【摘要】：目的觀察β趨化因子對小鼠小膠質細胞的活化及誘導感光細胞凋亡的作用。設計實驗性研究。研究對象β趨化因子、小鼠BV-2小膠質細胞系及661w感光細胞系。方法將β趨化因子(RANTES、MIP-1α及MIP-1β)加入到BV-2細胞中,觀察BV-2細胞活化反應。然后將被激活的BV-2細胞培養(yǎng)液上清加入661w細胞中,觀察661w的凋亡情況�；驅ⅵ纶吇蜃又苯蛹尤氲�661w細胞中,觀察其凋亡情況。事先加入β趨化因子抑制劑met-RANTES后,重復上述操作。主要指標鈣內(nèi)流、ROS及NO的產(chǎn)生、TUNEL陽性感光細胞百分比。結果β趨化因子加入BV-2細胞后,細胞內(nèi)鈣流曲線明顯上升、細胞外ROS及NO產(chǎn)生較對照組均明顯增高(P0.01);將激活后的BV-2細胞上清加入到661w細胞中,后者凋亡率增加30%。β趨化因子直接加入661w細胞中,上述指標無明顯變化。在加入β趨化因子之前先加入其抑制劑met-RANTES,BV-2細胞的ROS產(chǎn)生較未加抑制劑組明顯降低(P0.01)、NO產(chǎn)生較未加抑制劑組降低(P=0.0187),661w細胞凋亡明顯減少。結論β趨化因子可活化小鼠小膠質細胞導致感光細胞凋亡。β趨化因子抑制劑可抑制遺傳性視網(wǎng)膜變性小鼠小膠質細胞活化,保護感光細胞。
[Abstract]:Objective to observe the effect of 尾 chemokine on the activation of mouse microglia and the induction of photoreceptor cell apoptosis. Design experimental studies. Object 尾 chemokine, mouse BV-2 microglia cell line and 661w photoreceptor cell line were studied. Methods 尾 chemokines (RANTES,MIP-1 偽 and MIP-1 尾) were added to BV-2 cells to observe the activation of BV-2 cells. Then the supernatant of activated BV-2 cells was added to 661w cells to observe the apoptosis at 661w. Or 尾 chemokine was added directly to 661w cells to observe its apoptosis. After adding 尾 chemokine inhibitor met-RANTES in advance, repeat the above operation. Main outcome measures calcium influx, production of ROS and NO, percentage of TUNEL positive photosensitive cells. Results after the addition of 尾 chemokine to BV-2 cells, the intracellular calcium flow curve increased significantly, and the production of extracellular ROS and NO increased significantly compared with the control group (P0.01). When the activated supernatant of BV-2 cells was added to 661w cells, the apoptotic rate of the latter increased by 30%, and 尾 chemokine was added directly into 661w cells. The ROS production of met-RANTES,BV-2 cells with 尾 chemokine was significantly lower than that of non-inhibitor group (P0.01), NO production was lower than that of non-inhibitor group (P0. 0187), and apoptosis was significantly decreased at 661w. Conclusion 尾 chemokine can activate mouse microglia and induce photoreceptor cell apoptosis. 尾 chemokine inhibitor can inhibit the activation of microglia and protect photoreceptor cells in mice with hereditary retinal degeneration.
【作者單位】： 首都醫(yī)科大學附屬北京同仁醫(yī)院北京同仁眼科中心北京市眼科研究所;眼科學與視覺科學北京市重點實驗室;中國科學院北京基因研究所;
【基金】：國家自然科學基金(81100675) 北京市自然科學基金(7102034)
【分類號】：R774.13
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本文編號：2367374