亞硒酸鈉通過(guò)逆轉(zhuǎn)甲狀腺激素轉(zhuǎn)化異常改善非酒精性脂肪肝病變
發(fā)布時(shí)間：2018-07-29 06:15
【摘要】：目的 探討在NAFLD發(fā)生、發(fā)展過(guò)程中甲狀腺激素的轉(zhuǎn)化情況，了解甲狀腺激素水平與脂代謝紊亂的關(guān)系。研究應(yīng)用亞硒酸鈉干預(yù)甲狀腺激素轉(zhuǎn)化異常能否防治NAFLD。 方法 （1）健康雄性SD大鼠64只，隨機(jī)分為模型組和正常組，每組32只。模型組采用高脂飼料喂養(yǎng)建立非酒精性脂肪肝模型，正常組則采用常規(guī)飼料喂養(yǎng)。分別于0、4、8、12周時(shí)觀察各組大鼠體重、肝濕重、肝指數(shù)，檢測(cè)上述各時(shí)間點(diǎn)各組大鼠血清AST、ALT、TC、TG、HDL-C、LDL-C、T3、T4、rT3和肝臟硒含量與5′-脫碘酶活性。同時(shí)取各組各時(shí)間點(diǎn)病理切片行HE和Masson染色，觀察大鼠肝組織病理學(xué)改變。 （2）健康雄性SD大鼠96只，隨機(jī)分為正常組、模型組和亞硒酸鈉組，每組32只，正常組和模型組喂養(yǎng)方法如上述，亞硒酸鈉組在高脂飲食喂養(yǎng)4周后再輔以亞硒酸鈉（0.2mg/kg）灌胃。各組大鼠一般指標(biāo)、血清學(xué)指標(biāo)及病理學(xué)檢測(cè)如上所述。 結(jié)果 1、（1）一般指標(biāo)：模型組大鼠12周時(shí)體重、肝濕重及肝指數(shù)顯著高于對(duì)照組（P＜0.05）。（2）病理學(xué)檢測(cè)：模型組大鼠12周時(shí)肝組織可見(jiàn)明顯脂肪變，炎性細(xì)胞浸潤(rùn)，匯管區(qū)纖維化。（3）血清學(xué)指標(biāo)：模型組大鼠12周時(shí)血清AST、ALT、TC、TG、LDL-C水平均有顯著的升高，與之前各時(shí)間點(diǎn)相比，差異均具有統(tǒng)計(jì)學(xué)意義（P＜0.05）。（4）甲狀腺激素水平：模型組血清T3和T4水平逐漸下降，rT3水平升高，在8周和12周時(shí)，與正常組相比，差異均具有統(tǒng)計(jì)學(xué)意義（P＜0.05）。（5）肝臟5′-脫碘酶活性及硒含量：模型組大鼠5′-脫碘酶活性和硒含量水平逐漸下降，與正常組相比，，差異均具有統(tǒng)計(jì)學(xué)意義（P＜0.05）。 2、（1）一般指標(biāo)：亞硒酸鈉組大鼠12周時(shí)體重、肝濕重及肝指數(shù)均小于模型組（P＜0.05）。（2）病理學(xué)檢測(cè)：亞硒酸鈉組大鼠12周時(shí)肝組織脂肪沉積及纖維化程度顯著低于模型組。（3）血清學(xué)指標(biāo)：亞硒酸鈉組大鼠的血清AST、ALT、TC、TG、LDL-C水平4周后低于同時(shí)間點(diǎn)的模型組，差異具有統(tǒng)計(jì)學(xué)意義（P＜0.05）。（4）甲狀腺激素水平：亞硒酸鈉組大鼠血清T3、T4水平有所下降，rT3水平稍有升高，12周時(shí)與正常組接近，與模型組相比，差異具有統(tǒng)計(jì)學(xué)意義（P＜0.05）。（5）肝臟5′-脫碘酶活性及硒含量：亞硒酸鈉組大鼠肝臟5′-脫碘酶活性和硒含量在4周后逐漸升高，12周時(shí)水平與正常組接近，與模型組相比，差異具有統(tǒng)計(jì)學(xué)意義（P＜0.05）。 結(jié)論 1.通過(guò)高脂誘導(dǎo)的NAFLD模型大鼠血清T3、T4水平顯著下降，rT3水平則顯著上升，提示甲狀腺激素水平異常與NAFLD的發(fā)病密切相關(guān)。 2. NAFLD大鼠肝臟5′-脫碘酶活性及硒含量顯著下降，與甲狀腺激素轉(zhuǎn)化異常相關(guān)。 3.亞硒酸鈉可使NAFLD大鼠肝組織硒含量及5′-脫碘酶活性升高，甲狀腺激素轉(zhuǎn)化異常狀態(tài)得到改善，血清血脂紊亂和肝臟脂質(zhì)沉積及纖維化程度減輕。
[Abstract]:Objective to investigate the relationship between thyroid hormone level and lipid metabolism disorder during the development of NAFLD. To study the effect of sodium selenite on thyroid hormone transformation. Methods (1) 64 healthy male SD rats were randomly divided into model group and normal group with 32 rats in each group. The model group was fed with high fat diet to establish the model of non alcoholic fatty liver, while the normal group was fed with conventional diet. The body weight, liver wet weight and liver index were observed at the 12th week of each group, and the serum levels of ASTT TCU, HDL-C, LDL-C, LDL-C, T4 rT3 and the activity of 5-tida-deiodinase in the liver were measured at 12 weeks. At the same time, the pathological sections of each group were stained with HE and Masson. (2) 96 healthy male Sprague-Dawley rats were randomly divided into normal group, model group and sodium selenite group with 32 rats in each group. Sodium selenite group was fed with sodium selenite (0.2mg/kg) for 4 weeks after high fat diet. The general index, serological index and pathological examination of rats in each group were as mentioned above. Results 1, (1) General measures: the body weight, liver wet weight and liver index in the model group were significantly higher than those in the control group at 12 weeks (P < 0. 