microRNA-126在多能干細胞定向分化為視網(wǎng)膜神經干細胞過程中表達量的變化及意義
發(fā)布時間：2018-08-01 16:12
【摘要】：目的研究microRNA-126(miR-126)在濕性老年黃斑變性(wet AMD)疾病患者來源的多能干細胞(i PSC)定向分化為視網(wǎng)膜神經干細胞(RNSC)中表達量的變化及意義。方法選取新發(fā)現(xiàn)的wet AMD患者10例作為觀察組,無眼底病變及全身病的10例作為對照組。抽取受試者的外周靜脈血,提取單個核細胞(PBMC),進一步提取CD34+細胞,行腺病毒轉染,誘導產生i PSC且進行鑒定;應用實時定量PCR(qRT-PCR)技術檢測miR-126在10個不同誘導發(fā)育階段的RNSC中的相對表達量。結果 wet AMD疾病患者的外周血成功誘導分化出RNSC;觀察組miR-126的不同誘導時間相對表達量的差異除了時刻8、9、10其余測試時間點均有統(tǒng)計學意義(P0.05);對照組miR-126的不同誘導時間相對表達量的差異無統(tǒng)計學意義(P0.05);觀察組與對照組細胞miR-126相對表達量在不同誘導時間的差異有統(tǒng)計學意義(P0.05)。結論 wet AMD患者早期miR-126的表達量顯著升高,且有逐漸降低的趨勢。
[Abstract]:Objective to study the changes and significance of microRNA-126 (miR-126) expression in the differentiation of multipotent stem cells (i PSC) into retinal neural stem cells (RNSC) in elderly patients with (wet AMD) disease of macular degeneration. Methods Ten newly discovered wet AMD patients were selected as observation group, 10 patients without fundus disease and 10 patients with systemic disease as control group. The peripheral venous blood was extracted and the (PBMC), of mononuclear cells was extracted. The CD34 cells were further extracted by adenovirus transfection and I PSC was induced and identified. Real time quantitative PCR (qRT-PCR) was used to detect the relative expression of miR-126 in 10 RNSC at different stages of induction and development. Results RNSCs were successfully induced and differentiated from peripheral blood of patients with wet AMD disease, and the difference of relative expression of miR-126 at different induction time in observation group was statistically significant (P0.05), and that in control group was different in different miR-126 induction time (P0.05). There was no significant difference in the relative expression of miR-126 between the two groups (P0.05); there was significant difference in the relative expression of miR-126 between the observation group and the control group at different induction time (P0.05). Conclusion the expression of miR-126 in the early stage of wet AMD patients increased significantly and tended to decrease gradually.
【作者單位】： 深圳市眼科醫(yī)院暨南大學醫(yī)學院深圳眼科中心深圳眼科學重點實驗室;
【基金】：深圳市科技創(chuàng)新委員會國際合作項目(編號:GJHZ20150316150106271) 深圳市“三名工程”項目 廣東省中醫(yī)藥局科研項目(編號:20171239)
【分類號】：R774.5
本文編號：2158121
[Abstract]:Objective to study the changes and significance of microRNA-126 (miR-126) expression in the differentiation of multipotent stem cells (i PSC) into retinal neural stem cells (RNSC) in elderly patients with (wet AMD) disease of macular degeneration. Methods Ten newly discovered wet AMD patients were selected as observation group, 10 patients without fundus disease and 10 patients with systemic disease as control group. The peripheral venous blood was extracted and the (PBMC), of mononuclear cells was extracted. The CD34 cells were further extracted by adenovirus transfection and I PSC was induced and identified. Real time quantitative PCR (qRT-PCR) was used to detect the relative expression of miR-126 in 10 RNSC at different stages of induction and development. Results RNSCs were successfully induced and differentiated from peripheral blood of patients with wet AMD disease, and the difference of relative expression of miR-126 at different induction time in observation group was statistically significant (P0.05), and that in control group was different in different miR-126 induction time (P0.05). There was no significant difference in the relative expression of miR-126 between the two groups (P0.05); there was significant difference in the relative expression of miR-126 between the observation group and the control group at different induction time (P0.05). Conclusion the expression of miR-126 in the early stage of wet AMD patients increased significantly and tended to decrease gradually.
【作者單位】： 深圳市眼科醫(yī)院暨南大學醫(yī)學院深圳眼科中心深圳眼科學重點實驗室;
【基金】：深圳市科技創(chuàng)新委員會國際合作項目(編號:GJHZ20150316150106271) 深圳市“三名工程”項目 廣東省中醫(yī)藥局科研項目(編號:20171239)
【分類號】：R774.5
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