【摘要】：目的:純化的HCV多中和抗原表位及HCV包膜蛋白E2嵌合的HBV S抗原病毒樣顆粒免疫新西蘭兔,測定免疫血清內的中和抗體。分析中和抗體對HCVpp感染Huh7.5的中和作用。方法:純化的HCV多中和抗原表位及包膜蛋白E2嵌合的HBV S抗原病毒樣顆粒(VLPs-MEp S、VLPs-E2S)10μg皮下接種新西蘭兔,間隔2周共免疫3次,采集不同時間免疫兔血清,ELISA方法測定血清內的抗體,PBS組作為對照;制備1b型HCVpp,觀察血清抗體對HCVpp感染Huh7.5的中和作用,對免疫血清保護性進行初步評價。結果:免疫后血清中產(chǎn)生一定水平的中和抗體,血清中和抗體測定VLPs-MEp S明顯高于VLPs-E2S(P0.05)。VLPs-MEp S與VLPs-E2S組均顯著高于對照PBS組(P0.01)。對HCVpp抑制作用,VLPs-MEp S高于VLPs-E2S組(P0.05),最高中和率可達61.49%,二者均高于對照組(P0.01)。結論:嵌合病毒樣顆粒免疫新西蘭兔后產(chǎn)生一定水平中和抗體,該中和抗體具有保護作用,為研發(fā)中和抗體表位疫苗奠定基礎。
[Abstract]:Aim: to immunize New Zealand rabbits with purified HCV polyneutralizing antigen epitopes and HCV envelope protein E2 chimeric HBV S antigen-like particles and detect neutralizing antibodies in immune serum. Neutralizing effect of neutralizing antibody on HCVpp infected Huh7.5. Methods: purified HCV polyneutralizing antigen epitopes and envelope protein E2 chimeric HBV S antigen-like particles (VLPs-MEp SVLPs-E2S) were inoculated subcutaneously into New Zealand rabbits. The rabbits were immunized three times at intervals of 2 weeks. The serum of rabbits was immunized at different times, the antibody in the serum was determined by ELISA method, the PBS group was used as control group, and the neutralization effect of serum antibody on HCVpp infected Huh7.5 was observed by preparing 1b HCVpp, and the protective effect of the immune serum was evaluated preliminarily. Results: after immunization, a certain level of neutralizing antibody was produced in serum, and VLPs-MEp S in serum neutralization antibody was significantly higher than that in VLPs-E2S (P0.05). VLPs-MEp S and VLPs-E2S group were significantly higher than control PBS group (P0.01). The inhibitory effect of VLPs-MEp S on HCVpp was higher than that of VLPs-E2S group (P0.05), and the highest neutralization rate was 61.49%, both of which were higher than that of control group (P0.01). Conclusion: chimeric virus-like particles produced a certain level of neutralizing antibody after immunizing New Zealand rabbits. The neutralizing antibody has protective effect and lays a foundation for the development of neutralizing and anti-epitope vaccine.
【作者單位】： 第四軍醫(yī)大學唐都醫(yī)院臨床實驗與檢驗科;第四軍醫(yī)大學實驗動物中心;第四軍醫(yī)大學基礎部微生物學教研室;
【基金】：國家自然科學基金項目(No.31370924,31570933)
【分類號】：R512.63
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本文編號：2280470