本文選題：神經(jīng)生長因子　切入點：四氯化碳　出處：《重慶醫(yī)學(xué)》2015年15期

【摘要】：目的觀察神經(jīng)生長因子(NGF)對四氯化碳(CCL4)誘導(dǎo)的小鼠肝纖維化的影響。方法將30只雌性昆明小鼠分成3組:纖維化模型組(A組)、NGF干預(yù)組(B組)和生理鹽水對照組(C組)。8周時采集標(biāo)本,用全自動生化分析儀檢測丙氨酸氨基轉(zhuǎn)移酶(ALT)、天冬氨酸氨基轉(zhuǎn)移酶(AST)、總膽紅素(TBIL)、清蛋白(ALB);放射免疫法檢測肝纖維化指標(biāo):透明質(zhì)酸(HA)、層粘連蛋白(LN)、Ⅲ型前膠原(PCⅢ)。采用Ishaki評分系統(tǒng)對小鼠肝組織炎癥活動度及纖維化程度進行評分。結(jié)果A、B組ALT、AST、HA、LN均明顯高于C組(F=111.45,658.80,157.43,167.99;P0.05);B組ALT、AST、LN均明顯低于A組(P0.05)。HE染色、網(wǎng)狀纖維染色及Masson染色顯示,A組肝組織炎癥及纖維化程度最明顯,B組肝組織炎癥較A組顯著減輕,未形成纖維間隔,纖維組織細(xì)短,C組肝組織未見明顯炎癥細(xì)胞浸潤及纖維形成。A組肝臟炎癥分級和纖維化分期評分較B、C組高,且3組炎癥活動度得分、纖維化得分差異有統(tǒng)計學(xué)意義(均P0.05)。結(jié)論 NGF可以阻斷CCL4誘導(dǎo)的小鼠肝纖維化,減輕肝組織炎癥。
[Abstract]:Objective to observe the effect of NGF on liver fibrosis induced by CCL 4 in mice.Methods Thirty female Kunming mice were divided into three groups: fibrosis model group (group A) and control group (group C).Alanine aminotransferase (alt), aspartate aminotransferase (AST), total bilirubin (TBILL), albumin (ALBN) were detected by automatic biochemical analyzer, and liver fibrosis indexes were detected by radioimmunoassay: hyaluronic acid Ham, laminin (LN), procollagen 鈪，

本文編號：1692939