【摘要】：目的觀察β趨化因子對(duì)小鼠小膠質(zhì)細(xì)胞的活化及誘導(dǎo)感光細(xì)胞凋亡的作用。設(shè)計(jì)實(shí)驗(yàn)性研究。研究對(duì)象β趨化因子、小鼠BV-2小膠質(zhì)細(xì)胞系及661w感光細(xì)胞系。方法將β趨化因子(RANTES、MIP-1α及MIP-1β)加入到BV-2細(xì)胞中,觀察BV-2細(xì)胞活化反應(yīng)。然后將被激活的BV-2細(xì)胞培養(yǎng)液上清加入661w細(xì)胞中,觀察661w的凋亡情況�；�?qū)ⅵ纶吇蜃又苯蛹尤氲?61w細(xì)胞中,觀察其凋亡情況。事先加入β趨化因子抑制劑met-RANTES后,重復(fù)上述操作。主要指標(biāo)鈣內(nèi)流、ROS及NO的產(chǎn)生、TUNEL陽(yáng)性感光細(xì)胞百分比。結(jié)果β趨化因子加入BV-2細(xì)胞后,細(xì)胞內(nèi)鈣流曲線明顯上升、細(xì)胞外ROS及NO產(chǎn)生較對(duì)照組均明顯增高(P0.01);將激活后的BV-2細(xì)胞上清加入到661w細(xì)胞中,后者凋亡率增加30%。β趨化因子直接加入661w細(xì)胞中,上述指標(biāo)無(wú)明顯變化。在加入β趨化因子之前先加入其抑制劑met-RANTES,BV-2細(xì)胞的ROS產(chǎn)生較未加抑制劑組明顯降低(P0.01)、NO產(chǎn)生較未加抑制劑組降低(P=0.0187),661w細(xì)胞凋亡明顯減少。結(jié)論β趨化因子可活化小鼠小膠質(zhì)細(xì)胞導(dǎo)致感光細(xì)胞凋亡。β趨化因子抑制劑可抑制遺傳性視網(wǎng)膜變性小鼠小膠質(zhì)細(xì)胞活化,保護(hù)感光細(xì)胞。
[Abstract]:Objective to observe the effect of 尾 chemokine on the activation of mouse microglia and the induction of photoreceptor cell apoptosis. Design experimental studies. Object 尾 chemokine, mouse BV-2 microglia cell line and 661w photoreceptor cell line were studied. Methods 尾 chemokines (RANTES,MIP-1 偽 and MIP-1 尾) were added to BV-2 cells to observe the activation of BV-2 cells. Then the supernatant of activated BV-2 cells was added to 661w cells to observe the apoptosis at 661w. Or 尾 chemokine was added directly to 661w cells to observe its apoptosis. After adding 尾 chemokine inhibitor met-RANTES in advance, repeat the above operation. Main outcome measures calcium influx, production of ROS and NO, percentage of TUNEL positive photosensitive cells. Results after the addition of 尾 chemokine to BV-2 cells, the intracellular calcium flow curve increased significantly, and the production of extracellular ROS and NO increased significantly compared with the control group (P0.01). When the activated supernatant of BV-2 cells was added to 661w cells, the apoptotic rate of the latter increased by 30%, and 尾 chemokine was added directly into 661w cells. The ROS production of met-RANTES,BV-2 cells with 尾 chemokine was significantly lower than that of non-inhibitor group (P0.01), NO production was lower than that of non-inhibitor group (P0. 0187), and apoptosis was significantly decreased at 661w. Conclusion 尾 chemokine can activate mouse microglia and induce photoreceptor cell apoptosis. 尾 chemokine inhibitor can inhibit the activation of microglia and protect photoreceptor cells in mice with hereditary retinal degeneration.
【作者單位】： 首都醫(yī)科大學(xué)附屬北京同仁醫(yī)院北京同仁眼科中心北京市眼科研究所;眼科學(xué)與視覺(jué)科學(xué)北京市重點(diǎn)實(shí)驗(yàn)室;中國(guó)科學(xué)院北京基因研究所;
【基金】：國(guó)家自然科學(xué)基金(81100675) 北京市自然科學(xué)基金(7102034)
【分類號(hào)】：R774.13
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本文編號(hào)：2367374