明顯超過(guò)起始濃度（0h）； 3．術(shù)后A、B、F組末端回腸組織GnRH受體、SS受體的表達(dá)變化：采用Westernblot分析末端回腸組織GnRH受體、SS受體的表達(dá)發(fā)現(xiàn)，在急性應(yīng)激狀態(tài)下，GnRHR、SSTR表達(dá)均有增多的趨勢(shì)；且GnRH受體、SS受體與對(duì)應(yīng)GnRH濃度、SS濃度呈現(xiàn)正相關(guān)性。 結(jié)論：1．顱腦火器傷下急性末端回腸炎血漿中GnRH濃度升高及其受體的表達(dá)增多； 2．顱腦火器傷下急性末端回腸炎血漿中SS濃度升高及其受體的表達(dá)增多； 3. GnRH、SS及其受體參與了應(yīng)激對(duì)急性末端回腸炎的調(diào)控，且在一定時(shí)間內(nèi)呈現(xiàn)強(qiáng)度正相關(guān)。
[Abstract]:Objective: to study the changes of gonadotropin releasing hormone (GnRH),) somatostatin (SS) in dogs and the expression of somatostatin (SS) receptor in experimental acute terminal colitis. To explore the relationship between stress and hormones and their receptors, and reveal the relationship between stress and intestinal diseases. Methods: forty healthy adult male mongrel dogs were randomly divided into 8 groups: normal blood glucose group (A group), hyperglycemia group (G group) and hyperglycemia group (B group). Normal blood glucose group (H group), normal blood glucose group (C group), craniocerebral firearm injury group (D group), craniocerebral firearm injury group (E group). The craniocerebral firearm injury group (group F) with high temperature and high humidity and hyperglycemia (group F) were given the model of craniocerebral firearm injury with pistols. The canine supine position was fixed on the operating table immediately after modeling, followed by tracheal intubation, ECG monitoring, cardiopulmonary resuscitation and other rescue measures to monitor the vital signs such as heart rate and blood pressure after injury. No other treatment except cardiac extrusion and artificial respiration sac extrusion. Abdominal surgery was performed immediately under aseptic conditions, ileocecal part was searched by laparotomy, and ileal tissue at the end of 1-3cm was immediately centrifuged and preserved, then the stump was anastomosed. According to the time gradient: 0 hours, 0.5 hours, 1 hour, 1.5 hours, 2 hours, 5 samples of peripheral blood were collected from each group of dogs, and then centrifuged immediately. In the process of delaying the life of experimental dogs as far as possible, intravenous fluid supply. Enzyme linked immunosorbent assay (Enzyme-LinkedImmunosorbent Assay,ELISA) was used to quantitatively detect the concentration of GnRH and SS in peripheral blood, and Westernblot was used to detect the expression of GnRH receptor and SS receptor at different time points in the terminal ileum tissue. Results: 1. The change of plasma GnRH concentration in each group: at 0 h, there was no significant difference in plasma GnRH concentration in 8 experimental groups. At 0.5 h, the concentration of GnRH in plasma of experimental dogs decreased obviously, which was related to their self-regulation, and the concentration of GnRH increased gradually with the prolongation of time after stress, and obviously exceeded the initial concentration (0 h). 2. The changes of plasma SS concentration in each group of experimental dogs: there was no significant difference in plasma SS concentration in each of the eight experimental groups at 0 h, and the SS concentration in plasma of each group decreased significantly at 0.5 h, which was related to its self-regulation. The concentration of SS increased with the prolongation of stress time and exceeded the initial concentration (0 h). 3. The expression of GnRH receptor and SS receptor in the terminal ileum of group A and B: the expression of GnRH receptor and SS receptor in terminal ileum were analyzed by Westernblot, and the expression of GnRHR,SSTR increased under acute stress. There was a positive correlation between GnRH receptor, SS receptor and corresponding GnRH concentration and SS concentration. Conclusion: 1. The concentration of GnRH and the expression of its receptor increased in the plasma of acute terminal colitis after craniocerebral firearm injury, 2. The concentration of SS and the expression of receptor in plasma of acute terminal gynecitis with craniocerebral firearm injury increased; 3. GnRH,SS and its receptors are involved in the regulation of stress on acute terminal colitis, and there is a positive correlation of intensity in a certain period of time.
