發(fā)布時(shí)間：2018-09-12 10:40

【摘要】：背景:有研究發(fā)現(xiàn)硫酸軟骨素酶降解硫酸軟骨素蛋白多糖能夠促使視網(wǎng)膜上Müller細(xì)胞的移行,但硫酸軟骨素酶降解硫酸軟骨素蛋白多糖是否能促進(jìn)脂肪間充質(zhì)干細(xì)胞在視網(wǎng)膜變性大鼠視網(wǎng)膜的移行尚不明確。目的:探討硫酸軟骨素酶降解硫酸軟骨素蛋白多糖對(duì)脂肪間充質(zhì)干細(xì)胞治療大鼠視網(wǎng)膜變性的影響。方法:分離并培養(yǎng)人脂肪間充質(zhì)干細(xì)胞,建立視網(wǎng)膜變性大鼠模型,向視網(wǎng)膜變性大鼠視網(wǎng)膜下腔注射脂肪間充質(zhì)干細(xì)胞+硫酸軟骨素酶,觀察移植后大鼠脂肪間充質(zhì)干細(xì)胞遷移率和視網(wǎng)膜細(xì)胞凋亡情況。結(jié)果與結(jié)論:人脂肪間充質(zhì)干細(xì)胞能夠成功培養(yǎng),Brd U對(duì)人脂肪間充質(zhì)干細(xì)胞的標(biāo)記率達(dá)90.0%以上。建模后7 d,視網(wǎng)膜外核層塌陷,光感受器細(xì)胞大量外節(jié)迸解,外核層貼附在Bruch’s膜上,視網(wǎng)膜呈拱橋樣,中央視網(wǎng)膜和外周視網(wǎng)膜均受到損傷;正常大鼠視網(wǎng)膜各層清晰,光感受器細(xì)胞排列規(guī)律,視網(wǎng)膜色素上皮層完整。脂肪間充質(zhì)干細(xì)胞+硫酸軟骨素酶組脂肪間充質(zhì)干細(xì)胞遷移率高于脂肪間充質(zhì)干細(xì)胞組,且2組視網(wǎng)膜細(xì)胞凋亡率比較差異無(wú)顯著性意義。表明硫酸軟骨素酶降解硫酸軟骨素蛋白多糖可提高人脂肪間充質(zhì)干細(xì)胞在視網(wǎng)膜上的遷移能力。
[Abstract]:Background: chondroitin sulfate enzyme degradation of chondroitin sulfate proteoglycan has been found to promote the migration of M 眉 ller cells in the retina. However, whether chondroitin sulfate enzyme degradation of chondroitin sulfate proteoglycan can promote the migration of adipose mesenchymal stem cells in retina of rats with retinal degeneration is not clear. Aim: to investigate the effect of chondroitin sulfate enzyme degradation of chondroitin sulfate proteoglycan on retinal degeneration in rats treated with adipose mesenchymal stem cells. Methods: human adipose mesenchymal stem cells were isolated and cultured, and the rat model of retinal degeneration was established. Chondroitin sulfate from adipose mesenchymal stem cells was injected into the subretinal cavity of rats with retinal degeneration. The migration rate of adipose mesenchymal stem cells and apoptosis of retinal cells were observed after transplantation. Results & conclusion: human adipose mesenchymal stem cells could be successfully cultured with BrdU to label human adipose mesenchymal stem cells with a rate of more than 90.0%. 7 days after modeling, the outer nuclear layer of the retina collapsed and a large number of outer segments of the photoreceptor cells burst out. The outer nuclear layer attached to the Bruch's membrane showed an arch bridge of the retina, the central retina and the peripheral retina were damaged, and all layers of the normal rat retina were clear. The photoreceptor cells were arranged regularly and the retinal pigment epithelium was intact. The migration rate of adipose mesenchymal stem cells in chondroitin sulfate group was higher than that in adipose mesenchymal stem cells group. The results showed that chondroitin sulfate enzyme degradation of chondroitin sulfate proteoglycan could improve the migration of human adipose mesenchymal stem cells on the retina.
【作者單位】： 河北醫(yī)科大學(xué)第一醫(yī)院眼科;
【基金】：河北省醫(yī)學(xué)科學(xué)研究課題計(jì)劃項(xiàng)目(20130589)~~
【分類號(hào)】：R774.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 李偉;王翠U，

本文編號(hào)：2238748