本文選題：七味白術(shù)散 + 超微中藥��； 參考：《湖南中醫(yī)藥大學(xué)》2014年碩士論文

【摘要】：目的：研究超微七味白術(shù)散與酵母菌配伍治療菌群失調(diào)腹瀉小鼠的效果,明確七味白術(shù)散對(duì)腸道酵母菌生長(zhǎng)的影響,為臨床治療和新藥的開(kāi)發(fā)與生產(chǎn)提供實(shí)驗(yàn)依據(jù)。 方法：將70只昆明小鼠隨機(jī)分為7組,以無(wú)菌水做正常組,灌胃頭孢拉定和硫酸慶大霉素混合抗生素建立小鼠菌群失調(diào)腹瀉模型后,分別灌胃給藥七味白術(shù)散50%超微湯藥、50%超微湯藥+12.5%酵母、25%超微湯藥+25%酵母、12.5%超微湯藥+50%酵母及全量酵母菌,治療結(jié)束后,通過(guò)分析小鼠體重的變化、免疫器官指數(shù)、腸道可培養(yǎng)菌群數(shù)及腸道內(nèi)重要酶活性來(lái)評(píng)價(jià)超微七味白術(shù)散與酵母菌配伍治療菌群失調(diào)腹瀉小鼠的最佳配方。 通過(guò)濾紙片法和比濁法測(cè)量七味白術(shù)散單味藥及復(fù)方對(duì)腸道酵母菌生長(zhǎng)的影響。 結(jié)果：超微七味白術(shù)散和酵母菌配伍對(duì)菌群失調(diào)腹瀉小鼠體重和脾臟無(wú)影響,各治療組的胸腺指數(shù)均達(dá)到了正常水平(P0.05)；超微七味白術(shù)散和酵母菌均能促進(jìn)雙歧桿菌和乳酸菌的生長(zhǎng)(P0.01),調(diào)節(jié)微生態(tài)平衡；50%超微湯藥組和25%超微湯藥+25%酵母組的大腸桿菌、細(xì)菌數(shù)恢復(fù)至正常水平(P0.05)。經(jīng)治療后,腸道內(nèi)纖維素酶活性均恢復(fù)至正常組水平(P0.05)；50%超微湯藥+12.5%酵母組和25%超微湯藥+25%酵母組的淀粉酶活性恢復(fù)至正常(P0.05)；木聚糖酶、蛋白酶活性與超微飲片的濃度及酵母菌的量有一定的關(guān)系,但均未達(dá)到正常組水平(P0.05)。 人參和甘草對(duì)腸道酵母菌具有抑制作用,人參的最低抑菌濃度為0.7936g/mL,甘草的最低抑菌濃度為0.3949g/mL；茯苓、白術(shù)、葛根對(duì)腸道酵母菌的生長(zhǎng)具有促進(jìn)作用,且在一定濃度范圍內(nèi),藥物濃度增高對(duì)酵母菌生長(zhǎng)的促進(jìn)作用增大；木香和藿香對(duì)腸道酵母菌未表現(xiàn)出明顯的抑促作用。七味白術(shù)散復(fù)方在低濃度下對(duì)腸道酵母菌產(chǎn)生促進(jìn)作用,七味白術(shù)散超微湯劑的作用效果要優(yōu)于傳統(tǒng)湯劑(P0.01)。 結(jié)論：七味白術(shù)散25%超微湯藥+25%酵母為最佳配伍,治療菌群失調(diào)小鼠腹瀉的效果與超微七味白術(shù)散50%量相當(dāng)。 人參和甘草對(duì)腸道酵母菌有抑制作用,茯苓、白術(shù)、葛根及七味白術(shù)散復(fù)方對(duì)腸道酵母菌的生長(zhǎng)有促進(jìn)作用,且超微藥物的作用效果好于傳統(tǒng)飲片
[Abstract]:Objective: to study the effect of Qiwei Baizhu Powder combined with yeast on the growth of intestinal yeast in mice with dysbacteriosis diarrhea, and to provide experimental basis for clinical treatment and the development and production of new drugs. Methods: 70 Kunming mice were randomly divided into 7 groups and treated with aseptic water as normal group. 50% Atractylodes powder 50% ultramicro soup drug 12.5% yeast 25% ultramicro soup drug 25% yeast 12.5% ultrafine soup drug 50% yeast and whole yeast after treatment, the changes of body weight and immune organ index of mice were analyzed after treatment. The number of culturable bacteria and the activity of important enzymes in intestinal tract were used to evaluate the best formula for the treatment of diarrhea mice with the combination of ultramicro Qiwei Baizhu powder and yeast. The effects of Qiwei Atractylodes powder and its compound on the growth of intestinal yeast were measured by filter paper method and turbidimetry. Results: the combination of Qiwei Baizhu Powder and yeast had no effect on the body weight and spleen of mice with dysbacteriosis and diarrhea. The thymus index of each treatment group reached the normal level (P0.05), the growth of Bifidobacterium and lactic acid bacteria was promoted by Qiwei Baizhu Powder and yeast, and the microecological balance was regulated by Escherichia coli in 50% ultrafine soup group and 25% yeast group. The number of bacteria returned to normal level (P0.05). After treatment, the activity of cellulase in the intestine returned to the normal level. The amylase activity of the 50% ultrafine soup group and 25% yeast group returned to normal P0.05, xylanase, P0.05%, xylanase, and xylanase. The activity of protease was related to the concentration of ultrafine slices and the quantity of yeast, but it did not reach the normal level of P0.05. Ginseng and Glycyrrhiza have inhibitory effect on intestinal yeast, the minimum inhibitory concentration of ginseng is 0.7936 g / mL, the minimum inhibitory concentration of licorice is 0.3949 g / mL; Poria cocos, Atractylodes macrocephala and Pueraria lobata have promoting effect on the growth of intestinal yeast, and within a certain concentration range, The increase of drug concentration increased the growth of Saccharomyces cerevisiae, but the growth of Saccharomyces cerevisiae was not significantly inhibited by Astragalus and patchouli. Qiwei Baizhu Powder compound can promote intestinal yeast at low concentration, and the effect of Qiwei Baizhu Powder ultrafine decoction is better than that of traditional decoction P0.01. Conclusion: Qiwei Baizhu Powder 25% Ultramicro decoction 25% yeast is the best compatibility and the effect of treating diarrhea in mice with dysbacteriosis is equal to 50% of Ultramicro Qiwei Atractylodes Powder. Ginseng and liquorice have inhibitory effect on intestinal yeast. Poria cocos, Atractylodes macrocephala, Pueraria lobata and Qiwei Baizhu Powder can promote the growth of intestinal yeast, and the effect of ultramicro medicine is better than that of traditional medicine.
【學(xué)位授予單位】：湖南中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R574.62

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