【摘要】：目的研究哇巴因對小鼠內耳神經膠質細胞的影響,為干細胞移植治療感音神經性聾的研究奠定基礎。方法成年雌性SPF級CBA/J小鼠60只,隨機分為實驗組和對照組,每組30只;實驗組動物接受哇巴因(3mM)經圓窗滲透給藥,對照組給予等量生理鹽水,于給藥后7、14和30天用免疫組織熒光染色法觀察位于耳蝸螺旋神經節(jié)內的內耳神經膠質細胞(inner ear glial cells,IEGs)的變化。結果給藥后7、14、30天實驗組耳蝸各回螺旋神經節(jié)內可見內耳神經膠質細胞存活,但數量減少,排列紊亂;與對照組相比,哇巴因給藥后7天實驗組耳蝸各回神經膠質細胞的數量及密度即顯著減少,給藥后14天及30天后膠質細胞數量進一步減少;給藥后30天各回螺旋神經節(jié)內內耳神經膠質細胞數降至最低,與實驗組給藥后7天、14天及與同時間點對照組比較顯著減少,差異有統(tǒng)計學意義(P0.05)。結論哇巴因經圓窗滲透給藥可直接造成CBA/J小鼠內耳螺旋神經節(jié)內神經膠質細胞的急性進行性損傷。
[Abstract]:Aim to study the effect of ouabain on glial cells in mouse inner ear, and to establish a foundation for stem cell transplantation in the treatment of sensorineural hearing loss. Methods 60 adult female CBA/J mice of SPF grade were randomly divided into experimental group and control group with 30 in each group. The experimental group was treated with 3mM through the round window, while the control group was given the same amount of normal saline. The changes of (inner ear glial cells,IEGs in the inner ear glial cells located in the cochlear spiral ganglion were observed by immunofluorescence staining on the 14th and 30th day after administration. Results the glial cells in the spiral ganglion of the cochlea were observed in the experimental group, but the number of glial cells was decreased and the arrangement was disordered. Compared with the control group, the number and density of glial cells in the cochlear gyrus of the experimental group decreased significantly 7 days after administration of ouabain, and further decreased on the 14th and 30th day after administration of ouabain. 30 days after administration, the number of glial cells in the inner ear of the spiral ganglion decreased to the lowest, which was significantly lower than that in the experimental group on the 7th day, the 14th day and the control group at the same time point (P0.05). Conclusion ouabain permeated through the round window can directly cause the acute progressive injury of glial cells in the spiral ganglion of the inner ear of CBA/J mice.
【作者單位】： 武漢大學人民醫(yī)院耳鼻咽喉頭頸外科;
【基金】：國家自然科學基金面上項目(81271073) 教育部留學回國基金(302-153775)資助
【分類號】：R764.43

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本文編號：2406614