本文選題：乙型肝炎病毒　切入點(diǎn)：乙型肝炎X蛋白　出處：《中南大學(xué)》2014年碩士論文

【摘要】：背景和目的：慢性乙型肝炎病毒(HBV)感染被認(rèn)為是肝細(xì)胞癌發(fā)生發(fā)展的主要原因,其中乙肝病毒X蛋白(HBx)在其中扮演著重要的角色。前期的研究表明肝脂肪變性是肝細(xì)胞癌的一個(gè)重要風(fēng)險(xiǎn)因素,HBx可誘導(dǎo)肝脂肪變性。然而,肝臟脂肪酸結(jié)合蛋白(L-FABP)是肝細(xì)胞中脂質(zhì)信號(hào)傳導(dǎo)及脂代謝中重要的脂質(zhì)信號(hào)轉(zhuǎn)導(dǎo)蛋白。而HBx與L-FABP的相關(guān)性及L-FABP在肝細(xì)胞癌發(fā)生發(fā)展的作用及其機(jī)制尚未明了。在本研究中,我們研究了HBx和L-FABP的相關(guān)性及FABP在乙肝病毒誘導(dǎo)的肝癌中的作用機(jī)理。 方法：我們將編碼HBx質(zhì)粒轉(zhuǎn)染人肝癌細(xì)胞株HepG2細(xì)胞,用蛋白免疫印跡法檢測(cè)L-FABP表達(dá)。隨后,轉(zhuǎn)染L-FABP RNAi質(zhì)粒阻止L-FABP的表達(dá),用油紅O染色比較轉(zhuǎn)染L-FABP沉默質(zhì)粒及未轉(zhuǎn)染細(xì)胞內(nèi)脂質(zhì)變性的差異,MTT及DAPI染色檢測(cè)細(xì)胞增殖能力,流式細(xì)胞術(shù)檢測(cè)細(xì)胞周期和細(xì)胞凋亡。 結(jié)果：免疫蛋白印跡結(jié)果顯示轉(zhuǎn)染HBx的細(xì)胞L-FABP的表達(dá)高于未轉(zhuǎn)染細(xì)胞,油紅O染色結(jié)果顯示轉(zhuǎn)染L-FABP RNAi質(zhì)粒細(xì)胞內(nèi)脂滴生成明顯少于未轉(zhuǎn)染細(xì)胞,MTT及DAPI染色顯示轉(zhuǎn)染L-FABP RNAi質(zhì)粒的細(xì)胞增殖能力顯著低于對(duì)照組。 結(jié)論：我們的研究結(jié)果顯示,HBx可以上調(diào)L-FABP的表達(dá),L-FABP在促進(jìn)脂肪的生成的同時(shí),可通過促進(jìn)細(xì)胞增殖和抑制細(xì)胞凋亡的途徑影響肝細(xì)胞癌的發(fā)生發(fā)展。圖12幅,表3個(gè),參考文獻(xiàn)69篇
[Abstract]:Background & objective: chronic hepatitis B virus (HBV) infection is considered to be the main cause of the development of hepatocellular carcinoma, in which hepatitis B virus X protein (HBX) plays an important role.Previous studies have shown that hepatic steatosis is an important risk factor for hepatocellular carcinoma.However, liver fatty acid binding protein (L-FABP) is an important lipid signal transduction protein in hepatocyte lipid signal transduction and lipid metabolism.However, the relationship between HBx and L-FABP, the role of L-FABP in the development of hepatocellular carcinoma and its mechanism are not clear.In this study, we studied the correlation between HBx and L-FABP and the mechanism of FABP in Hepatitis B induced liver cancer.Methods: human hepatoma cell line HepG2 cells were transfected with encoding HBx plasmid and the expression of L-FABP was detected by Western blot.Then, the expression of L-FABP was blocked by transfection of L-FABP RNAi plasmid. The difference between transfected L-FABP silencing plasmid and untransfected cell lipids denaturation was compared by oil red O staining. Cell proliferation ability was detected by DAPI staining, cell cycle and apoptosis were detected by flow cytometry.Results: the results of Western blot showed that the expression of L-FABP in HBx transfected cells was higher than that in untransfected cells.The results of oil red O staining showed that lipid droplet formation in transfected L-FABP RNAi plasmid was significantly lower than that in untransfected cells. DAPI staining showed that the proliferation ability of transfected L-FABP RNAi plasmid was significantly lower than that of control group.Conclusion: our results suggest that HBX can up-regulate the expression of L-FABP and L-FABP may affect the development of hepatocellular carcinoma by promoting cell proliferation and inhibiting apoptosis.12 figures, 3 tables, 69 references
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R512.62;R735.7

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 ;Hepatitis B virus infection and the risk of hepatocellular carcinoma[J];World Journal of Gastroenterology;2011年44期

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本文編號(hào)：1723707