【摘要】：目的探討中腦星形膠質(zhì)細(xì)胞源性神經(jīng)營(yíng)養(yǎng)因子(MANF)蛋白在慢性乙型肝炎病毒(HBV)感染患者肝纖維化發(fā)生發(fā)展中的作用及其與臨床特點(diǎn)的相關(guān)性。方法采用相對(duì)和絕對(duì)定量同位素標(biāo)記(ITRAQ)蛋白質(zhì)組學(xué)和免疫組化法檢測(cè)肝臟穿刺組織中MANF蛋白的表達(dá),分析其表達(dá)水平與肝臟炎癥纖維化分期的關(guān)系;采用實(shí)時(shí)熒光定量PCR技術(shù)檢測(cè)正常對(duì)照(NC)、乙肝病毒攜帶者(ASC)、慢性乙型肝炎患者(CHB)及乙肝后肝硬化患者(LC)外周血白細(xì)胞中MANF mRNA的表達(dá)水平,分析其與不同階段慢性HBV感染者的臨床病毒學(xué)和生化學(xué)指標(biāo)的相關(guān)性。結(jié)果 MANF蛋白主要在肝細(xì)胞質(zhì)中表達(dá),其表達(dá)水平隨著肝臟炎癥及纖維化的程度加重而升高。NC組、ASC組和CHB組分別與LC組比較,MANF mRNA的表達(dá)差異均有統(tǒng)計(jì)學(xué)意義(P0.01)。MANF mRNA的表達(dá)水平在乙肝病毒表面抗原(HBsA g)1.5×106IU/L、(1.5×106~2.0×107)IU/L和2.0×107IU/L 3組間差異有統(tǒng)計(jì)學(xué)意義(P0.05);在乙肝病毒e抗原(HBeA g)陽(yáng)性組與陰性組之間差異有統(tǒng)計(jì)學(xué)意義(P0.01);在膽紅素正常組與異常組之間差異有統(tǒng)計(jì)學(xué)意義(P0.01)。結(jié)論 MANF蛋白可能參與了慢性HBV感染者肝纖維化的發(fā)生發(fā)展,并與其臨床特點(diǎn)相關(guān)。
[Abstract]:Objective to investigate the role of mesencephalic astrocyte-derived neurotrophic factor (MANF) protein in the development of hepatic fibrosis in patients with chronic hepatitis B virus (HBV) infection and its correlation with clinical features. Methods relative and absolute quantitative isotope labeling (ITRAQ) proteomics and immunohistochemical method were used to detect the expression of MANF protein in liver puncture tissues, and the relationship between the expression level and the stage of hepatic inflammatory fibrosis was analyzed. The expression of MANF mRNA in peripheral blood leukocytes of patients with (ASC), chronic hepatitis B and posthepatitic cirrhosis of (LC) was detected by real-time fluorescence quantitative PCR. To analyze the correlation between the clinical virology and biochemistry of chronic HBV infection in different stages. Results MANF protein was mainly expressed in the cytoplasm of liver, and its expression level increased with the exacerbation of liver inflammation and fibrosis. NC group, ASC group and CHB group were compared with LC group, respectively. There were statistically significant differences in the expression of MANF mRNA (P0.01). MANF mRNA expression level in (HBsA g) 1.5 脳 106 IUP / L, P 0.01). There was significant difference between (1.5 脳 106) IU/L and 2.0 脳 107IU/L groups (P0.05). There was significant difference between positive and negative groups of hepatitis B virus e antigen (HBeA g) (P0.01), and between normal group of bilirubin and abnormal group (P0.01). Conclusion MANF protein may be involved in the occurrence and development of hepatic fibrosis in patients with chronic HBV infection and may be related to its clinical features.
【作者單位】： 安徽醫(yī)科大學(xué)第二附屬醫(yī)院肝病科;安徽醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院;
【基金】：安徽高校省級(jí)自然科學(xué)研究項(xiàng)目(編號(hào):KJ2014A107)
【分類號(hào)】：R575.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 賈繼東;李蘭娟;;慢性乙型肝炎防治指南(2010年版)[J];臨床肝膽病雜志;2011年01期

2 陳瀅;李成錦;楊文;程里;安然;都建;沈玉君;馮利杰;沈玉先;;MANF對(duì)內(nèi)質(zhì)網(wǎng)應(yīng)激誘導(dǎo)的細(xì)胞凋亡的保護(hù)作用[J];安徽醫(yī)科大學(xué)學(xué)報(bào);2013年11期

3 陸鵬;姜同翠;陳露;沈玉君;沈玉先;方圣云;;乙肝病毒HBx基因穩(wěn)轉(zhuǎn)細(xì)胞系的建立及其誘導(dǎo)的內(nèi)質(zhì)網(wǎng)應(yīng)激[J];安徽醫(yī)科大學(xué)學(xué)報(bào);2014年03期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 高銀杰;張敏;蘇海濱;劉振文;李捍衛(wèi);;乙型肝炎相關(guān)性肝移植后乙型肝炎復(fù)發(fā)的臨床特點(diǎn)分析[J];國(guó)際消化病雜志;2012年04期

2 吳成久;;替比夫定治療失代償期乙肝肝硬化的療效觀察[J];承德醫(yī)學(xué)院學(xué)報(bào);2013年02期

3 何小珊;朱輝超;朱文標(biāo);;慢性乙型肝炎產(chǎn)婦臍帶血HBV-DNA檢測(cè)的臨床意義[J];中國(guó)醫(yī)藥科學(xué);2013年18期

4 許大成;古麗米娜·玉山;張朝霞;嚴(yán)雪梅;蔣靜;;HBV慢性感染者血清中MBL、MASP和C3b水平檢測(cè)的臨床意義[J];國(guó)際檢驗(yàn)醫(yī)學(xué)雜志;2013年24期

5 楊麗莎;梁志清;范劍薇;吳淋玲;蘇元英;張兵;;慢性乙型肝炎病毒感染不同免疫狀態(tài)主要證候的研究[J];中華中醫(yī)藥雜志;2014年01期

6 陳奕伸;洪仲思;梁嘉碧;;益肝靈軟膠囊聯(lián)合恩替卡韋對(duì)慢性乙肝患者肝功能改善情況觀察[J];北方藥學(xué);2014年07期

7 李洪;;干擾素聯(lián)合恩替卡韋對(duì)慢性乙肝患者血清纖維化指標(biāo)的影響[J];北方藥學(xué);2014年09期

8 劉紅霞;程敏;黎希予;;恩替卡韋聯(lián)合丹菊三苓湯治療慢性乙型肝炎療效觀察[J];廣西中醫(yī)藥;2014年04期

9 郜玉峰;湯磊;潘高峰;殷宏娟;葉s，

本文編號(hào)：2314206