本文選題：促肝細(xì)胞生長(zhǎng)素　切入點(diǎn)：活性分析　出處：《南開大學(xué)學(xué)報(bào)(自然科學(xué)版)》2017年02期

【摘要】：采用膜過(guò)濾結(jié)合層析法從豬胎肝中提取純化低分子量促肝細(xì)胞生長(zhǎng)素,并采用MTT法,測(cè)定促肝細(xì)胞生長(zhǎng)素對(duì)人肝癌細(xì)胞Hp G2的增殖影響,結(jié)果表明,8 k Da小肽在1.5和15μg/m L時(shí)可有效促進(jìn)Hp G2的增殖,當(dāng)濃度達(dá)到150μg/m L時(shí),可抑制Hp G2的生長(zhǎng),該研究為肝病營(yíng)養(yǎng)功能組件提供理論與技術(shù)基礎(chǔ),也為促細(xì)胞生長(zhǎng)素的臨床應(yīng)用提供理論依據(jù).
[Abstract]:Low molecular weight hepatocyte growth-promoting hormone (LMWHGF) was extracted and purified from porcine fetal liver by membrane filtration and chromatography. The effect of hepatocyte growth factor on the proliferation of human hepatoma cell line HpG2 was determined by MTT assay.The results showed that 8 kDa peptide could effectively promote the proliferation of HP G2 at 1.5 渭 g / mL and 15 渭 g / mL, and inhibit the growth of HP G2 when the concentration reached 150 渭 g / mL. This study provided a theoretical and technical basis for the nutritional functional components of liver diseases.It also provides a theoretical basis for the clinical application of auxin.
【作者單位】： 北京(中馭)生物工程有限公司;
【基金】：國(guó)家高新技術(shù)研究發(fā)展計(jì)劃(863計(jì)劃)(2014AA022209)
【分類號(hào)】：R512.62;R575

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本文編號(hào)：1730315