大鼠外側(cè)膝狀體神經(jīng)元時空感受野特性研究
發(fā)布時間：2018-08-04 18:28
【摘要】：目的 觀察大鼠外側(cè)膝狀體(LGN)神經(jīng)元感受野時空頻率調(diào)諧特性的發(fā)育變化以及異常視覺經(jīng)驗(yàn)(單眼形覺剝奪)對LGN神經(jīng)元時空頻率調(diào)諧及局部震蕩特性的影響。 方法 1.睜眼后14-16d組、睜眼后20-22d組、睜眼后27-30d組、睜眼后60d組Wistar大鼠各5只,用在體細(xì)胞外記錄技術(shù),正弦光柵視覺刺激檢測各組大鼠LGN神經(jīng)元感受野時空調(diào)諧特性,觀察大鼠LGN神經(jīng)元時空頻率調(diào)諧特性發(fā)育之動態(tài)變化。 2.單眼形覺剝奪Wistar大鼠10只,用在體細(xì)胞外記錄技術(shù),正弦光柵視覺刺激,觀察LGN剝奪層與非剝奪層神經(jīng)元感受野時空頻率調(diào)諧特性及局部震蕩特性的變化。 結(jié)果 1.睜眼后14-16d組、睜眼后20-22d組、睜眼后27-30d組、睜眼后60d組大鼠LGN神經(jīng)元時間和空間頻率調(diào)諧低通和帶通間的分布差異均無統(tǒng)計(jì)學(xué)意義(χ2＝0.68,0.47, P0.05),4個組大鼠LGN神經(jīng)元感受野最優(yōu)時間頻率調(diào)諧特性成年后達(dá)最高值,最優(yōu)時間頻率均值分別為(2.6±1.5)、(2.6±1.5)、(2.5±1.5)、(3.6±2.3) cycles/s,差異有統(tǒng)計(jì)學(xué)意義(F=3.4,P0.05),睜眼后60d組的最優(yōu)時間頻率明顯高于睜眼后14-16d組、睜眼后20～22d組、睜眼后27-30d組,差異有統(tǒng)計(jì)學(xué)意義(q=4.43,4.10,4.03,P0.05),余3組間的相互比較差異均無統(tǒng)計(jì)學(xué)意義(P0.05)。四組瞬時帶寬均值在1.7±1.9octaves,各年齡組之間比較,差異無統(tǒng)計(jì)學(xué)意義(F=0.22,P0.05)。4組大鼠LGN神經(jīng)元的最優(yōu)空間頻率(中值)分別為0.03、0.03、0.035、0.04cycles/deg,空間分辨率為(0.26士0.15)、(0.26±0.15)、(0.28±0.13)、(0.29±0.14) cycles/deg,空間帶寬分別(2.7士1.2)、(2.8±1.2)、(3.0±1.0)、(2.4±1.0)octaves,上述三項(xiàng)指標(biāo)差異均無統(tǒng)計(jì)學(xué)意義(F=0.34、1.23、0.50,P0.05)。 方向敏感性細(xì)胞、方位敏感性細(xì)胞及無方向選擇性細(xì)胞比例各年齡組分布大致相同：方向選擇性細(xì)胞約占五分之一,方位選擇性細(xì)胞約占三分之二,余為方向選擇性不敏感細(xì)胞(x2=0.26,P0.05)。隨著年齡的增長,LGN神經(jīng)元對比度閾值逐漸降低,成年前三組分別為(31.5±17.8)%、(29.1±16.6)%、(28.2±18.4)%,到成年60d時達(dá)到最低(19.4±17.5)%,較成年前3組間比較差別具有統(tǒng)計(jì)學(xué)意義(F＝3.2,P0.05,q=11.98.8.30.7.90),成年前3組間差別無統(tǒng)計(jì)學(xué)意義(P0.05)。 2.LGN神經(jīng)元時空間頻率調(diào)制細(xì)胞數(shù)與非調(diào)制細(xì)胞數(shù)各組之間差別弱剝奪組與非剝奪組之間比較差異無統(tǒng)計(jì)學(xué)意義(χ2=0.00,P0.05)。剝奪層神經(jīng)元感受野的最優(yōu)時間頻率為(2.5±1.4)cycles/s,非剝奪層支配LGN細(xì)胞最優(yōu)時間頻率為(2.5±1.3)cycles/s,剝奪組與非剝奪組差別無統(tǒng)計(jì)學(xué)意義(t=0.013,P0.05)；剝奪層局部場電位(LFP) γ-波段(25-90赫茲)功率譜為(11.65±4.72)V^2*e-009,非剝奪層γ-波段功率譜為(14.09±3.90) V^2*e-009,剝奪組明顯低于非剝奪組,差別具有統(tǒng)計(jì)學(xué)意義((t=2.93,P0.05)。 剝奪層神經(jīng)元感受野最優(yōu)空間頻率為0.03(中值)cycles/deg,空間分辨率為(0.26±0.14)cycles/deg,非剝奪層最優(yōu)空間頻率0.03(中值)cycles/deg,空間分辨率為(0.27±0.13)cycles/deg,最優(yōu)空間頻率、空間分辨率二者之間差別均無統(tǒng)計(jì)意義(χ2=0.34,t＝1.23,P0.05)；剝奪層LFP Y-波段功率譜為(16.73±7.34)V^2*e-009,非剝奪層γ-波段功率譜為(7.27±3.0)V^2*e-009,剝奪組明顯低于非剝奪組,差別具有統(tǒng)計(jì)學(xué)意義(t=8.5,P0.05)。 結(jié)論 1.睜眼后14-14d、睜眼后20-22d、睜眼后27～30d、睜眼后60d大鼠LGN神經(jīng)元感受野空間頻率、方位和方向選擇性調(diào)諧特性在睜眼后2天即已發(fā)育成熟。時間頻率、對比度,至成年才成熟。時空頻率調(diào)諧發(fā)育特性的不同可能與其在視覺通路中的功能有關(guān)。 2.單眼形覺剝奪不改變大鼠LGN神經(jīng)元時空感受野特性,但局部神經(jīng)元集群處理時空信息的活動明顯減弱,單眼形覺剝奪影響大鼠LGN神經(jīng)元集群活動,為研究弱視發(fā)生機(jī)制提供了一個新的線索。
[Abstract]:objective
