發(fā)布時(shí)間：2018-04-26 07:31

  本文選題：HBx + HL-7702��； 參考：《福建醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：探討HBx基因?qū)L-7702細(xì)胞增殖的作用，研究分析IL-10對(duì)穩(wěn)定轉(zhuǎn)染HBx基因的HL-7702細(xì)胞增殖的作用以及其與CDKN1B mRNA表達(dá)的關(guān)系。 方法：體外培養(yǎng)HL-7702細(xì)胞、HL-7702/MOCK細(xì)胞與HL-7702/HBx細(xì)胞；CCK8法測(cè)定HBx基因?qū)L-7702細(xì)胞增殖的作用及IL-10對(duì)穩(wěn)定轉(zhuǎn)染HBx基因的HL-7702細(xì)胞增殖的作用；流式細(xì)胞術(shù)測(cè)定IL-10對(duì)HL-7702/HBx細(xì)胞凋亡的影響；RT-PCR法測(cè)定HBx基因的對(duì)HL-7702細(xì)胞表達(dá)CDKN1B mRNA的作用及IL-10對(duì)HL-7702/HBx表達(dá)CDKN1B mRNA的作用。 結(jié)果： CCK8法顯示HL-7702/HBx細(xì)胞比HL-7702細(xì)胞和HL-7702/MOCK細(xì)胞增殖速度明顯加快，，HL-7702細(xì)胞和HL-7702/MOCK細(xì)胞增殖無差別；80ng/ml IL-10作用24小時(shí)可抑制HL-7702/HBx細(xì)胞增殖，而對(duì)HL-7702細(xì)胞、HL-7702/MOCK細(xì)胞增殖無影響；80ng/ml IL-10作用24小時(shí)對(duì)HL-7702細(xì)胞、HL-7702/HBx細(xì)胞、HL-7702/MOCK細(xì)胞凋亡均無影響；HL-7702/HBx細(xì)胞CDKN1B mRNA表達(dá)量較HL-7702細(xì)胞和HL-7702/MOCK細(xì)胞降低，而HL-7702細(xì)胞和HL-7702/MOCK細(xì)胞CDKN1BmRNA表達(dá)量無差別；80ng/ml IL-10作用24小時(shí)后HL-7702/HBx細(xì)胞較HL-7702/HBx空白組CDKN1B mRNA表達(dá)量上調(diào)。 結(jié)論：HBx基因可促進(jìn)HL-7702細(xì)胞增殖，IL-10可抑制HL-7702/HBx細(xì)胞增殖而對(duì)其凋亡無影響，HBx基因可能通過下調(diào)HL-7702/HBx細(xì)胞CDKN1B mRNA表達(dá)量而促進(jìn)細(xì)胞增殖，IL-10可能通過上調(diào)HL-7702/HBx細(xì)胞CDKN1B mRNA表達(dá)量而抑制細(xì)胞增殖。
[Abstract]:Aim: to investigate the effect of HBx gene on the proliferation of HL-7702 cells, and to investigate the effect of IL-10 on the proliferation of HL-7702 cells stably transfected with HBx gene and its relationship with the expression of CDKN1B mRNA. Methods: the effect of HBx gene on the proliferation of HL-7702 cells and the effect of IL-10 on the proliferation of HL-7702 cells stably transfected with HBx gene were determined by HL-7702 cell line HL-7702 / mock and HL-7702/HBx cell line CCK8 in vitro. The effect of IL-10 on apoptosis of HL-7702/HBx cells was determined by flow cytometry. The effect of HBx gene on CDKN1B mRNA expression in HL-7702 cells and the effect of IL-10 on HL-7702/HBx CDKN1B mRNA expression were determined by reverse transcription-polymerase chain reaction (RT-PCR). Results: CCK8 assay showed that the proliferation rate of HL-7702/HBx cells was significantly faster than that of HL-7702 cells and HL-7702/MOCK cells. The proliferation of HL-7702 cells and HL-7702/MOCK cells was similar to that of HL-7702/MOCK cells. The proliferation of HL-7702/HBx cells was inhibited by 80 ng / ml IL-10 for 24 hours. However, there was no effect on the proliferation of HL-7702 / mock cells in HL-7702 cells. The expression of CDKN1B mRNA in HL-7702 / mock cells was significantly lower than that in HL-7702 cells and HL-7702/MOCK cells after 24 hours of exposure to 80 ng / ml IL-10. The apoptosis of HL-7702 / MoCK cells in HL-7702 / HL-7702 / HBX cells was not affected by HL-7702 / Mock cells. However, there was no difference in the expression of CDKN1BmRNA between HL-7702 cells and HL-7702/MOCK cells. After 24 hours of treatment with 80 ng / ml IL-10, the expression of CDKN1B mRNA in HL-7702/HBx cells was higher than that in HL-7702/HBx control group. Conclusion the cell proliferation of HL-7702 cells can be promoted by 1: HBX gene. IL-10 can inhibit the proliferation of HL-7702/HBx cells, but it has no effect on the apoptosis of HL-7702/HBx cells. It may promote the proliferation of HL-7702/HBx cells by down-regulating the expression of CDKN1B mRNA. IL-10 may inhibit the proliferation of HL-7702/HBx cells by upregulating the expression of CDKN1B mRNA.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R512.62

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