【摘要】：目的:研究并探討轉(zhuǎn)移抑素(metastin)對鼻咽癌高轉(zhuǎn)移能力細(xì)胞株SUNE-1-5-8F轉(zhuǎn)移侵襲能力的影響。方法:(1)通過Transwell體外侵襲趨化實(shí)驗技術(shù),測定在不同濃度(102nM,103nM,104nM)的轉(zhuǎn)移抑素干預(yù)前后SUNE-1-5-8F細(xì)胞轉(zhuǎn)移及侵襲細(xì)胞數(shù)目,探究其對SUNE-1-5-8F細(xì)胞轉(zhuǎn)移及侵襲能力的影響。(2)利用BALB/c-nu裸鼠肺轉(zhuǎn)移模型,40只雌性裸鼠隨機(jī)分為轉(zhuǎn)移抑素組及對照組,每組各20只,兩組均在腋窩下注射SUNE-1-5-8F細(xì)胞懸液2×106/ml,其中轉(zhuǎn)移抑素組于注射細(xì)胞后第二天按1.17mg/kg(轉(zhuǎn)移抑素量/裸鼠體重)腹腔注射轉(zhuǎn)移抑素,研究轉(zhuǎn)移抑素對裸鼠移植瘤的抑瘤率、轉(zhuǎn)移抑制率及生命延長率的影響;采用免疫組化法研究裸鼠肺轉(zhuǎn)移灶kisspeptin指標(biāo)表達(dá)情況。結(jié)果:(1)Transwell體外侵襲趨化實(shí)驗中轉(zhuǎn)移抑素對于鼻咽癌高轉(zhuǎn)移能力細(xì)胞SUNE-1-5-8F的轉(zhuǎn)移能力及侵襲能力均具有顯著的抑制作用(P0.05),且隨著轉(zhuǎn)移抑素濃度的提升與其抑制轉(zhuǎn)移侵襲能力呈正相關(guān)(P0.05)。(2)BALB/c-nu裸鼠肺轉(zhuǎn)移模型實(shí)驗中轉(zhuǎn)移抑素對腫瘤的生長即抑瘤率無明顯的影響(P0.05),但可以有效的降低肺轉(zhuǎn)移數(shù),提升其轉(zhuǎn)移抑制率(P0.05),且進(jìn)一步提升了小鼠的生命延長率(P0.05)。免疫組化結(jié)果顯示轉(zhuǎn)移抑素組kisspeptin較對照組高表達(dá)。結(jié)論:(1)在Transwell體外侵襲趨化實(shí)驗中,轉(zhuǎn)移抑素能夠明顯抑制鼻咽癌高轉(zhuǎn)移能力細(xì)胞株SUNE-1-5-8F的轉(zhuǎn)移侵襲能力,且轉(zhuǎn)移抑素濃度與其抑制能力呈正相關(guān)。(2)在BALB/c-nu裸鼠肺轉(zhuǎn)移模型實(shí)驗中,轉(zhuǎn)移抑素能夠有效降低肺轉(zhuǎn)移率,從而延長裸鼠生命,但無法抑制腫瘤的生長。
[Abstract]:Objective: to investigate the effect of metastatic (metastin) on metastasis and invasion of NPC cell line SUNE-1-5-8F. Methods: (1) Transwell invasion chemotactic assay was used to determine the number of SUNE-1-5-8F cell metastasis and invasion cells before and after treatment with different concentrations (102nM) of 103nMU 104nM. To investigate the effect of SUNE-1-5-8F on the metastasis and invasion of SUNE-1-5-8F cells. (2) using the lung metastasis model of BALB/c-nu nude mice, 40 female nude mice were randomly divided into metastatic statin group and control group with 20 in each group. SUNE-1-5-8F cell suspension was injected into the axilla of both groups (2 脳 10 6 / ml). The metastatic statin group was injected intraperitoneally with 1.17mg/kg on the second day after injection of the cells. To study the effect of metastasis statin on tumor inhibition rate, metastasis inhibition rate and life prolongation rate of xenograft tumor in nude mice. The expression of kisspeptin in lung metastases in nude mice was studied by immunohistochemical method. Results: (1) in vitro invasion and chemotactic assay of Transwell, metastatic statin significantly inhibited the metastasis and invasion of SUNE-1-5-8F cells with high metastatic ability of nasopharyngeal carcinoma (P0.05). And with the increase of the concentration of metastasis, there was a positive correlation between the inhibition of metastasis and invasion ability (P0.05). (2) in the lung metastasis model of BALB/c-nu nude mice. There was no significant effect of metastasis on tumor growth and tumor inhibition rate (P0.05). But can effectively reduce the number of lung metastasis, enhance its metastasis inhibition rate (P0.05), and further enhance the survival rate of mice (P0.05). Immunohistochemical results showed that the expression of kisspeptin in the metastatic statin group was higher than that in the control group. Conclusion: (1) Metastatin can significantly inhibit the metastasis and invasion ability of nasopharyngeal carcinoma cell line SUNE-1-5-8F in vitro invasion and chemotaxis assay. The concentration of metastatic statin was positively correlated with its inhibitory ability. (2) in the lung metastasis model of BALB/c-nu nude mice, metastatic statin could effectively reduce the rate of lung metastasis and prolong the life of nude mice, but could not inhibit the growth of tumor.
【學(xué)位授予單位】：蚌埠醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R739.63

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