The changes in the spatial and temporal tuning characteristics of the neurons of the lateral geniculate (LGN) neurons in the rat lateral geniculate body and the effects of abnormal visual experience (monocular form deprivation) on the temporal and spatial frequency tuning and local oscillation of LGN neurons were observed.
Method
1. after opening eyes, group 14-16d, group 20-22d after opening eyes, group 27-30d after opening eyes, 5 Wistar rats in group 60d after opening eyes, using the technique of extracellular recording, and sinusoidal grating visual stimulation to detect the temporal and spatial tuning characteristics of LGN neurons in the rats of each group, and observe the dynamic changes of the time and space frequency tuning characteristics of LGN neurons in the rat LGN.
2. the 10 Wistar rats with monocular form deprivation were used in the extracellular recording technique and sinusoidal grating visual stimulation to observe the frequency tuning and local oscillation characteristics of the LGN deprivation and non deprived neurons in the receptive field.
Result
1. after open eyes, group 14-16d, group 20-22d after opening eyes, group 27-30d after opening eyes, LGN neurons in group 60d after opening eyes, LGN neuron time and space frequency tuning low pass and the distribution difference between the band is not statistically significant (x 2 = 0.68,0.47, P0.05), the optimal time frequency tuning characteristic of the 4 groups of rat LGN neurons is the highest, the optimal time frequency is the highest, the optimal time frequency The average rate was (2.6 + 1.5), (2.6 + 1.5), (2.5 + 1.5), (3.6 + 2.3) cycles/s, and the difference was statistically significant (F=3.4, P0.05). The optimal time frequency of group 60d was significantly higher than that of group 14-16d after open eyes, and group 20 to 22d after opening eyes, and the difference was statistically significant (q=4.43,4.10,4.03, P0.05) after opening eyes (q=4.43,4.10,4.03, P0.05), and the differences of the remaining 3 groups were all different There was no statistical significance (P0.05). The mean instantaneous bandwidth in the four group was 1.7 + 1.9octaves, and the difference was not statistically significant (F=0.22, P0.05), the optimal spatial frequency (median) of LGN neurons in the.4 group was 0.03,0.03,0.035,0.04cycles/deg, the spatial differentiation rate was (0.26 0.15), (0.26 + 0.15), (0.28 + 0.13), (0.29 + 0.14) Cycl ES / deg, spatial bandwidth were (2.7s 1.2), (2.8 + 1.2), (3.0 + 1.0), (2.4 + 1.0) octaves, respectively. There was no significant difference among the three indexes (F = 0.34, 1.23, 0.50, P 0.05).
