【摘要】：目的研究哇巴因?qū)π∈髢?nèi)耳神經(jīng)膠質(zhì)細(xì)胞的影響,為干細(xì)胞移植治療感音神經(jīng)性聾的研究奠定基礎(chǔ)。方法成年雌性SPF級(jí)CBA/J小鼠60只,隨機(jī)分為實(shí)驗(yàn)組和對(duì)照組,每組30只;實(shí)驗(yàn)組動(dòng)物接受哇巴因(3mM)經(jīng)圓窗滲透給藥,對(duì)照組給予等量生理鹽水,于給藥后7、14和30天用免疫組織熒光染色法觀察位于耳蝸螺旋神經(jīng)節(jié)內(nèi)的內(nèi)耳神經(jīng)膠質(zhì)細(xì)胞(inner ear glial cells,IEGs)的變化。結(jié)果給藥后7、14、30天實(shí)驗(yàn)組耳蝸各回螺旋神經(jīng)節(jié)內(nèi)可見內(nèi)耳神經(jīng)膠質(zhì)細(xì)胞存活,但數(shù)量減少,排列紊亂;與對(duì)照組相比,哇巴因給藥后7天實(shí)驗(yàn)組耳蝸各回神經(jīng)膠質(zhì)細(xì)胞的數(shù)量及密度即顯著減少,給藥后14天及30天后膠質(zhì)細(xì)胞數(shù)量進(jìn)一步減少;給藥后30天各回螺旋神經(jīng)節(jié)內(nèi)內(nèi)耳神經(jīng)膠質(zhì)細(xì)胞數(shù)降至最低,與實(shí)驗(yàn)組給藥后7天、14天及與同時(shí)間點(diǎn)對(duì)照組比較顯著減少,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論哇巴因經(jīng)圓窗滲透給藥可直接造成CBA/J小鼠內(nèi)耳螺旋神經(jīng)節(jié)內(nèi)神經(jīng)膠質(zhì)細(xì)胞的急性進(jìn)行性損傷。
[Abstract]:Aim to study the effect of ouabain on glial cells in mouse inner ear, and to establish a foundation for stem cell transplantation in the treatment of sensorineural hearing loss. Methods 60 adult female CBA/J mice of SPF grade were randomly divided into experimental group and control group with 30 in each group. The experimental group was treated with 3mM through the round window, while the control group was given the same amount of normal saline. The changes of (inner ear glial cells,IEGs in the inner ear glial cells located in the cochlear spiral ganglion were observed by immunofluorescence staining on the 14th and 30th day after administration. Results the glial cells in the spiral ganglion of the cochlea were observed in the experimental group, but the number of glial cells was decreased and the arrangement was disordered. Compared with the control group, the number and density of glial cells in the cochlear gyrus of the experimental group decreased significantly 7 days after administration of ouabain, and further decreased on the 14th and 30th day after administration of ouabain. 30 days after administration, the number of glial cells in the inner ear of the spiral ganglion decreased to the lowest, which was significantly lower than that in the experimental group on the 7th day, the 14th day and the control group at the same time point (P0.05). Conclusion ouabain permeated through the round window can directly cause the acute progressive injury of glial cells in the spiral ganglion of the inner ear of CBA/J mice.
【作者單位】： 武漢大學(xué)人民醫(yī)院耳鼻咽喉頭頸外科;
【基金】：國家自然科學(xué)基金面上項(xiàng)目(81271073) 教育部留學(xué)回國基金(302-153775)資助
【分類號(hào)】：R764.43

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