【摘要】：目的:探討miR-124-3p在鼻咽癌中的表達(dá)及其對(duì)鼻咽癌細(xì)胞的影響及其機(jī)制。方法:收集鼻咽癌組織標(biāo)本90例和慢性鼻咽炎癥組織標(biāo)本85例,運(yùn)用qRT-PCR方法檢測(cè)組織標(biāo)本和CNE1、CNE2、SUNE1、HONE1、5-8F、6-10B及C666-1鼻咽癌細(xì)胞株和永生化鼻咽上皮細(xì)胞NP69中miR-124-3p的表達(dá),脂質(zhì)體介導(dǎo)的轉(zhuǎn)染方法下調(diào)高表達(dá)miRNA-124-3p的鼻咽癌細(xì)胞株CNE2,同時(shí)上調(diào)低表達(dá)miRNA-124-3p的鼻咽癌細(xì)胞株C666-1。CCK8法、流式細(xì)胞技術(shù)、細(xì)胞劃痕實(shí)驗(yàn)、Transwell實(shí)驗(yàn)和Boyden小室檢測(cè)細(xì)胞增殖、凋亡、遷移及侵襲的變化;生物信息學(xué)靶基因預(yù)測(cè)miRNA-124-3的靶基因并運(yùn)用熒光素酶報(bào)告實(shí)驗(yàn)驗(yàn)證,Western Blotting檢測(cè)細(xì)胞中STAT3及其下游的p-STAT3、CCND2和MMP2蛋白表達(dá)水平。結(jié)果:鼻咽癌組織中miRNA-124-3p表達(dá)水平與慢性鼻咽炎癥組織相比明顯下調(diào)(P0.001);miRNA-124-3p的表達(dá)水平與腫瘤大小及范圍、區(qū)域淋巴結(jié)受累情況及臨床分期顯著相關(guān)(均P0.001);7種鼻咽癌細(xì)胞與NP69細(xì)胞相比較,miRNA-124-3p的表達(dá)水平均顯著低于NP69(均P0.05)。miRNA-124-3p過(guò)表達(dá)后C666-1細(xì)胞增殖、凋亡、遷移和侵襲均顯著低于空白對(duì)照組和mimics NC組(均P0.05);miRNA-124-3p抑制后CNE2細(xì)胞的增殖、凋亡、遷移和侵襲均顯著高于inhibitor NC組和空白對(duì)照組(均P0.05)。轉(zhuǎn)染miRNA-124-3p后C666-1細(xì)胞STAT3、p-STAT3、CCND2和MMP2的表達(dá)顯著降低,抑制CNE2細(xì)胞的miRNA-124-3p表達(dá)后STAT3、p-STAT3、CCND2和MMP2的表達(dá)顯著降低。結(jié)論:miR-124-3p可通過(guò)下調(diào)靶基因STAT3的表達(dá),進(jìn)而影響其下游的p-STAT3、CCND2和MMP2信號(hào)通路,從而促進(jìn)鼻咽癌細(xì)胞凋亡,抑制鼻咽癌細(xì)胞的增殖、遷移及侵襲。
[Abstract]:Objective: to investigate the expression of miR-124-3p in nasopharyngeal carcinoma (NPC) and its effect on nasopharyngeal carcinoma (NPC) cells and its mechanism. Methods: 90 cases of nasopharyngeal carcinoma and 85 cases of chronic nasopharyngitis were collected. Tissue specimens and CNE1,CNE2,SUNE1,HONE1,5-8F, were detected by qRT-PCR. The expression of miR-124-3p in 6 / 10 B and C66 鈮，

本文編號(hào)：2479074