本文選題：促腎上腺皮質(zhì)激素釋放因子 + 腸黏膜屏障　； 參考：《山東大學學報(醫(yī)學版)》2017年04期

【摘要】：目的探討促腎上腺皮質(zhì)激素釋放因子(CRF)對腸道杯狀細胞分泌產(chǎn)物的影響。方法首先用不同濃度的CRF作用于LS174T細胞以確定最佳作用濃度;用10~(-10)mol/L的CRF作用于LS174T細胞,利用Real-time q PCR、Western blotting、免疫熒光染色和ELISA技術(shù)對腸道杯狀細胞分泌產(chǎn)物的表達量進行檢測。結(jié)果 Realtime q PCR結(jié)果顯示,與PBS對照組相比,CRF作用6 h后,黏蛋白2(M UC2),巖藻糖基轉(zhuǎn)移酶-2(FUT2)和三葉因子-3(TFF3)的mRNA表達量均下降(P0.05)。細胞免疫熒光和ELISA的結(jié)果顯示,CRF作用6 h后,MUC2的蛋白表達量降低(P0.05)。Western blotting結(jié)果顯示,CRF作用24 h后,FUT2和TFF3的蛋白表達水平均下降(P0.05)。結(jié)論 CRF可以通過改變LS174T細胞分泌產(chǎn)物的表達,從而破壞腸黏膜屏障功能。
[Abstract]:Objective to investigate the effect of corticotropin releasing factor (CRF) on the secretion of goblet cells. Methods at first, LS174T cells were treated with different concentrations of CRF to determine the optimal concentration, and LS174T cells were treated with 10~(-10)mol/L CRF. The expression of secretory products of intestinal goblet cells was detected by Real-time Q PCR Western blotting, immunofluorescence staining and ELISA technique. Results the results of Realtime Q PCR showed that compared with the control group of PBS, the mRNA expression of mucin 2M UC2P, fucosyltransferase -2FUT2 and trefoil factor -3tf3) decreased after 6 h of treatment with PBS for 6 h. The results of cellular immunofluorescence and ELISA showed that the protein expression of MUC2 decreased after 6 h of treatment. The results of Western blotting showed that the protein expression of FUT2 and TFF3 decreased after 24 h. Conclusion CRF can destroy the intestinal mucosal barrier function by changing the expression of secreted products of LS174T cells.
【作者單位】： 山東大學齊魯醫(yī)院消化內(nèi)科;
【基金】：國家自然科學基金(81330012,81300284,81370495,81400597)
【分類號】：R574

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