【摘要】：目的探究不同強(qiáng)度和時間紫外線照射對大鼠淚膜穩(wěn)定性和角膜組織的影響。設(shè)計(jì)實(shí)驗(yàn)研究。研究對象50只健康SD大鼠(6周,200~250 g)。方法將大鼠隨機(jī)分成5組(空白對照組N和實(shí)驗(yàn)組A、B、C、D),每組10只,A、C組紫外線照射強(qiáng)度為142μw/cm~2,每次照射時長為8、12分鐘;B、D紫外線照射強(qiáng)度為94μw/cm~2,每次照射時長為12、18分鐘。共照射42天,每周照射3次,均間隔1天,照射后第14、28、42天,裂隙燈檢查、熒光素染色及淚膜破裂時間(BUT)測定、Schirmer I試驗(yàn)(SIt)。49天時注射過量水合氯醛處死大鼠,制作角膜組織切片。主要指標(biāo)結(jié)膜、角膜、淚膜形態(tài)結(jié)構(gòu)變化,角膜熒光素染色評分,BUT,SIt值,角膜組織切片觀察。結(jié)果裂隙燈檢查顯示B、C組對比,照射強(qiáng)度較高組比較低組瞼結(jié)膜充血、角膜水腫、角膜混濁出現(xiàn)早且嚴(yán)重,較高強(qiáng)度組照射后出現(xiàn)角膜新生血管,較低強(qiáng)度組以淚液質(zhì)和量改變?yōu)橹饕卣�。A、C組對比,長時間照射組比短時間照射組角膜水腫、混濁和新生血管出現(xiàn)的時間早且嚴(yán)重,且長時間照射組瞼裂區(qū)出現(xiàn)條狀潰瘍。熒光素染色顯示,正常組染色評價結(jié)果為陰性,A組為陽性,B組為弱陽性,C、D組為強(qiáng)陽性。第42天,A、B、C、D組BUT值分別較對照組明顯減少(P均0.01)。第42天,實(shí)驗(yàn)組SIt值分別較對照組均明顯增加(P均0.01)。組織學(xué)觀察顯示,較高強(qiáng)度紫外線照射引起角膜新生血管,內(nèi)皮細(xì)胞密度下降,基質(zhì)層纖維細(xì)胞排列紊亂。較低強(qiáng)度短時間照射引起上皮層增厚。較低強(qiáng)度長時間照射引起角膜新生血管,基質(zhì)層纖維細(xì)胞排列紊亂。結(jié)論長時間紫外線照射可引起瞼結(jié)膜充血、角膜上皮損傷、淚液質(zhì)和量改變等眼表結(jié)構(gòu)變化,這些變化隨照射強(qiáng)度增大或照射時間增長而出現(xiàn)更早、程度更重。
[Abstract]:Objective to investigate the effects of ultraviolet irradiation on tear film stability and corneal tissue in rats. Design experimental research. Participants 50 healthy SD rats (6 weeks, 200,250 g).) Methods the rats were randomly divided into 5 groups (control group N and experimental group Acarinic D) with 10 rats in each group. The ultraviolet radiation intensity was 142 渭 w / cm ~ (-2) in APC group, and the duration of each irradiation was 812 minutes. The intensity of ultraviolet radiation was 94 渭 w / cm ~ (-2), and the duration of each irradiation was 12 ~ (18) minutes. The rats were killed by slit lamp examination, fluorescein staining and lacrimal film rupture time (BUT). The rats were killed by injection of excessive chloral hydrate on day 49 of, Schirmer I test (SIt). 49. Corneal tissue sections were made. Main outcome measures: changes in morphology and structure of conjunctiva, cornea and lacrimal film, corneal fluorescein staining score, BUT,SIt value and corneal tissue sections. Results slit-lamp examination showed that the blepharoconjunctival congestion, corneal edema and corneal opacity appeared earlier and more seriously in the higher radiation intensity group than in the lower group, and corneal neovascularization appeared in the higher intensity group after irradiation. The changes of tear quality and quantity were the main characteristics in the low intensity group. Compared with the control group, the corneal edema, turbidity and neovascularization appeared earlier and more seriously in the long time irradiation group than in the short time irradiation group. In the long-term irradiation group, stripe ulcers appeared in the cleft eyelid region. The results of fluorescein staining were negative in normal group, positive in group A, weak positive in group B and strong positive in group C and D. On the 42nd day, the BUT values of the D group were significantly lower than those of the control group (P 0.01). On the 42 th day, the SIt value of the experimental group was significantly higher than that of the control group (P 0.01). Histopathological observation showed that the corneal neovascularization was induced by high intensity ultraviolet irradiation, the density of endothelial cells decreased, and the fibroblasts of stromal layer were arranged in disorder. The thickening of the epithelium was induced by low intensity and short time irradiation. Low intensity and long time irradiation resulted in corneal neovascularization and disorder in the arrangement of fibroblasts in the stromal layer. Conclusion Ultraviolet irradiation for a long time can cause hyperemia of eyelid conjunctiva, corneal epithelium injury, tear quality and quantity change. These changes appear earlier and more serious with the increase of irradiation intensity or irradiation time.
【作者單位】： 福建醫(yī)科大學(xué)醫(yī)學(xué)技術(shù)與工程學(xué)院眼視光學(xué)系;
【基金】：福建醫(yī)科大學(xué)2015年國家級大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目(201510392026) 福建省自然科學(xué)基金(2016J01155)
【分類號】：R77

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