本文選題：酒精性肝損傷 + Hoechst染色　； 參考：《中國臨床藥理學(xué)雜志》2017年21期

【摘要】：目的研究小鼠酒精性肝損傷過程中肝細(xì)胞凋亡的變化規(guī)律。方法按照體重將健康KM雄性小鼠隨機(jī)分為2組:對照組(n=10),模型組(n=80)。模型組給予56°白酒(7.5μL·g~(-1)·d~(-1))灌胃,持續(xù)8周。分別于1,2,3,4,5,6,7,8周脫臼處死小鼠,取肝組織;對照組小鼠等量蒸餾水灌胃后處死。進(jìn)行蘇木素-伊紅染色(HE)和Hoechst_33258染色,檢測小鼠酒精性肝損傷中肝細(xì)胞的凋亡變化;熒光顯微鏡下辨認(rèn)和計數(shù)正常肝細(xì)胞和凋亡肝細(xì)胞,計算細(xì)胞凋亡率。結(jié)果第3周,模型組小鼠發(fā)生急性肝損傷改變,表現(xiàn)為炎癥細(xì)胞浸潤加劇;到第8周,進(jìn)展為慢性肝損傷,表現(xiàn)為肝組織水樣變,脂肪空泡。第1,2,3周,模型組的凋亡率分別為(9.28±0.41)%,(13.20±0.81)%,(20.43±0.97)%,與對照組的(1.38±0.18)%比較,差異均有統(tǒng)計學(xué)意義(均P0.05)。第8周末,凋亡率達(dá)到(51.99±1.70)%,與對照組相比,差異有統(tǒng)計學(xué)意義(P0.01)。結(jié)論小鼠酒精性肝損傷程度與肝細(xì)胞凋亡的發(fā)生發(fā)展密切相關(guān)。
[Abstract]:Objective to study the changes of hepatocyte apoptosis during alcoholic liver injury in mice. Methods healthy km male mice were randomly divided into two groups according to their body weight: control group (n = 10) and model group (n = 80). The model group was treated with 56 擄liquor (7.5 渭 L / g) D ~ (- 1) ~ (-1) by gastric perfusion for 8 weeks. The mice were killed after 8 weeks dislocated and the control group were killed by the same amount of distilled water. Hematoxylin-eosin staining (HEH) and Hoechst_33258 staining were used to detect the changes of hepatocyte apoptosis in alcoholic liver injury in mice. The normal hepatocytes and apoptotic hepatocytes were identified and counted under fluorescence microscope and the apoptosis rate was calculated. Results in the model group, acute liver injury occurred in the third week, and the inflammatory cell infiltration was aggravated in the model group, and in the 8th week, it developed into chronic liver injury, with the appearance of liver tissue watery change and fat vacuole. The apoptotic rate of the model group was 13.20 鹵0.81% and 20.43 鹵0.97% respectively, which was significantly higher than that of the control group (1.38 鹵0.18%). At the end of the 8th week, the apoptotic rate reached 51.99 鹵1.70. The difference was statistically significant compared with the control group (P 0.01). Conclusion the degree of alcoholic liver injury is closely related to the occurrence and development of hepatocyte apoptosis in mice.
【作者單位】： 河南科技大學(xué)醫(yī)學(xué)院肝損傷與修復(fù)分子醫(yī)學(xué)重點實驗室;
【基金】：國家自然科學(xué)基金資助項目(81201558) 河南省高�？萍紕�(chuàng)新團(tuán)隊基金資助項目(181RTSTHN026) 河南省科技攻關(guān)基金資助項目(172102310693) 河南科技大學(xué)創(chuàng)新團(tuán)隊基金資助項目(2015XTD003)
【分類號】：R575

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