發(fā)布時(shí)間：2018-05-12 01:25

  本文選題：潰瘍性結(jié)腸炎 + miR-��； 參考：《中藥新藥與臨床藥理》2015年03期

【摘要】：目的探討miR-155在潰瘍性結(jié)腸炎(UC)中的作用機(jī)制。方法構(gòu)建miR-155高表達(dá)潰瘍性結(jié)腸炎小鼠模型,并進(jìn)一步采用Western blot方法檢測外周血白細(xì)胞轉(zhuǎn)錄調(diào)節(jié)因子-1(EST-1)及Th17相關(guān)炎癥因子蛋白表達(dá)。結(jié)果在UC模型組,miR-155基因轉(zhuǎn)染后小鼠外周血白細(xì)胞中miR-155表達(dá)量較對(duì)照顯著升高,且與UC結(jié)腸炎癥程度呈正相關(guān);UC模型組小鼠外周血白細(xì)胞中Est-1呈低表達(dá),而Th17相關(guān)細(xì)胞因子(IL-17、IL-23及IL-6)呈相對(duì)高表達(dá);miR-155轉(zhuǎn)染后,UC小鼠這種變化趨勢更加顯著,Est-1表達(dá)更低,而IL-17、IL-23及IL-6表達(dá)更高。結(jié)論 miR-155通過抑制Ets-1表達(dá),進(jìn)而促進(jìn)Th17相關(guān)炎癥因子分泌參與潰瘍性結(jié)腸炎的發(fā)病。
[Abstract]:Objective to investigate the mechanism of miR-155 in ulcerative colitis (UC). Methods the mouse model of ulcerative colitis with high expression of miR-155 was established, and the expression of transcription regulatory factor -1 (EST-1) and Th17 related inflammatory factor protein (Th17) in peripheral blood leukocytes were detected by Western blot method. Results the expression of miR-155 in peripheral blood leukocytes of UC model group was significantly higher than that of control group, and it was positively correlated with the degree of UC colitis. The expression of Est-1 in peripheral blood leukocytes of UC model group was lower than that of control group. However, the expression of IL-17 IL-23 and IL-6) in Th17 related cytokines was significantly lower in UCU mice after transfection with miR-155, but the expression of IL-17, IL-23 and IL-6 was higher in UCU mice. Conclusion miR-155 inhibits the expression of Ets-1 and promotes the secretion of Th17 related inflammatory factors in the pathogenesis of ulcerative colitis.
【作者單位】： 廣州中醫(yī)藥大學(xué)第一附屬醫(yī)院;
【基金】：廣東省科技計(jì)劃項(xiàng)目(2012B031800483)
【分類號(hào)】：R574.62

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 周鵬志;劉鳳斌;羅琦;孫嫣;丁飛躍;陳斌;;白頭翁湯對(duì)潰瘍性結(jié)腸炎小鼠腸道m(xù)iR-19a表達(dá)的影響[J];南方醫(yī)科大學(xué)學(xué)報(bào);2012年11期

2 周鵬志;陳斌;胡品津;孫嫣;;miR-19a對(duì)潰瘍性結(jié)腸炎的作用機(jī)制[J];南方醫(yī)科大學(xué)學(xué)報(bào);2013年09期

【共引文獻(xiàn)】

相關(guān)期刊論文 前6條

1 周鵬志;陳斌;胡品津;孫嫣;;miR-19a對(duì)潰瘍性結(jié)腸炎的作用機(jī)制[J];南方醫(yī)科大學(xué)學(xué)報(bào);2013年09期

2 李桂森;;白頭翁湯在潰瘍性結(jié)腸炎治療中的應(yīng)用分析[J];中國繼續(xù)醫(yī)學(xué)教育;2015年10期

3 袁恩;;白頭翁湯加味治療潰瘍性結(jié)腸炎的療效及作用機(jī)制[J];現(xiàn)代中西醫(yī)結(jié)合雜志;2014年09期

4 王立娟;劉旭;張亞利;唐志鵬;;仲景方治療潰瘍性結(jié)腸炎研究進(jìn)展[J];中國中醫(yī)藥信息雜志;2014年07期

5 黃艷;竇傳字;劉慧榮;吳璐一;陸Z，

本文編號(hào)：1876508