05). (2). (3) serological indicators: the serum levels of LDL-C in serum of rats in the model group were significantly increased at 12 weeks, compared with the previous time points. The serum T3 and T4 levels of the model group decreased gradually and the RT3 level increased gradually, and at the 8th and 12th week, compared with the normal group, the serum T3 and T4 levels in the model group were significantly higher than those in the normal group, and the difference was statistically significant (P < 0. 05). (4). The difference was statistically significant (P < 0. 05). (5). The activity and selenium content of 5- deiodinase and selenium in the liver of the model group decreased gradually, compared with the normal group. The difference was statistically significant (P < 0.05). 2, (1) General measures: the body weight of sodium selenite group was 12 weeks, Liver wet weight and liver index were lower than model group (P < 0. 05). (2) pathological examination: the degree of hepatic fat deposition and liver fibrosis in sodium selenite group was significantly lower than that in model group at 12 weeks. (3) serological index: serum ASTTA TCU TGG LDL-C in sodium selenite group was significantly lower than that in model group (P < 0. 05). (2). After 4 weeks, the level was lower than that of the model group at the same time point. The difference was statistically significant (P < 0. 05). (4). The serum T3T4 level of sodium selenite group decreased slightly and the RT3 level increased slightly after 12 weeks, which was similar to that of the normal group, and compared with the model group. The difference was statistically significant (P < 0. 05). (5). The activity and selenium content of liver 5- deiodinase and selenium in sodium selenite group were increased gradually after 4 weeks and were close to those in the normal group at 12 weeks, compared with the model group, and the content of selenium in the liver of the sodium selenite group was similar to that of the control group. The difference was statistically significant (P < 0.05). Conclusion 1. The serum T3T4 level in NAFLD model rats induced by hyperlipidemia was significantly decreased and RT3 level was significantly increased, suggesting that the abnormal thyroid hormone level was closely related to the pathogenesis of NAFLD. 2. The activity of 5- deiodinase and the content of selenium in the liver of NAFLD rats were significantly decreased, which was related to the abnormal transformation of thyroid hormone. Sodium selenite could increase the content of selenium in liver tissue and the activity of 5- deiodinase in NAFLD rats, improve the abnormal state of thyroid hormone transformation, reduce the disorder of serum lipids and liver lipid deposition and reduce the degree of hepatic fibrosis.
