【摘要】：目的檢測(cè)半胱天冬酶12(caspase-12)在糖尿病(DM)大鼠視網(wǎng)膜的表達(dá)情況,探討內(nèi)質(zhì)網(wǎng)應(yīng)激在糖尿病視網(wǎng)膜病變(DR)中的作用。方法健康雄性SD大鼠30只隨機(jī)分成空白組(CON組)15只,糖尿病1月組(DM1組)、糖尿病2月組(DM2組)、糖尿病3月組(DM3組),每組5只。分別于造模后1,2,3個(gè)月摘除眼球,行HE染色、免疫組織化學(xué)染色,觀察視網(wǎng)膜形態(tài),計(jì)數(shù)視網(wǎng)膜神經(jīng)節(jié)細(xì)胞(RCGs),并檢測(cè)caspase-12在視網(wǎng)膜的表達(dá)情況。結(jié)果視網(wǎng)膜HE染色顯示DM組大鼠視網(wǎng)膜神經(jīng)節(jié)細(xì)胞減少,內(nèi)外核層細(xì)胞稀疏,排列紊亂,網(wǎng)狀層空泡樣變性等改變,且隨病程延長(zhǎng)逐漸加重。RCGs計(jì)數(shù)結(jié)果 CON組、DM1組、DM2組、DM3組結(jié)果分別是281.20±7.98,263.60±13.37,240.60±11.78,194.80±13.16,各組組間差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。免疫組織化學(xué)染色顯示,caspase-12在CON組沒(méi)有陽(yáng)性表達(dá),在DM組主要表達(dá)于神經(jīng)節(jié)細(xì)胞層。隨著病程進(jìn)展,內(nèi)核層也有表達(dá),且表達(dá)強(qiáng)度逐漸增強(qiáng)。DM1組、DM2組、DM3組caspase-12表達(dá)的平均光密度值(IOD/area)分別是0.0173±0.0039,0.0348±0.0050,0.0579±0.0074,DM2組和DM3組與DM1組比較差異有統(tǒng)計(jì)學(xué)意義(P0.01)。結(jié)論隨著DM病程進(jìn)展,DR大鼠RGCs凋亡逐漸增加,caspase-12誘導(dǎo)的內(nèi)質(zhì)網(wǎng)凋亡在DR發(fā)病中有重要作用,內(nèi)質(zhì)網(wǎng)應(yīng)激促進(jìn)了DR的發(fā)生、發(fā)展。
[Abstract]:Objective to detect the expression of cysteinase 12 (caspase-12) in the retina of diabetic (DM) rats and to explore the role of endoplasmic reticulum stress in diabetic retinopathy (DR). Methods Thirty healthy male Sprague-Dawley rats were randomly divided into control group (CON group, n = 15), diabetic group (DM1 group, n = 15), diabetic group (DM2 group, n = 5) and diabetic group (DM3 group, n = 5). The eyeballs were removed at 1 ~ 2 and 3 months after the model. The retinal morphology was observed by HE staining and immunohistochemical staining. The (RCGs), of retinal ganglion cells was counted and the expression of caspase-12 in the retina was detected. Results Retinal HE staining showed that the retinal ganglion cells were decreased, the cells in the inner and outer nuclear layers were sparse, the arrangement was disordered, and the vacuolar degeneration of the reticular layer was changed in DM group. The results of RCGs count in CON group were 281.20 鹵7.98263.60 鹵13.37240.60 鹵11.78194.80 鹵13.16, respectively (P0.05). Immunohistochemical staining showed that caspase-12 was not expressed in CON group, but mainly in ganglion cell layer in DM group. With the progression of the disease, the expression of caspase-12 was also found in the kernel layer, and the average optical density (IOD/area) of caspase-12 expression in DM2 group was 0.0173 鹵0.0039 鹵0.0050 鹵0.054 鹵0.0579 鹵0.0074 in DM2 group and DM3 group was significantly higher than that in DM1 group (P0.01). Conclusion the apoptosis of endoplasmic reticulum induced by caspase-12 plays an important role in the pathogenesis of Dr with the progression of DM. Endoplasmic reticulum stress promotes the occurrence and development of Dr.
【作者單位】： 濰坊醫(yī)學(xué)院眼科學(xué)教研室;濰坊醫(yī)學(xué)院附屬醫(yī)院眼科;
【基金】：山東省教育廳資助課題(課題編號(hào):J10LF6)
【分類號(hào)】：R587.1;R774.1
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本文編號(hào)：2182860