【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R574
本文編號(hào)：2325261
[Abstract]:Objective: to study the changes of gonadotropin releasing hormone (GnRH),) somatostatin (SS) in dogs and the expression of somatostatin (SS) receptor in experimental acute terminal colitis. To explore the relationship between stress and hormones and their receptors, and reveal the relationship between stress and intestinal diseases. Methods: forty healthy adult male mongrel dogs were randomly divided into 8 groups: normal blood glucose group (A group), hyperglycemia group (G group) and hyperglycemia group (B group). Normal blood glucose group (H group), normal blood glucose group (C group), craniocerebral firearm injury group (D group), craniocerebral firearm injury group (E group). The craniocerebral firearm injury group (group F) with high temperature and high humidity and hyperglycemia (group F) were given the model of craniocerebral firearm injury with pistols. The canine supine position was fixed on the operating table immediately after modeling, followed by tracheal intubation, ECG monitoring, cardiopulmonary resuscitation and other rescue measures to monitor the vital signs such as heart rate and blood pressure after injury. No other treatment except cardiac extrusion and artificial respiration sac extrusion. Abdominal surgery was performed immediately under aseptic conditions, ileocecal part was searched by laparotomy, and ileal tissue at the end of 1-3cm was immediately centrifuged and preserved, then the stump was anastomosed. According to the time gradient: 0 hours, 0.5 hours, 1 hour, 1.5 hours, 2 hours, 5 samples of peripheral blood were collected from each group of dogs, and then centrifuged immediately. In the process of delaying the life of experimental dogs as far as possible, intravenous fluid supply. Enzyme linked immunosorbent assay (Enzyme-LinkedImmunosorbent Assay,ELISA) was used to quantitatively detect the concentration of GnRH and SS in peripheral blood, and Westernblot was used to detect the expression of GnRH receptor and SS receptor at different time points in the terminal ileum tissue. Results: 1. The change of plasma GnRH concentration in each group: at 0 h, there was no significant difference in plasma GnRH concentration in 8 experimental groups. At 0.5 h, the concentration of GnRH in plasma of experimental dogs decreased obviously, which was related to their self-regulation, and the concentration of GnRH increased gradually with the prolongation of time after stress, and obviously exceeded the initial concentration (0 h). 2. The changes of plasma SS concentration in each group of experimental dogs: there was no significant difference in plasma SS concentration in each of the eight experimental groups at 0 h, and the SS concentration in plasma of each group decreased significantly at 0.5 h, which was related to its self-regulation. The concentration of SS increased with the prolongation of stress time and exceeded the initial concentration (0 h). 3. The expression of GnRH receptor and SS receptor in the terminal ileum of group A and B: the expression of GnRH receptor and SS receptor in terminal ileum were analyzed by Westernblot, and the expression of GnRHR,SSTR increased under acute stress. There was a positive correlation between GnRH receptor, SS receptor and corresponding GnRH concentration and SS concentration. Conclusion: 1. The concentration of GnRH and the expression of its receptor increased in the plasma of acute terminal colitis after craniocerebral firearm injury, 2. The concentration of SS and the expression of receptor in plasma of acute terminal gynecitis with craniocerebral firearm injury increased; 3. GnRH,SS and its receptors are involved in the regulation of stress on acute terminal colitis, and there is a positive correlation of intensity in a certain period of time.
【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R574
【參考文獻(xiàn)】
相關(guān)期刊論文 前10條
1 漆建,余定庸,唐文國(guó);重型顱腦損傷后高血糖與預(yù)后的關(guān)系[J];中華神經(jīng)醫(yī)學(xué)雜志;2003年01期
2 趙東升,裴國(guó)獻(xiàn),魏寬海,羅炳德,王前;高溫高濕環(huán)境肢體火器傷病理形態(tài)學(xué)變化[J];第一軍醫(yī)大學(xué)學(xué)報(bào);2001年02期
3 余俐,徐如祥,彭蘋(píng),謝培增,朱紅勝,王松青;7.62mm手槍彈顱腦火器傷動(dòng)物模型的建立[J];第一軍醫(yī)大學(xué)學(xué)報(bào);2004年12期
4 曹寶花;顱腦損傷后胃腸道功能的改變[J];國(guó)外醫(yī)學(xué).護(hù)理學(xué)分冊(cè);2000年03期
5 劉壽;格日力;;應(yīng)激性胃黏膜病變相關(guān)因子的研究進(jìn)展[J];國(guó)際消化病雜志;2010年01期
6 周國(guó)華;秦穩(wěn);胡詠;;TLR2、TLR4在實(shí)驗(yàn)性末端回腸炎發(fā)病過(guò)程中的變化及意義[J];華南國(guó)防醫(yī)學(xué)雜志;2011年05期
7 曹碧波;談超;丁明亮;安飛;楊珉;;生長(zhǎng)抑素和胃泌素檢測(cè)在合并上消化道出血顱腦損傷中的意義[J];中國(guó)實(shí)用神經(jīng)疾病雜志;2012年09期
8 黃祝;蔣超;徐會(huì)茹;王晨陽(yáng);侯林;姚兵;;恐懼應(yīng)激對(duì)大鼠小腸促性腺激素釋放激素受體的影響[J];醫(yī)學(xué)研究生學(xué)報(bào);2007年10期
9 時(shí)姍姍;陶曉倩;韓雪峰;盧坤剛;張艷梅;柳海燕;姚兵;;光照應(yīng)激對(duì)SD大鼠小腸促性腺激素釋放激素受體表達(dá)的影響[J];醫(yī)學(xué)研究生學(xué)報(bào);2009年11期
10 于輝天;葉華衛(wèi);江先福;麥榮康;宋_g;史小軍;;高血壓腦出血后腦脊液促性腺激素釋放激素(GnRH)臨床分析[J];海南醫(yī)學(xué)院學(xué)報(bào);2013年07期
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