本文選題：青蒿琥酯 + 非酒精性脂肪性肝病�。� 參考：《安徽醫(yī)科大學學報》2017年10期

【摘要】：目的觀察青蒿琥酯對非酒精性脂肪肝病(NAFLD)小鼠肝臟Toll樣受體4(TLR4)、髓樣分化因子88(My D88)和磷脂酰肌醇-3激酶(PI3K)表達的影響。方法 70只昆明小鼠隨機分為正常對照組、模型組、青蒿琥酯高[60 mg/(kg·d)]、中[30 mg/(kg·d)]、低[15 mg/(kg·d)]劑量組。采用高脂飼料構建NAFLD小鼠動物模型,于給藥后12周末處死,觀察肝臟病理變化,酶法檢測血清三酰甘油(TG)、總膽固醇(TC)、丙氨酸氨基轉移酶(ALT)水平,RT-PCR法檢測肝組織TLR4、My D88、PI3K mRNA表達。結果各青蒿琥酯劑量組小鼠血清TG、TC和ALT水平比模型組顯著降低,肝臟脂肪變性明顯減輕,肝組織TLR4、My D88及PI3K表達量也顯著降低(P0.01,P0.05)。與正常對照組相比,模型組肝組織TLR4、My D88及PI3K表達量顯著升高(P0.01),且三者有相同的變化趨勢。結論 TLR4/My D88及其下游PI3K/AKT通路可能在NAFLD發(fā)病中起重要作用。青蒿琥酯可通過降低TLR4、My D88、PI3K mRNA表達,減輕NAFLD小鼠肝臟脂肪變性程度,降低血脂,改善肝功能指標。
[Abstract]:Objective to observe the effects of artesunate on the expression of Toll like receptor 4TLR4 (myeloid differentiation factor 88(My D88) and phosphatidylinositol 3 kinase (PI3K) in the liver of mice with nonalcoholic fatty liver disease (NAF LDD).Methods 70 Kunming mice were randomly divided into normal control group, model group, artesunate high [60 mg/(kg d], middle [30 mg/(kg d], low [15 mg/(kg d] group.The NAFLD mouse model was established with high fat diet. The liver pathological changes were observed at the end of 12 weeks after administration. The levels of serum triglyceride, total cholesterol and alanine aminotransferase (alt) were detected by enzyme method. The expression of TLR4My D8PI3K mRNA in liver tissue was detected by RT-PCR.Results compared with the model group, the levels of serum TC and ALT in each artesunate group were significantly lower than those in the model group, and the liver steatosis was significantly reduced. The expression of TLR4My D88 and PI3K in the liver tissue was also significantly decreased compared with the model group.Compared with the normal control group, the expression of TLR4My D88 and PI3K in the model group was significantly higher than that in the normal control group.Conclusion TLR4/My D 88 and its downstream PI3K/AKT pathway may play an important role in the pathogenesis of NAFLD.Artesunate could reduce the degree of hepatic steatosis, decrease blood lipid and improve liver function index by decreasing the expression of TLR4My D88PI3K mRNA in liver of NAFLD mice.
【作者單位】： 桂林醫(yī)學院附屬醫(yī)院研究生科;桂林醫(yī)學院附屬醫(yī)院感染性疾病科;桂林醫(yī)學院附屬醫(yī)院新生兒科;桂林醫(yī)學院附屬醫(yī)院老年內(nèi)科;
【基金】：國家自然科學基金(編號:81260078) 廣西自然科學基金(編號:2012GXNSFAA276038、2014GXNSFAA118152) 廣西醫(yī)藥衛(wèi)生自籌經(jīng)費計劃課題(編號:Z2015367)
【分類號】：R575.5

【相似文獻】

中國期刊全文數(shù)據(jù)庫 前3條

1 陳晶;李麗;葉月芳;;青蒿琥酯對非酒精性脂肪炎大鼠肝組織中過氧化物酶體增殖物激活受體γ和固醇調(diào)節(jié)元件結合蛋白-1c表達的影響[J];中國醫(yī)藥導報;2014年23期

2 孫明祥,蔣健,陸廣益,林光居,崔金鳳,吳巧珍,李勁松;高危人群口服青蒿琥酯預防血吸蟲病感染的現(xiàn)場觀察[J];安徽預防醫(yī)學雜志;2000年06期

3 張紹基,林丹丹,劉躍民,劉紅云,劉志德,胡林生,高祖祿,胡飛,徐明生,易志輝,吳玲娟,李思溫;青蒿琥酯預防日本血吸蟲病臨床試驗[J];現(xiàn)代診斷與治療;2000年02期

中國碩士學位論文全文數(shù)據(jù)庫 前2條

1 戴容娟;青蒿琥酯抑制丙型肝炎病毒復制的實驗研究[D];中南大學;2014年

2 閻紫菲;青蒿琥酯對急性胰腺炎動物模型的治療作用及機制研究[D];第三軍醫(yī)大學;2014年

，

本文編號：1737915