Nogo-A，NgR在大鼠視網(wǎng)膜缺血再灌注損傷中的表達
發(fā)布時間：2018-10-30 19:51
【摘要】：目的觀察軸突生長抑制因子Nogo-A及其受體NgR在視網(wǎng)膜缺血再灌注(Retinal ischemia-reperfusion,RIR)急性損傷中的表達,研究兩者在RIR損傷中的作用及相關(guān)性。方法90只SD大鼠隨機分為：正常對照組(n=6只)；假手術(shù)組(n=42只)；RIR組(n=42只),假手術(shù)組及RIR組分為再灌注后0、6、12、24、48、72、168h亞組,每組6只；采用結(jié)扎單側(cè)頸總動脈的方法制備大鼠RIR模型,HE染色觀察組織形態(tài)學(xué)改變,免疫組織化學(xué)檢測Nogo-A和NgR蛋白的表達。結(jié)果假手術(shù)組各時間點Nogo-A及NgR表達與正常組相比無差異(P0.05),實驗組大鼠與假手術(shù)組相比：Nogo-A的表達在12h開始升高(P0.05),48h達到高峰(P0.01),72h下降(P0.05),168h達到正�；�水平(P0.05)；NgR的表達在6h即出現(xiàn)上升(P0.05),持續(xù)至48h達到最高峰(P0.01),72h下降(P0.05),168h達到正�；�水平(P0.05)。實驗組大鼠Nogo-A與NgR的表達呈正相關(guān)(P0.01)。結(jié)論Nogo-A及NgR在RIR各時間點均有表達,其表達水平沿時間點呈拋物線形,在再灌注48h時均達到峰值,NgR先于Nogo-A表達,兩者協(xié)同作用,與RIR損傷后抑制節(jié)細胞軸突修復(fù)再生相關(guān)。
[Abstract]:Objective to investigate the expression of axon growth inhibitor (Nogo-A) and its receptor NgR in acute retinal ischemia-reperfusion (Retinal ischemia-reperfusion,RIR) injury. Methods 90 SD rats were randomly divided into normal control group (n = 6), sham operation group (n = 42); RIR group (n = 42), sham operation group (n = 42) and RIR group (n = 6). The rat RIR model was established by ligating unilateral common carotid artery. The histomorphologic changes were observed by HE staining and the expression of Nogo-A and NgR proteins were detected by immunohistochemistry. Results there was no difference in the expression of Nogo-A and NgR between the sham operation group and the normal group (P0.05). Compared with the sham operation group, the expression of Nogo-A in the experimental group began to increase at 12 h (P0.05), and reached the peak at 48 h (P0.01). 72h decreased (P0.05), 168h reached the normal baseline level (P0.05); The expression of NgR increased at 6h (P0.05), reached its peak at 48h (P0.01), decreased at 72h (P0.05), and reached the normal baseline level at 168h (P0.05). There was a positive correlation between Nogo-A and NgR expression in experimental group (P0.01). Conclusion Nogo-A and NgR were expressed at each time point of RIR, and the expression level was parabola along the time point and reached its peak value at 48h after reperfusion. The expression of NgR was earlier than that of Nogo-A. It is related to the inhibition of axon repair and regeneration of ganglion cells after RIR injury.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R774.1
[Abstract]:Objective to investigate the expression of axon growth inhibitor (Nogo-A) and its receptor NgR in acute retinal ischemia-reperfusion (Retinal ischemia-reperfusion,RIR) injury. Methods 90 SD rats were randomly divided into normal control group (n = 6), sham operation group (n = 42); RIR group (n = 42), sham operation group (n = 42) and RIR group (n = 6). The rat RIR model was established by ligating unilateral common carotid artery. The histomorphologic changes were observed by HE staining and the expression of Nogo-A and NgR proteins were detected by immunohistochemistry. Results there was no difference in the expression of Nogo-A and NgR between the sham operation group and the normal group (P0.05). Compared with the sham operation group, the expression of Nogo-A in the experimental group began to increase at 12 h (P0.05), and reached the peak at 48 h (P0.01). 72h decreased (P0.05), 168h reached the normal baseline level (P0.05); The expression of NgR increased at 6h (P0.05), reached its peak at 48h (P0.01), decreased at 72h (P0.05), and reached the normal baseline level at 168h (P0.05). There was a positive correlation between Nogo-A and NgR expression in experimental group (P0.01). Conclusion Nogo-A and NgR were expressed at each time point of RIR, and the expression level was parabola along the time point and reached its peak value at 48h after reperfusion. The expression of NgR was earlier than that of Nogo-A. It is related to the inhibition of axon repair and regeneration of ganglion cells after RIR injury.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R774.1
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本文編號：2301000
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