本文選題：肝炎 + 乙型��； 參考：《中國現(xiàn)代醫(yī)學(xué)雜志》2015年17期

【摘要】：目的探討乙肝病毒(HBV)感染后不同臨床類型乙型肝炎患者及HBV DNA載量與外周血T細(xì)胞亞群的關(guān)系。方法選取確診的共193例急性乙型肝炎、慢性乙型肝炎、慢性重型肝炎、亞急性重型肝炎、急性重型肝炎、乙肝肝硬化、乙肝肝癌、HBV攜帶者的外周血,流式細(xì)胞儀檢測T細(xì)胞亞群,熒光定量PCR法檢測HBV DNA水平。結(jié)果對照組CD3+、CD4+細(xì)胞百分率、CD4+/CD8+細(xì)胞比值明顯高于各臨床類型組,隨病情加重CD3+、CD4+細(xì)胞百分率、CD4+/CD8+細(xì)胞比值下降明顯,除急性乙型肝炎組和慢性肝炎組CD4+細(xì)胞百分率外與各組比較差異有統(tǒng)計學(xué)意義(P0.05及P0.01);對照組CD8+細(xì)胞百分率、CD4+/CD25+細(xì)胞比值明顯低于各臨床類型組,隨病情加重CD8+細(xì)胞百分率、CD4+/CD25+細(xì)胞比值升高明顯,各組比較差異有統(tǒng)計學(xué)意義(P0.05及P0.01)。低水平HBV DNA載量組(≤103 Copies/ml)CD3+、CD4+細(xì)胞百分率明顯高于其他各病毒組,隨病毒載量增加CD3+、CD4+細(xì)胞百分率逐漸減少,與各組比較差異有統(tǒng)計學(xué)意義(P0.05及P0.01);低水平HBV DNA載量組CD8+細(xì)胞百分率最低,隨病毒載量增加CD8+細(xì)胞百分率升高明顯,與各組比較差異有統(tǒng)計學(xué)意義(P0.05及P0.01);低水平HBV DNA載量組CD4+/CD8+及CD4+/CD25+細(xì)胞比值最高,其比值隨病毒載量增加下降明顯,低水平組CD4+/CD25+細(xì)胞比值與各組比較差異有統(tǒng)計學(xué)意義(P0.05及P0.01),低水平組CD4+/CD8+細(xì)胞比值與各組(103~105組除外)比較差異有統(tǒng)計學(xué)意義(P0.05及P0.01)。除乙肝肝硬化的CD4+細(xì)胞百分率外,同病種肝病的HBV DNA(+)組CD3+、CD4+、CD8+細(xì)胞百分率、CD4+/CD8+及CD4+/CD25+細(xì)胞比值與HBV DNA(-)組比較,差異有統(tǒng)計學(xué)意義(P0.05及P0.01)。結(jié)論細(xì)胞免疫功能紊亂是HBV感染后發(fā)病機制之一,高水平HBV DNA載量可能誘導(dǎo)和加重細(xì)胞免疫功能紊亂,CD4+/CD8+及CD4+/CD25+比值變化可為了解病情變化和指導(dǎo)治療提供依據(jù)。
[Abstract]:Objective to investigate the relationship between HBV DNA load and T cell subsets in peripheral blood of patients with hepatitis B virus (HBV) infection.Methods Peripheral blood samples of 193 patients with acute hepatitis B, chronic hepatitis B, chronic severe hepatitis, subacute severe hepatitis, acute severe hepatitis, liver cirrhosis and liver cancer were selected.T cell subsets were detected by flow cytometry and HBV DNA levels were detected by fluorescence quantitative PCR.Results the percentage of CD4 / CD8 cells of CD3 in the control group was significantly higher than that of each clinical type group, and the percentage of CD4 / CD8 cells in CD3 group decreased significantly with the exacerbation of the disease.The percentage of CD4 cells in acute hepatitis B group and chronic hepatitis group was significantly higher than that in each group (P 0.05 and P 0.01), and the percentage of CD8 cell in control group was significantly lower than that in each clinical type group, and the ratio of CD4 / CD25 cells in control group was significantly lower than that in each clinical type group.The percentage of CD8 cells and the ratio of CD4 / CD25 cells increased significantly with the exacerbation of the disease, and the differences were statistically significant (P 0.05) and P 0.01 (P < 0.05).The percentage of CD4 cells in low level HBV DNA group (鈮，

本文編號：1765342