【摘要】：目的探討趨化因子2(CCL2)對(duì)肝再生的影響以及作用機(jī)制。方法大鼠隨機(jī)分為3組,每組10只。液壓轉(zhuǎn)基因技術(shù)將質(zhì)粒轉(zhuǎn)入大鼠體內(nèi),6 h后熒光顯微鏡下觀察轉(zhuǎn)染效率。稱量再生肝重量,計(jì)算肝再生率和肝臟指數(shù)以觀察肝臟再生情況。測(cè)量血清中谷丙轉(zhuǎn)氨酶(ALT)、谷草轉(zhuǎn)氨酶(AST)與總膽紅素(TBIL)的含量以評(píng)估肝臟功能情況。蘇丹Ⅳ染色觀測(cè)脂肪聚積情況。Real-time PCR檢測(cè)脂肪代謝相關(guān)基因的表達(dá)。Western blotting檢測(cè)磷酸化絲裂原細(xì)胞外激酶1/2(p-MEK1/2)和磷酸化細(xì)胞外信號(hào)調(diào)節(jié)激酶l/2(p-ERKl/2)的表達(dá)情況。結(jié)果轉(zhuǎn)質(zhì)粒后6 h各組綠色熒光蛋白表達(dá)量均大于30%。p EGFP-N1-CCL2轉(zhuǎn)染組肝再生率、肝臟指數(shù)、ALT、AST和TBIL含量均高于p EGFP-N1組。隨轉(zhuǎn)基因時(shí)間延長(zhǎng),脂肪代謝相關(guān)基因表達(dá)量增加,有較多猩紅色脂肪滴出現(xiàn),pMEK1/2和p-ERKl/2表達(dá)量增多。結(jié)論趨化因子2可能通過MEK/ERK通路增加脂肪合成,促進(jìn)肝臟再生。
[Abstract]:Objective to investigate the effect and mechanism of chemokine 2 (CCL2) on liver regeneration. Methods the rats were randomly divided into 3 groups with 10 rats in each group. The transfection efficiency was observed under fluorescence microscope for 6 hours. Weight of regenerated liver, liver regeneration rate and liver index were calculated to observe liver regeneration. Serum alanine aminotransferase (ALT), glutamic oxalacetic transaminase (AST) and total bilirubin (TBIL) were measured to evaluate liver function. Real-time PCR was used to detect the expression of genes related to fat metabolism. The expression of extracellular phosphomitogen kinase 1 / 2 (p-MEK1/2) and phosphorylated signal regulated kinase l / 2 (p-ERKl/2) were detected by Real-time PCR. Results the expression of green fluorescent protein in each group was higher than that in 30 路p EGFP-N1-CCL2 group at 6 h after transfection, and the liver regeneration rate, liver index, ALT,AST and TBIL content were higher than those in p EGFP-N1 group. With the extension of transgenic time, the expression of fat metabolism-related genes increased, more scarlet fat droplets appeared, and the expression of pMEK1/2 and p-ERKl/2 increased. Conclusion chemokine 2 may increase fat synthesis and promote liver regeneration through MEK/ERK pathway.
【作者單位】： 新鄉(xiāng)醫(yī)學(xué)院生命科學(xué)技術(shù)學(xué)院;新鄉(xiāng)醫(yī)學(xué)院法醫(yī)學(xué)系;
【基金】：河南省高等學(xué)校重點(diǎn)科研項(xiàng)目計(jì)劃(15A180020)
【分類號(hào)】：R575

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