【摘要】：鞏膜膠原交聯(lián)術(shù)是有望控制近視病程發(fā)展的方法之一。然而,該術(shù)式的確切程序及其對(duì)近視動(dòng)物模型的影響仍有待探討。將離子導(dǎo)入法引入鞏膜核黃素-紫外線A(UVA)交聯(lián)術(shù),采用10mW/cm~2,9min的快速交聯(lián)方案,研究該術(shù)式對(duì)于近視動(dòng)物模型鞏膜生物力學(xué)性能及膠原代謝的影響。利用形覺(jué)剝奪法將96只新西蘭大白兔誘導(dǎo)形成相對(duì)穩(wěn)定的近視模型,然后進(jìn)行鞏膜交聯(lián)術(shù)或假手術(shù),術(shù)后4個(gè)時(shí)間點(diǎn)(1d,10d,1個(gè)月,3個(gè)月)分別采集樣本和獲取數(shù)據(jù)。術(shù)后4個(gè)時(shí)間點(diǎn),與非交聯(lián)近視眼相比,交聯(lián)近視眼鞏膜的極限應(yīng)力、楊氏模量和生理?xiàng)钍夏Ａ烤黠@增加。此外,交聯(lián)組與未交聯(lián)組膠原代謝相關(guān)基因和蛋白的表達(dá)存在顯著的差異。結(jié)果表明,離子導(dǎo)入核黃素-紫外線A快速交聯(lián)術(shù)很可能是一種控制近視病理過(guò)程的可行性方法。
[Abstract]:Scleral collagen crosslinking is one of the promising methods to control the progression of myopia. However, the exact procedure of this procedure and its effect on the animal model of myopia need to be discussed. The scleral riboflavin ultraviolet A (UVA) crosslinking was introduced into the scleral sclera. The effects of this procedure on the scleral biomechanical properties and collagen metabolism in myopic animal models were studied by a rapid crosslinking protocol of 10 MW / cm ~ 2 for 9 min. Ninety-six New Zealand white rabbits were induced to form a relatively stable myopia model by the method of form deprivation. Scleral crosslinking or sham-operation was performed. Samples were collected and data were obtained at 4 time points (1 day, 10 days, 1 month, 3 months) after operation. The scleral ultimate stress, Young's modulus and physiological Young's modulus of cross-linked myopia were significantly increased compared with non-cross-linked myopia at 4 time points after operation. In addition, there were significant differences in the expression of collagen metabolism-related genes and proteins between crosslinked and uncrosslinked groups. The results showed that the rapid cross-linking of riboflavin and ultraviolet A might be a feasible method to control the pathological process of myopia.
【作者單位】： 太原理工大學(xué)應(yīng)用力學(xué)與生物醫(yī)學(xué)工程研究所;白求恩國(guó)際和平醫(yī)院眼科;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(31271005,11402162)
【分類號(hào)】：R779.6

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