本文選題：樂復能 + 美沙拉嗪��； 參考：《中南大學》2014年碩士論文

【摘要】：目的 研究觀察樂復能(novaferon)對葡聚糖硫酸鈉(DSS)誘導的實驗性結(jié)腸炎小鼠模型腸粘膜內(nèi)趨化因子IL-8和MCP-1表達的影響。 方法 將70只Balb/C雌性小鼠,SPF級,6-8周齡,18-22g,分成7組,每組10只。分別為正常組,模型組,樂復能高、中、低劑量組、美沙拉嗪組及潑尼松組。予以4%DSS液自由飲用7天造實驗性結(jié)腸炎小鼠模型。造模第二天開始分別給予正常組和模型組無菌生理鹽水灌胃,美沙拉嗪組、潑尼松組分別給予美沙拉嗪和潑尼松灌胃,樂復能各劑量組分別給予0.1ug/ml、0.2ug/ml、0.3ug/ml樂復能腹腔注射,0.2m1/次,以上處理均為每日一次。造模第八天處死所有小鼠。實驗過程中觀察小鼠的體重、活動情況、大便隱血情況,對小鼠進行DAI評分,處死小鼠取得標本后觀察組織學損傷評分,免疫組化檢測小鼠腸粘膜內(nèi)IL-8、MCP-1的表達。 結(jié)果 1、除正常組小鼠外,其他用DSS干預的小鼠均不同程度的出現(xiàn)便血、體重下降、活動度減退的情況,小鼠的DAI積分逐漸增加,經(jīng)樂復能、美沙拉嗪、潑尼松處理后的小鼠的一般情況、DAI積分、組織學損傷評分有所下降。 2、和模型組相比較,樂復能高劑量組、美沙拉嗪組、潑尼松組實驗性結(jié)腸炎小鼠模型腸粘膜內(nèi)IL-8、MCP-1的表達下降(P0.05),有統(tǒng)計學意義。 結(jié)論 葡聚糖硫酸鈉(DSS)誘導的實驗性結(jié)腸炎小鼠模型和潰瘍性結(jié)腸炎(UC)的臨床癥狀及組織學特點類似；樂復能、美沙拉嗪、潑尼松可以下調(diào)實驗性結(jié)腸炎小鼠模型腸粘膜內(nèi)IL-8、MCP-1的表達。
[Abstract]:Objective to investigate the effect of Lefongneng Novaferon on the expression of IL-8 and MCP-1 in intestinal mucosa of mice with experimental colitis induced by dextran sodium sulfate (DSS). Methods 70 Balb / C female mice were divided into 7 groups with 10 rats in each group. They were normal group, model group, high, middle and low dose group, mesalazine group and prednisone group. The mice model of experimental colitis was established with 4 DSS solution for 7 days. On the second day of model making, the normal group and the model group were given aseptic saline, the mesalazine group and prednisone group were given intragastric administration of mesalazine and prednisone respectively, and each dose group was given 0.1ugml / ml 0.2ugP / ml Loflon intraperitoneal injection of 0.2m1g / ml, respectively. The above treatment is once a day. On the eighth day, all the mice were killed. The weight, activity and fecal occult blood of the mice were observed during the experiment. Dai score was used to evaluate the mice. The histological injury score was observed after the mice were killed. The expression of IL-8 and MCP-1 in the intestinal mucosa of the mice was detected by immunohistochemistry. Results 1 except in the normal group, the other mice treated with DSS had hematochezia, decreased body weight and decreased activity, the Dai score of the mice increased gradually, and the rats were treated with Leferin and mesalazine. After prednisone treatment, Dai score and histological injury score were decreased in mice treated with prednisone. 2Compared with the model group, the high dose group of loflon, the group of mesalazine, In prednisone group, the expression of IL-8 and MCP-1 in intestinal mucosa of experimental colitis mice was decreased (P 0.05). Conclusion the clinical symptoms and histological characteristics of experimental colitis mice induced by dextran sodium sulfate (DSS) and ulcerative colitis were similar. Prednisone could down-regulate the expression of IL-8 and MCP-1 in intestinal mucosa of experimental colitis mice.
【學位授予單位】：中南大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R574.62

【參考文獻】

相關(guān)期刊論文 前1條

1 Talia Zenlea;Mark A Peppercorn;;Immunosuppressive therapies for inflammatory bowel disease[J];World Journal of Gastroenterology;2014年12期

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本文編號：2019170