【摘要】：目的為了探討iNKT細(xì)胞是否參與了分泌性中耳炎的發(fā)生，本文采用流式細(xì)胞術(shù)，對(duì)分泌性中耳炎患兒外周血、扁桃體組織、腺樣體組織內(nèi)iNKT細(xì)胞進(jìn)行檢測(cè)，探討用anti-humanCD3-FITC和anti-human6B11-PE標(biāo)記iNKT細(xì)胞的可行性，首次分析iNKT細(xì)胞與兒童分泌性中耳炎的關(guān)系。 方法分別收集正常兒童外周血10例，不伴SOM的慢性扁桃體炎和腺樣體肥大患兒的外周血、扁桃體、腺樣體組織各13例，以及分泌性中耳炎患兒外周血13例、扁桃體組織7例、腺樣體組織12例。用特異性抗體anti-human CD3-FITC和anti-human6B11-PE標(biāo)記外周血和組織中iNKT細(xì)胞，利用流式細(xì)胞學(xué)術(shù)（FCM）檢測(cè)iNKT細(xì)胞在各標(biāo)本的百分比，并比較各組間是否存在差異。 結(jié)果1外周血iNKT細(xì)胞的檢測(cè)結(jié)果 正常兒童外周血iNKT細(xì)胞的比例為（0.9660±0.4221）%；不伴SOM的慢性扁桃體炎和腺樣體肥大患兒外周血iNKT細(xì)胞的比例為（1.5231±1.0226）%；分泌性中耳炎患兒外周血iNKT細(xì)胞的比例為（1.5692±0.7200）%;經(jīng)單因素的方差分析，計(jì)算得P＜0.05，，三組間的差異有統(tǒng)計(jì)學(xué)意義，經(jīng)LSD兩兩比較，正常兒童與分泌性中耳炎患兒差異顯著，其他兩組間差異無統(tǒng)計(jì)學(xué)意義。 2扁桃體組織iNKT細(xì)胞的檢測(cè)結(jié)果 分泌性中耳炎患兒扁桃體組織iNKT細(xì)胞的比例為（1.0105±0.5241）%；不伴SOM的慢性扁桃體炎患兒扁桃體組織iNKT細(xì)胞的比例為（0.8883±0.6419）%；經(jīng)兩獨(dú)立樣本的t檢驗(yàn)，計(jì)算得P＞0.05，兩組間差異無統(tǒng)計(jì)學(xué)意義。 3腺樣體組織iNKT細(xì)胞檢測(cè)結(jié)果 分泌性中耳炎患兒腺樣體組織iNKT細(xì)胞的比例為（1.2658±0.8250）%；不伴SOM的腺樣體肥大患兒腺樣體組織iNKT細(xì)胞的比例為（0.8246±0.4289）%；經(jīng)兩獨(dú)立樣本t檢驗(yàn)，計(jì)算得P＞0.05，兩組間差異無統(tǒng)計(jì)學(xué)意義。 結(jié)論用anti-human CD3-FITC和anti-human6B11-PE標(biāo)記iNKT細(xì)胞可行。在SOM患兒與不伴SOM的慢性扁桃體炎、腺樣體肥大患兒的扁桃體、腺樣體組織中，iNKT細(xì)胞比例差異無統(tǒng)計(jì)學(xué)意義。分泌性中耳炎患兒外周血中iNKT細(xì)胞的比例比正常兒童高，初步推測(cè)iNKT細(xì)胞與兒童分泌性中耳炎的發(fā)生可能有關(guān)。
[Abstract]:Objective to investigate whether iNKT cells are involved in the occurrence of secretory otitis media, iNKT cells in peripheral blood, tonsils and adenoids of children with secretory otitis media were detected by flow cytometry. To explore the feasibility of labeling iNKT cells with anti-humanCD3-FITC and anti-human6B11-PE, the relationship between iNKT cells and secretory otitis media in children was analyzed for the first time. Methods the peripheral blood, tonsillar and adenoid tissues of 10 normal children, 13 children with chronic tonsillitis and adenoid hypertrophy without SOM, 13 patients with secretory otitis media and 7 patients with tonsillar tissue were collected. Adenoid tissue in 12 cases. Specific antibody anti-human CD3-FITC and anti-human6B11-PE were used to label iNKT cells in peripheral blood and tissues. The percentage of iNKT cells in each specimen was detected by flow cytometry academic (FCM). Results 1 the percentage of iNKT cells in peripheral blood of normal children was (0.9660 鹵0.4221)%, that of children without SOM was (1.5231 鹵1.0226)%, that of children with chronic tonsillitis and adenoid hypertrophy was (1.5231 鹵1.0226)%. The percentage of iNKT cells in peripheral blood of children with secretory otitis media was (1.5692 鹵0.7200)%. By single factor analysis of variance, P < 0.05, the difference among the three groups was statistically significant. By LSD, there was significant difference between normal children and children with secretory otitis media, but there was no significant difference between the other two groups. 2Detection of iNKT cells in tonsils the percentage of iNKT cells in tonsils of children with secretory otitis media was (1.0105 鹵0.5241)%. The percentage of iNKT cells in tonsil tissue of children with chronic tonsillitis without SOM was (0.8883 鹵0.6419)%, which was calculated by t-test of two independent samples (P > 0.05), and there was no significant difference between the two groups. 3The percentage of adenoid tissue iNKT cells was (1.2658 鹵0.8250)% in children with secretory otitis media and (0.8246 鹵0.4289)% in adenoid tissues without SOM. After two independent samples t test, P > 0.05, there was no significant difference between the two groups. Conclusion it is feasible to label iNKT cells with anti-human CD3-FITC and anti-human6B11-PE. There was no significant difference in the proportion of iNKT cells in tonsils and adenoids between children with SOM and those with chronic tonsillitis without SOM and adenoid hypertrophy. The percentage of iNKT cells in peripheral blood of children with secretory otitis media was higher than that of normal children. It was suggested that iNKT cells might be related to the occurrence of secretory otitis media in children.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R764.21

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