【摘要】：目的探討趨化因子2(CCL2)對肝再生的影響以及作用機制。方法大鼠隨機分為3組,每組10只。液壓轉基因技術將質粒轉入大鼠體內,6 h后熒光顯微鏡下觀察轉染效率。稱量再生肝重量,計算肝再生率和肝臟指數(shù)以觀察肝臟再生情況。測量血清中谷丙轉氨酶(ALT)、谷草轉氨酶(AST)與總膽紅素(TBIL)的含量以評估肝臟功能情況。蘇丹Ⅳ染色觀測脂肪聚積情況。Real-time PCR檢測脂肪代謝相關基因的表達。Western blotting檢測磷酸化絲裂原細胞外激酶1/2(p-MEK1/2)和磷酸化細胞外信號調節(jié)激酶l/2(p-ERKl/2)的表達情況。結果轉質粒后6 h各組綠色熒光蛋白表達量均大于30%。p EGFP-N1-CCL2轉染組肝再生率、肝臟指數(shù)、ALT、AST和TBIL含量均高于p EGFP-N1組。隨轉基因時間延長,脂肪代謝相關基因表達量增加,有較多猩紅色脂肪滴出現(xiàn),pMEK1/2和p-ERKl/2表達量增多。結論趨化因子2可能通過MEK/ERK通路增加脂肪合成,促進肝臟再生。
[Abstract]:Objective to investigate the effect and mechanism of chemokine 2 (CCL2) on liver regeneration. Methods the rats were randomly divided into 3 groups with 10 rats in each group. The transfection efficiency was observed under fluorescence microscope for 6 hours. Weight of regenerated liver, liver regeneration rate and liver index were calculated to observe liver regeneration. Serum alanine aminotransferase (ALT), glutamic oxalacetic transaminase (AST) and total bilirubin (TBIL) were measured to evaluate liver function. Real-time PCR was used to detect the expression of genes related to fat metabolism. The expression of extracellular phosphomitogen kinase 1 / 2 (p-MEK1/2) and phosphorylated signal regulated kinase l / 2 (p-ERKl/2) were detected by Real-time PCR. Results the expression of green fluorescent protein in each group was higher than that in 30 路p EGFP-N1-CCL2 group at 6 h after transfection, and the liver regeneration rate, liver index, ALT,AST and TBIL content were higher than those in p EGFP-N1 group. With the extension of transgenic time, the expression of fat metabolism-related genes increased, more scarlet fat droplets appeared, and the expression of pMEK1/2 and p-ERKl/2 increased. Conclusion chemokine 2 may increase fat synthesis and promote liver regeneration through MEK/ERK pathway.
【作者單位】： 新鄉(xiāng)醫(yī)學院生命科學技術學院;新鄉(xiāng)醫(yī)學院法醫(yī)學系;
【基金】：河南省高等學校重點科研項目計劃(15A180020)
【分類號】：R575

【相似文獻】

相關期刊論文 前10條

1 韓淑媛;黃志強;劉永雄;于國;咎世明;張曙光;李暉;;大鼠肝部分切除后肝再生過程的動態(tài)觀察[J];傳染病信息;1995年02期

2 唐天勛,樊克武,井清源;大鼠肝、胰十二指腸聯(lián)合切除對殘肝再生的影響[J];南京醫(yī)科大學學報;2002年01期

3 黃志剛,劉殿武;肝再生增強因子的研究進展[J];中國病理生理雜志;2002年05期

4 王閣;肝再生信號轉錄調控進展[J];國外醫(yī)學(消化系疾病分冊);2003年04期

5 趙利峰,徐存拴,王林嵩,章靜波;調控肝再生的基因和生長因子的研究進展[J];解剖學報;2004年03期

6 許翠萍,韓德五,張楊,趙元昌,尹鐳;肝部分切除大鼠腸源性內毒素血癥與肝再生的關系[J];世界華人消化雜志;2004年12期

7 李瀚e，

本文編號：2286103