本文選題：臍帶 + 間質干細胞移植�。� 參考：《中國組織工程研究》2017年01期

【摘要】：背景:研究表明,臍帶間充質干細胞可以誘導分化成肝樣細胞,從而對肝損傷有一定的修復作用。目的:探討�；撬崤c人臍帶間充質干細胞移植聯(lián)合治療對肝損傷的修復作用。方法:48只健康SD大鼠給予56度白酒灌胃30 d建立慢性酒精性肝損傷模型,建模后將其隨機分為模型組,臍帶間充質干細胞組及�；撬�+臍帶間充質干細胞組。模型組門靜脈注射生理鹽水1 m L,臍帶間充質干細胞組門靜脈移植1 mL臍帶間充質干細胞懸液,�；撬�+臍帶間充質干細胞組門靜脈注射�；撬�0.28 g/L+1 m L臍帶間充質干細胞懸液。移植后2周,檢測各組大鼠血清超氧化物歧化酶、谷胱甘肽過氧化物酶、過氧化氫酶、丙氨酸氨基轉移酶以及丙二醛、內毒素、白細胞介素4水平;采用RT-PCR方法檢測肝細胞特征性功能蛋白CYP2B1的mRNA表達。結果與結論:(1)與模型組相比較,臍帶間充質干細胞組、�；撬�+臍帶間充質干細胞組超氧化物歧化酶、谷胱甘肽過氧化物酶、過氧化氫酶、谷丙轉氨酶水平均顯著增高(P0.05),丙二醛、內毒素、白細胞介素4水平均顯著下降(P0.05)。與臍帶間充質干細胞組比較,牛磺酸+臍帶間充質干細胞組超氧化物歧化酶、谷胱甘肽過氧化物酶、過氧化氫酶、谷丙轉氨酶水平進一步增高(P0.05),丙二醛、內毒素、白細胞介素4水平進一步下降(P0.05);(2)與模型組、臍帶間充質干細胞組比較,肝細胞特征性功能蛋白CYP2B1的mRNA表達在�；撬�+臍帶間充質干細胞組顯著增高,差異有顯著性意義(P0.05);(3)以上實驗結果表明,臍帶間充質干細胞移植可以修復大鼠肝損傷,與�；撬崧�(lián)合使用后效果更佳,其可能與減少自由基對機體的損害有關。
[Abstract]:Background: umbilical cord mesenchymal stem cells can induce hepatoid cells and repair liver injury. Objective: to investigate the repair effect of taurine combined with human umbilical cord mesenchymal stem cell transplantation on liver injury. Methods 48 healthy Sprague-Dawley rats were given 56 degrees liquor for 30 days to establish the model of chronic alcoholic liver injury. The model group was randomly divided into three groups: umbilical cord mesenchymal stem cell group and taurine umbilical cord mesenchymal stem cell group. In model group, 1 mL of umbilical cord mesenchymal stem cell suspension was injected into portal vein and 1 mL of umbilical cord mesenchymal stem cell was injected into portal vein of model group and umbilical cord mesenchymal stem cell group. Mesenchymal stem cells were injected with taurine 0.28 g / L -1 mL umbilical cord mesenchymal stem cell suspension via portal vein. The levels of serum superoxide dismutase, glutathione peroxidase, catalase, alanine aminotransferase, malondialdehyde, endotoxin and interleukin-4 were measured 2 weeks after transplantation. The mRNA expression of CYP2B1 was detected by RT-PCR. Results compared with the model group, superoxide dismutase (SOD), glutathione peroxidase (GSH) and catalase were found in the cord mesenchymal stem cell group and taurine cord mesenchymal stem cell group. The levels of glutamate-alanine aminotransferase (alt) increased significantly (P 0.05), and the levels of malondialdehyde (MDA), endotoxin (LPS) and interleukin 4 (IL-4) decreased significantly (P 0.05). Compared with the umbilical cord mesenchymal stem cells group, the levels of superoxide dismutase, glutathione peroxidase, catalase and glutamate-alanine aminotransferase in taurine cord mesenchymal stem cell group were further increased. Compared with model group and umbilical cord mesenchymal stem cell group, the mRNA expression of hepatocyte characteristic protein CYP2B1 was significantly increased in taurine umbilical cord mesenchymal stem cell group. The results showed that the transplantation of umbilical cord mesenchymal stem cells could repair liver injury in rats, and the effect of combined treatment with taurine was better, which might be related to reducing the damage of free radicals to the body.
【作者單位】： 河北省遵化市人民醫(yī)院外一科;河北省遵化市人民醫(yī)院病理科;
【分類號】：R575

【參考文獻】

相關期刊論文 前10條

1 楊曉蕾;陳智聰;廖繼東;谷景義;于波;劉革修;;尼克酰胺對人臍帶間充質干細胞的保護效應[J];中國病理生理雜志;2015年10期

2 周璐;姚廣濤;曹智麗;徐婷婷;金若敏;;吳茱萸水煎液致小鼠肝毒性機制研究[J];中國實驗方劑學雜志;2013年22期

3 王加志;劉樹民;湯青;李偉;;黃藥子毒性組分對肝細胞內Ca~(2+)濃度的影響[J];中醫(yī)藥信息;2012年06期

4 姚金成;劉穎;胡領;張陸勇;饒健;曾令貴;江振洲;何玲;;雷公藤甲素對人肝細胞L-02肝藥酶活性的影響[J];中國醫(yī)院藥學雜志;2012年20期

5 唐宋琪;楊雪;丁若蘭;岑國棟;高永翔;;雷公藤多苷致大鼠肝損傷的“時-毒”關系及免疫毒性機制研究[J];中藥藥理與臨床;2012年05期

6 徐英;陳崇崇;楊莉;王君明;季莉莉;王崢濤;胡之璧;;基于膽汁酸代謝網(wǎng)絡分析中藥黃藥子的肝毒性[J];藥學學報;2011年01期

7 劉若囡;徐立;時樂;李s，

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