【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R575.5
本文編號(hào)：2151790
[Abstract]:Objective to investigate the relationship between thyroid hormone level and lipid metabolism disorder during the development of NAFLD. To study the effect of sodium selenite on thyroid hormone transformation. Methods (1) 64 healthy male SD rats were randomly divided into model group and normal group with 32 rats in each group. The model group was fed with high fat diet to establish the model of non alcoholic fatty liver, while the normal group was fed with conventional diet. The body weight, liver wet weight and liver index were observed at the 12th week of each group, and the serum levels of ASTT TCU, HDL-C, LDL-C, LDL-C, T4 rT3 and the activity of 5-tida-deiodinase in the liver were measured at 12 weeks. At the same time, the pathological sections of each group were stained with HE and Masson. (2) 96 healthy male Sprague-Dawley rats were randomly divided into normal group, model group and sodium selenite group with 32 rats in each group. Sodium selenite group was fed with sodium selenite (0.2mg/kg) for 4 weeks after high fat diet. The general index, serological index and pathological examination of rats in each group were as mentioned above. Results 1, (1) General measures: the body weight, liver wet weight and liver index in the model group were significantly higher than those in the control group at 12 weeks (P < 0. 05). (2). (3) serological indicators: the serum levels of LDL-C in serum of rats in the model group were significantly increased at 12 weeks, compared with the previous time points. The serum T3 and T4 levels of the model group decreased gradually and the RT3 level increased gradually, and at the 8th and 12th week, compared with the normal group, the serum T3 and T4 levels in the model group were significantly higher than those in the normal group, and the difference was statistically significant (P < 0. 05). (4). The difference was statistically significant (P < 0. 05). (5). The activity and selenium content of 5- deiodinase and selenium in the liver of the model group decreased gradually, compared with the normal group. The difference was statistically significant (P < 0.05). 2, (1) General measures: the body weight of sodium selenite group was 12 weeks, Liver wet weight and liver index were lower than model group (P < 0. 05). (2) pathological examination: the degree of hepatic fat deposition and liver fibrosis in sodium selenite group was significantly lower than that in model group at 12 weeks. (3) serological index: serum ASTTA TCU TGG LDL-C in sodium selenite group was significantly lower than that in model group (P < 0. 05). (2). After 4 weeks, the level was lower than that of the model group at the same time point. The difference was statistically significant (P < 0. 05). (4). The serum T3T4 level of sodium selenite group decreased slightly and the RT3 level increased slightly after 12 weeks, which was similar to that of the normal group, and compared with the model group. The difference was statistically significant (P < 0. 05). (5). The activity and selenium content of liver 5- deiodinase and selenium in sodium selenite group were increased gradually after 4 weeks and were close to those in the normal group at 12 weeks, compared with the model group, and the content of selenium in the liver of the sodium selenite group was similar to that of the control group. The difference was statistically significant (P < 0.05). Conclusion 1. The serum T3T4 level in NAFLD model rats induced by hyperlipidemia was significantly decreased and RT3 level was significantly increased, suggesting that the abnormal thyroid hormone level was closely related to the pathogenesis of NAFLD. 2. The activity of 5- deiodinase and the content of selenium in the liver of NAFLD rats were significantly decreased, which was related to the abnormal transformation of thyroid hormone. Sodium selenite could increase the content of selenium in liver tissue and the activity of 5- deiodinase in NAFLD rats, improve the abnormal state of thyroid hormone transformation, reduce the disorder of serum lipids and liver lipid deposition and reduce the degree of hepatic fibrosis.
【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R575.5
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相關(guān)期刊論文 前4條
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本文編號(hào)：2151790
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