本文選題：重癥急性胰腺炎　切入點：腦損害　出處：《神經(jīng)解剖學雜志》2015年05期

【摘要】：目的:研究P38絲裂原活化蛋白激酶(P38MAPK)對重癥急性胰腺炎(SAP)大鼠海馬神經(jīng)元環(huán)氧合酶-2(COX-2)和前列腺素E2(PGE2)表達的調(diào)控作用。方法:雄性健康SD大鼠36只,體重220~260 g,隨機分為3組。SAP模型組:采用向胰膽管內(nèi)注入5%�；悄懰徕c(2 mg/kg)的方法制備SAP動物模型;抑制劑組:SAP建模成功5 min后,大鼠尾靜脈注射P38MAPK抑制劑SB203580(10 mg/kg);對照組:行剖腹術(shù)翻動胰腺和十二指腸后縫合腹腔。各組大鼠分別于術(shù)后24 h,用Nissl染色和免疫組化法觀察腦組織病理改變的情況,用Western Blot檢測腦組織中p-P38蛋白、COX-2和PGE2的表達情況。結(jié)果:模型組大鼠海馬CA1區(qū)局部錐體細胞缺失,p-P38、COX-2和PGE2陽性細胞數(shù)明顯增加(P0.01),且相應蛋白表達顯著升高(P0.01);SB203580處理組以上變化明顯減輕或不明顯(P0.01)。結(jié)論:P38MAPK對實驗SAP大鼠海馬COX-2和PGE2表達具有顯著調(diào)控作用,而P38MAPK抑制劑SB203580則對SAP大鼠海馬神經(jīng)元具有一定保護作用。
[Abstract]:Aim: to investigate the effects of P38 mitogen-activated protein kinase (P38 MAPK) on the expression of cyclooxygenase-2 (COX-2) and prostaglandin E _ 2 (PGE2) in hippocampal neurons of rats with severe acute pancreatitis (SAP).Methods: 36 healthy male SD rats, weighing 220 ~ 260 g, were randomly divided into 3 groups. SAP model group was established by injecting 5% sodium taurocholate 2 mg / kg into the pancreaticobiliary duct, and the SAP model was established by injection of 5% sodium taurocholate into the pancreatic duct, and 5 min after injection of 5% sodium taurocholate into the pancreatic duct.P38MAPK inhibitor SB203580(10 mg / kg was injected into caudal vein of rats, while in control group, abdominal cavity was sutured after operation of pancreas and duodenum.The pathological changes of brain tissue were observed by Nissl staining and immunohistochemistry at 24 hours after operation. The expression of p-P38 protein COX-2 and PGE2 in brain tissue was detected by Western Blot.Results: the number of COX-2 and PGE2 positive cells in the local pyramidal cells of hippocampal CA1 in the model group was significantly increased, and the corresponding protein expression was significantly increased in the P0.01SSB203580 treated group.Conclusion the expression of COX-2 and PGE2 in hippocampus of experimental SAP rats was significantly regulated by P38 MAPK, while P38MAPK inhibitor SB203580 could protect hippocampal neurons in SAP rats.
【作者單位】： 第四軍醫(yī)大學唐都醫(yī)院消化科;解放軍第323醫(yī)院肝膽外科;第四軍醫(yī)大學西京醫(yī)院消化科;
【基金】：國家自然科學基金(30872965,30971337)
【分類號】：R576

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本文編號：1710337