The proportion of direction sensitive cells, azimuth sensitive cells and non directional selective cells was approximately the same in all age groups: the direction selective cells accounted for about 1/5, azimuth selective cells accounted for about 2/3, and the other was directional selective insensitive cells (x2=0.26, P0.05). As the age increased, the contrast threshold of LGN neurons decreased gradually. The three groups in the first three groups were (31.5 + 17.8)%, (29.1 + 16.6)% and (28.2 + 18.4)%, reaching the lowest (19.4 + 17.5)% to adult 60d. The difference was statistically significant compared with the 3 groups (F = 3.2, P0.05, q=11.98.8.30.7.90), and there was no statistically significant difference between the three groups before adulthood (P0.05).
The difference between the number of spatial frequency modulation cells and the number of non modulation cells in 2.LGN neurons was not statistically significant (x 2=0.00, P0.05). The optimal time frequency of the deprivation neurons was (2.5 + 1.4) cycles/s, and the optimal time frequency of the non deprived LGN cells was (2.5 + 1.3) cycles/s. There was no significant difference between the deprivation group and the non deprived group (t=0.013, P0.05); the power spectrum of the partial field potential (LFP) gamma band (25-90 Hz) of the deprivation layer was (11.65 + 4.72) V^2*e-009, the non deprivation layer gamma band power spectrum was (14.09 + 3.90) V^2*e-009, and the deprivation group was significantly lower than that of the non deprived group (t=2.93, P0.05).
The optimal spatial frequency of the deprivation neurons was 0.03 (median) cycles/deg, the spatial resolution was (0.26 + 0.14) cycles/deg, the optimal spatial frequency of the non deprivation layer was 0.03 (median) cycles/deg, the spatial resolution was (0.27 + 0.13) cycles/deg, the optimal spatial frequency and the space resolution two had no statistical significance (x 2=0.34, t = 1.23, P0.0). 5) the power spectrum of the LFP Y- band of the deprivation layer was (16.73 + 7.34) V^2*e-009, the non deprivation layer gamma band power spectrum was (7.27 + 3) V^2*e-009, and the deprivation group was significantly lower than that of the non deprived group. The difference was statistically significant (t=8.5, P0.05).
conclusion
1. after opening eyes, 14-14d, 20-22d after opening eyes, 27 to 30d after opening eyes, and after opening eyes, LGN neurons of 60d rats feel the spatial frequency of field. The selective tuning characteristics of azimuth and direction are mature at 2 days after opening eyes. Time frequency, contrast, mature. The difference of temporal and spatial frequency tuning developmental characteristics may be related to its function in the visual pathway Close.
2. monocular form deprivation does not change the space-time receptive field characteristics of LGN neurons in rats, but the activity of the local neurons in the processing of spatio-temporal information is obviously weakened. Monocular form deprivation affects the activity of LGN neurons in rats, and provides a new clue for the study of the mechanism of amblyopia.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2012
【分類號】：R777.44
本文編號：2164732
[Abstract]:objective
The changes in the spatial and temporal tuning characteristics of the neurons of the lateral geniculate (LGN) neurons in the rat lateral geniculate body and the effects of abnormal visual experience (monocular form deprivation) on the temporal and spatial frequency tuning and local oscillation of LGN neurons were observed.
Method
1. after opening eyes, group 14-16d, group 20-22d after opening eyes, group 27-30d after opening eyes, 5 Wistar rats in group 60d after opening eyes, using the technique of extracellular recording, and sinusoidal grating visual stimulation to detect the temporal and spatial tuning characteristics of LGN neurons in the rats of each group, and observe the dynamic changes of the time and space frequency tuning characteristics of LGN neurons in the rat LGN.
2. the 10 Wistar rats with monocular form deprivation were used in the extracellular recording technique and sinusoidal grating visual stimulation to observe the frequency tuning and local oscillation characteristics of the LGN deprivation and non deprived neurons in the receptive field.
Result
1. after open eyes, group 14-16d, group 20-22d after opening eyes, group 27-30d after opening eyes, LGN neurons in group 60d after opening eyes, LGN neuron time and space frequency tuning low pass and the distribution difference between the band is not statistically significant (x 2 = 0.68,0.47, P0.05), the optimal time frequency tuning characteristic of the 4 groups of rat LGN neurons is the highest, the optimal time frequency is the highest, the optimal time frequency The average rate was (2.6 + 1.5), (2.6 + 1.5), (2.5 + 1.5), (3.6 + 2.3) cycles/s, and the difference was statistically significant (F=3.4, P0.05). The optimal time frequency of group 60d was significantly higher than that of group 14-16d after open eyes, and group 20 to 22d after opening eyes, and the difference was statistically significant (q=4.43,4.10,4.03, P0.05) after opening eyes (q=4.43,4.10,4.03, P0.05), and the differences of the remaining 3 groups were all different There was no statistical significance (P0.05). The mean instantaneous bandwidth in the four group was 1.7 + 1.9octaves, and the difference was not statistically significant (F=0.22, P0.05), the optimal spatial frequency (median) of LGN neurons in the.4 group was 0.03,0.03,0.035,0.04cycles/deg, the spatial differentiation rate was (0.26 0.15), (0.26 + 0.15), (0.28 + 0.13), (0.29 + 0.14) Cycl ES / deg, spatial bandwidth were (2.7s 1.2), (2.8 + 1.2), (3.0 + 1.0), (2.4 + 1.0) octaves, respectively. There was no significant difference among the three indexes (F = 0.34, 1.23, 0.50, P 0.05).
The proportion of direction sensitive cells, azimuth sensitive cells and non directional selective cells was approximately the same in all age groups: the direction selective cells accounted for about 1/5, azimuth selective cells accounted for about 2/3, and the other was directional selective insensitive cells (x2=0.26, P0.05). As the age increased, the contrast threshold of LGN neurons decreased gradually. The three groups in the first three groups were (31.5 + 17.8)%, (29.1 + 16.6)% and (28.2 + 18.4)%, reaching the lowest (19.4 + 17.5)% to adult 60d. The difference was statistically significant compared with the 3 groups (F = 3.2, P0.05, q=11.98.8.30.7.90), and there was no statistically significant difference between the three groups before adulthood (P0.05).
The difference between the number of spatial frequency modulation cells and the number of non modulation cells in 2.LGN neurons was not statistically significant (x 2=0.00, P0.05). The optimal time frequency of the deprivation neurons was (2.5 + 1.4) cycles/s, and the optimal time frequency of the non deprived LGN cells was (2.5 + 1.3) cycles/s. There was no significant difference between the deprivation group and the non deprived group (t=0.013, P0.05); the power spectrum of the partial field potential (LFP) gamma band (25-90 Hz) of the deprivation layer was (11.65 + 4.72) V^2*e-009, the non deprivation layer gamma band power spectrum was (14.09 + 3.90) V^2*e-009, and the deprivation group was significantly lower than that of the non deprived group (t=2.93, P0.05).
The optimal spatial frequency of the deprivation neurons was 0.03 (median) cycles/deg, the spatial resolution was (0.26 + 0.14) cycles/deg, the optimal spatial frequency of the non deprivation layer was 0.03 (median) cycles/deg, the spatial resolution was (0.27 + 0.13) cycles/deg, the optimal spatial frequency and the space resolution two had no statistical significance (x 2=0.34, t = 1.23, P0.0). 5) the power spectrum of the LFP Y- band of the deprivation layer was (16.73 + 7.34) V^2*e-009, the non deprivation layer gamma band power spectrum was (7.27 + 3) V^2*e-009, and the deprivation group was significantly lower than that of the non deprived group. The difference was statistically significant (t=8.5, P0.05).
conclusion
1. after opening eyes, 14-14d, 20-22d after opening eyes, 27 to 30d after opening eyes, and after opening eyes, LGN neurons of 60d rats feel the spatial frequency of field. The selective tuning characteristics of azimuth and direction are mature at 2 days after opening eyes. Time frequency, contrast, mature. The difference of temporal and spatial frequency tuning developmental characteristics may be related to its function in the visual pathway Close.
2. monocular form deprivation does not change the space-time receptive field characteristics of LGN neurons in rats, but the activity of the local neurons in the processing of spatio-temporal information is obviously weakened. Monocular form deprivation affects the activity of LGN neurons in rats, and provides a new clue for the study of the mechanism of amblyopia.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2012
【分類號】：R777.44
【參考文獻(xiàn)】
相關(guān)期刊論文 前1條
1 ;Effects of age on latency and variability of visual response in monkeys[J];Chinese Science Bulletin;2005年11期
，本文編號：2164732
本文鏈接：http://sikaile.net/yixuelunwen/wuguanyixuelunwen/2164732.html
最近更新
教材專著
